Visualization of the Molecular Properties Using Phenolphthalein Derivatives

使用酚酞衍生物可视化分子性质

基本信息

批准号：
14572003
负责人：
TSUBAKI Kazunori
金额：
$ 1.86万
依托单位：
KYOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

项目摘要

During the past two years, we have investigated three types of visual recognition of guest properties using host compounds, hybrid molecules of phenolphthalein and two crownethers. (i)Sequence-Selective Visual Recognition of Nonprotected Dipeptides; A host molecule has an ability for developing brilliant purple color in the presence of dipeptides with a specific amino acid-sequence containing lysine as a C-terminal. This type of color development could be extended to the detection of oligopeptides of a specific sequence at the N-terminal such as scyliorhiin I and APP_<770>(394-410). (ii)Construction of a Variable Temperature Visual Read-Out System for Sodium and Potassium Ions; We have found the first artificial recognition system in which sodium and potassium ions show opposite behaviors. Thus, the absorbance at around 566 nm by the complex of host and NaOH increased as the temperature decreased. In contrast, color development between host and KOR decreased as the temperature decreased. These phenomena were caused by an equilibrium among the host molecule, a colored complex, and a colorless complex. (iii)Visual Enantiomeric Recognition of β-Amino Alcohols; we have developed a phenolphthalein based phenyl groups substituted crown ethers host. The host molecule has an ability to discriminate chirality of β-amino alcohols by naked-eye detectable color change. We really observed strength of color development of this host molecule and (S)-or (R)-guest was reversed at inversion temperature.

在过去的两年中，我们使用宿主化合物，苯酚胺的混合分子和两个冠状球体研究了三种类型的客体特性识别。（i）对非保护二肽的序列选择性视觉识别；宿主分子具有在具有特定氨基酸序列的二肽存在下含有赖氨酸作为C末端的特定氨基酸序列的能力。这种类型的颜色发育可以扩展到在N末端（例如Scienceiorhiin i和App_ <770>）（394-410）等在N末端的特定序列的寡肽的检测。（ii）构建用于钠和钾离子的可变温度视觉读出系统；我们发现了第一个人工识别系统，其中钠和钾离子表现出相反的行为。这，随着温度降低，宿主和NaOH的复合物在566 nm左右的吸收增加。相比之下，随着温度降低，宿主和KOR之间的颜色发育降低。这些现象是由宿主分子，有色复合物和无色复合物中的含量相等引起的。（iii）视觉对映体对β-氨基醇的识别；我们已经开发了一个基于苯酚的苯基苯基取代的冠状醚宿主。宿主分子具有通过可检测到的颜色变化来区分β-氨基醇的手性。我们确实观察到该宿主分子的颜色发展强度，并且（s）或（r）guest在反转温度下被逆转。