Can high-intensity training protect against exercise-induced oxidation of sarcoplasmic reticulum Ca^<2+>-ATPase?

高强度训练能否防止运动引起的肌浆网C​​a^2-ATP酶氧化？

• 批准号: 16500419
• 负责人: MATSUNAGA Satoshi
• 金额: $ 2.43万
• 依托单位: Osaka City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16500419/
• 关键词: acute exercise; high-intensity training; sarcoplasmic reticulum; protein oxidation; Ca^<2+>-ATPase; タンパク酸化

The cytosolic free Ca^<2+> concentration ([Ca^<2+>]_f) plays a central role in muscle contraction and relaxation. In skeletal muscle, the regulation of [Ca^<2+>]f is accomplished primarily by the kinetics of Ca^<2+> release and uptake by the sarcoplasmic reticulum (SR). Although the fatigue process involves multiple factors and mechanisms, recent evidence implies that functional impairment of the SR is a major contributor to muscle fatigue. We previously observed that a loss of SR function may be attributed, at least partly, to protein oxidation by reactive oxygen species, generation of which is elevated during muscle contraction. However, no published data presently exists that examines whether high-intensity training could protect against deteriorating SR Ca^<2+>2+-ATPase activity and oxidation of Ca^<2+>-ATPase with acute exercise. The purpose of this study was to investigate the influence of high-intensity training and/or a single bout exercise on SR function and oxidation of Ca^<2+>-ATPase.Our in vitro measures on the superficial region of the vastus lateralis revealed that 8 weeks of running training elicited an increase in SR Ca^<2+>-uptake rate without concomitant changes in SR Ca^<2+>-ATPase activity and Ca^<2+>-release rate. These results implicate training-induced decreases in Ca^<2+> efflux from the SR lumen. Despite the extension of the run time to exhaustion, no differences existed in exhaustive exercise-induced reductions in SR Ca^<2+>-uptake ability and increases in carbonyls contained in Ca^<2+>-ATPase between trained and untrained muscles. These findings suggest that high-intensity training might protect Ca^<2+>-ATPase enzyme against oxidative modification that occurs during vigorous muscle contraction.

胞质游离Ca^2+浓度([Ca^2+]_f)在肌肉收缩和松弛中起核心作用。在骨骼肌中，[Ca 2+ ]f 的调节主要通过肌浆网(SR)释放和吸收Ca 2+ 的动力学来完成。尽管疲劳过程涉及多种因素和机制，但最近的证据表明 SR 功能损伤是肌肉疲劳的主要原因。我们之前观察到，SR 功能的丧失可能至少部分归因于活性氧的蛋白质氧化，活性氧的产生在肌肉收缩期间增加。然而，目前尚无公开数据检验高强度训练是否可以防止急性运动时SR Ca 2+ 2+ -ATP酶活性的恶化和Ca 2+ -ATP酶的氧化。本研究的目的是探讨高强度训练和/或单次运动对 SR 功能和 Ca^2+-ATPase 氧化的影响。我们对股外侧肌浅表区域的体外测量揭示了8周的跑步训练引起了SR Ca^<2+>-摄取率的增加，但SR Ca^<2+>-ATP酶活性和Ca^<2+>-释放率没有伴随变化。这些结果表明训练引起的来自SR腔的Ca 2+ 流出减少。尽管延长了跑步至力竭的时间，但在受过训练的肌肉和未经训练的肌肉之间，力竭运动引起的SR Ca^2+-摄取能力的降低和Ca^2+-ATP酶中所含的羰基的增加不存在差异。这些发现表明高强度训练可能保护Ca 2+ -ATP酶免受剧烈肌肉收缩期间发生的氧化修饰。

项目成果

Depressed sarcoplasmic reticulum Ca^<2+>-ATPase activity following high-intensity exercise-The oxidation of protein and the muscle fiber type-

高强度运动后肌浆网Ca^<2>-ATP酶活性降低-蛋白质的氧化和肌纤维类型-

• 发表时间: 2006
• 期刊: Osaka Res.J.Phys.Educ. 44
• 作者: Matsunaga;S.
• 通讯作者: S.

Effects of high-intensity training and acute exercise on in vitro function of rat sarcoplasmic reticulum

• DOI: 10.1007/s00421-006-0381-8
• 发表时间: 2007-01
• 期刊: European Journal of Applied Physiology
• 影响因子: 3
• 作者: S. Matsunaga;Takashi Yamada;T. Mishima;M. Sakamoto;Minako Sugiyama;M. Wada

高強度運動による筋小胞体Ca^<2+>-ATPase活性の減退-タンパク質の酸化と筋線維タイプ-

高强度运动导致肌浆网Ca^2+-ATP酶活性降低 - 蛋白质氧化和肌纤维类型 -

• 发表时间: 2007
• 期刊: 大阪体育学研究 44
• 作者: 中村敏雄;山本徳郎;他;Tokuro Yamamoto et al.;松永 智
• 通讯作者: 松永 智