Research on 3-D analysis of histopathologic specimen for histopathologic diagnosis support

组织病理学标本3D分析支持组织病理学诊断的研究

基本信息

批准号：
16500302
负责人：
TAKAHASHI Masanobu
金额：
$ 2.37万
依托单位：
Shibaura Institute of Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2006
项目状态：
已结题

项目摘要

The purpose of this research were to analyze a histopathologic specimen as a 3-D object, and to develop a histopathologic diagnosis support system which calculate useful information such as 3-D nuclear density independent of the specimen's thickness.Effect of specimen's thickness on the observed (2-D) nuclear density was evaluated theoretically and experimentally, and it was revealed that 1 um of thickness change results in about 10% of change in nuclear density.A method to non-destructively measure the thickness of a histopathologic specimen was realized. The method uses the images captured using an optical microscope so that many pathologists can utilize it.Histopathologic images observed using an optical microscope usually have undesirable blur. A method to reduce blur in the 3-D image was realized. The method uses improved blind deconvolution, and satisfactory results were obtained to construct 3-D models of nuclei.A method to estimate nuclear parameters (3-D nuclear density, average and variance of nuclear radii) was realized. The method utilized only a 2-D microscopic image which pathologists are usually observing so that they can easily use the method. Sequential estimation method in which each parameter is estimated sequentially was also developed to reduce the computation time.Finally, a GUI was developed that enables a user to use the developed method easily. The GUI has functions to semi-automatically extract the nuclear positions and nuclear contours, and to estimate nuclear parameters. Nuclear positions and nuclear contours can easily be corrected using simple mouse operations, so pathologists can easily estimate 3-D nuclear density which is independent of the specimen's thickness.

本研究的目的是将组织病理学标本作为 3-D 对象进行分析，并开发一个组织病理学诊断支持系统，该系统可计算有用的信息，例如与标本厚度无关的 3-D 核密度。标本厚度对观察到的结果的影响(2-D)核密度从理论上和实验上进行了评估，结果表明，1um的厚度变化会导致核密度约10%的变化。一种无损测量核密度的方法实现了组织病理学标本。该方法使用光学显微镜捕获的图像，因此许多病理学家都可以利用它。使用光学显微镜观察的组织病理图像通常会出现不希望的模糊。实现了一种减少 3D 图像模糊的方法。该方法采用改进的盲反卷积，在构建核三维模型方面取得了满意的结果，实现了核参数（三维核密度、核半径平均值和方差）的估计方法。该方法仅利用病理学家通常观察的二维显微图像，以便他们可以轻松地使用该方法。还开发了顺序估计每个参数的方法以减少计算时间。最后，开发了一个GUI，使用户能够轻松地使用所开发的方法。 GUI具有半自动提取核位置和核轮廓以及估计核参数的功能。使用简单的鼠标操作即可轻松校正核位置和核轮廓，因此病理学家可以轻松估计与样本厚度无关的 3-D 核密度。

项目成果

期刊论文数量（41）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Estimation of 3-D nuclear density of hepatic histopathological specimen-Improvement of accuracy-

肝脏组织病理学标本的 3-D 核密度估计-准确性的提高-

DOI：
发表时间：
2005
期刊：
Forum on Information Technology 2005 (FIT2005)
影响因子：
0
作者：
Takashi Yoshimi;Masanobu Takahashi;Masayuki Nakano
通讯作者：
Masayuki Nakano

Estimation of 3-D nuclear density from a 2-D image of hepatic histopathological specimen

从肝脏组织病理学标本的 2-D 图像估计 3-D 核密度

DOI：
发表时间：
2005
期刊：
Proceedings of the 3^<rd> European Medical ＆ Biological Engineering Conference (EMBEC2005)
影响因子：
0
作者：
Takashi Yoshimi;Masanobu Takahashi;Masayuki Nakano
通讯作者：
Masayuki Nakano

標本厚さに影響されない細胞密度・核密度の推定

不受样本厚度影响的细胞密度和核密度的估计

DOI：
发表时间：
2006
期刊：
日本病理学会会誌 95・1
影响因子：
0
作者：
高橋正信;中野雅行
通讯作者：
中野雅行

Quantification of reticulin fibers in hepatic histopathological specimen

肝组织病理学标本中网状纤维的定量

DOI：
发表时间：
2005
期刊：
Proceedings of Tokyo branch conference of IEICE student's association
影响因子：
0
作者：
Yoshitaka Miyao;Masanobu Takahashi
通讯作者：
Masanobu Takahashi

Nondestructive measurement of the thickness of a pathologic specimen using an optical microscope

使用光学显微镜无损测量病理标本的厚度

DOI：
发表时间：
2005
期刊：
Proceedings of the 3^<rd> European Medical ＆ Biological Engineering Conference (EMBEC2005)
影响因子：
0
作者：
Masanobu Takahashi;Yasuhiro Yoneyama;Masayuki Nakano
通讯作者：
Masayuki Nakano