Effects of the combined exercise, diet and antihypertensive drug therapy on diabetic nephropaty in rats

运动、饮食和降压药物联合治疗对大鼠糖尿病肾病的影响

• 批准号: 14580060
• 负责人: SUZUKI Masato
• 金额: $ 2.18万
• 依托单位: Jikei University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14580060/
• 关键词: Diabetic nepliropaty; exercise therapv; diet therapy; combined exercise and medication therapv; combined exercise and diet therapy; urinary albumine excretion; renal alonierular basement membrane (GBM); 腎糸球体基底膜(GBM)厚; 運動と薬物療法併用; 運動と食事療法併用; 腎糸球体基

The present study was conducted to examine the effects of combination therapy, consisting of exercise, diet and ACE-inbibitor(captopuril), on renal function, BE glucose intolerance(GI) and dyslipidemia associated with diabetes mellitus (DM) in OLETF rats. Thirty-five male rats were randomly assigned to exercise, diet, medication, and combined therapy (Med&Ex, Ex &Diet) groups. Each treatment was conducted on rats 21 td 30 weeks in age. An oral GI test was carried out pre-and post-therapy. Body weight (BW), BP and urine albumin excretion rate (UAE) were observed every three weeks during each treatment. After each treatment, the animals were euthanized using venesection under anesthesia. Kidney, heart and several muscle masses were weighed, and structural changes of the kidney, serum lipids and enzymes activities in quadriceps were examined. Results: The Ex and Med &Ex therapy inhibited increases in BW and improved GI. BP in OLETF rats was decreased to the same level as normal control LET() rats by taking an ACE-inhibitor. Increase in UAE was inhibited in the OLETF-Sed and -Med&Ex groups. Ex therapy improved GI and dyslipidemia, and increased muscle mass and enzymes activities in quadriceps. However, it didn't inhibit the development of DN sufficiently, due to the accompanying increase in BP during exercise. Med therapy inhibited increase in UAE, however, BW was increased and GI and dyslipidemia deteriorated to the same levels as those of the OLETFSed grouj. Med&Ex therapy minimized the disadvantage of exercise. Conclusion: This study demonstrated that elevation of BP during exercise in DM rats was a risk factor for the development of DN. The Med&Ex, diet therapy enhanced the advantage of physical exercise and simultaneously prevented the development of DN.

进行了本研究以检查组合疗法的影响，包括运动，饮食和ACE INBIBITOR（CASTOPURIL）对肾功能，是葡萄糖不耐症（GI）和OLETF大鼠中与糖尿病（DM）相关的血脂异常。将三十五只雄性大鼠随机分配为运动，饮食，药物和联合治疗（MED＆EX，EX，EX和饮食）组。每种疗法均在21 TD的大鼠上进行30周的年龄。治疗前后进行口服胃肠道测试。每次处理期间，每三周观察一次体重（BW），BP和尿白蛋白排泄率（UAE）。每次治疗后，在麻醉下使用静脉内对动物进行安乐死。称重肾脏，心脏和几个肌肉肿块，并检查了肾脏，血清脂质和酶活性的结构变化。结果：EX和MED和EX疗法抑制了BW的增加，并改善了GI。通过服用ACE抑制剂，OLETF大鼠中的BP降低到与正常对照组相同的水平。 OLETF -SED和-MED＆EX组抑制了阿联酋的增加。 EX疗法改善了GI和血脂异常，并增加了股四头肌中的肌肉质量和酶活性。但是，由于运动过程中BP的增加，它并没有充分抑制DN的发展。但是，MED疗法抑制了阿联酋的增加，BW增加了，GI和血脂异常降低到与Oletfsed Grouj的水平相同的水平。 Med＆Ex Therapy最大程度地减少了运动的缺点。结论：这项研究表明，在DM大鼠运动过程中，BP的升高是DN发展的危险因素。 Med＆Ex Diet疗法增强了体育锻炼的优势，并同时阻止了DN的发展。

项目成果

Suzuki, M., Hozumi, N., et al.: "Effects of ACE-inhibitor on diabetic nephropathy during exercise therapy in OLETF rats."ACSM Annual Meeting(Jndianapolis). Friday, June. (2004)

Suzuki, M., Hozumi, N., et al.：“ACE 抑制剂对 OLETF 大鼠运动治疗期间糖尿病肾病的影响。”ACSM 年会（Jndianapolis）。

Suzuki, M., Hozumi, N., et al.: "Effects of ACE-inhibitor on diabetic nephropathy during exercise therapy in OLETF rats"2004 ACSM Annual Meeting(Indianapolis). (発表予定). (2004)

Suzuki, M., Hozumi, N., et al.：“ACE 抑制剂对 OLETF 大鼠运动治疗期间糖尿病肾病的影响”2004 年 ACSM 年会（印第安纳波利斯）（待提交）。

鈴木政登, 木村真規, 柴崎敏昭: "糖尿病性腎症に対する運動および薬物療法併用の影響"第49回日本腎臓学会学術総会. (発売の予定). (2003)

Masato Suzuki、Masaki Kimura、Toshiaki Shibasaki：“运动和药物联合治疗对糖尿病肾病的影响”，第 49 届日本肾病学会年会（预定发布）。

鈴木政登, 穂積紀子他: "Effects of angiotensin-converting enzyme (ACE) in hibitor on diabetic nephropathy (DN) during exercise therapy in OLETF rats"第80回日本生理学会大会. (発売予定). (2003)

Masato Suzuki、Noriko Hozumi 等：“OLETF 大鼠运动治疗期间血管紧张素转换酶 (ACE) 抑制剂对糖尿病肾病 (DN) 的影响”第 80 届日本生理学会年会（待发布）。 (2003)

鈴木政登, 穂積典子 他: "糖尿病性腎症に及ぼす運動とACE阻害薬併用の影響-肥満・糖尿病モデルOLETFラットを用いた研究-"第58回日本体力医学会で発表. (2003)

Masato Suzuki、Noriko Hozumi 等人：“联合运动和 ACE 抑制剂对糖尿病肾病的影响 - 使用肥胖/糖尿病模型 OLETF 大鼠的研究 -”在第 58 届日本体能医学学会年会上发表。