Laser Raman Micro-spectroscope Observation for the Analysis of Resin-dentin Interdiffusion Zone

激光拉曼显微光谱观察分析树脂-牙本质相互扩散区

基本信息

批准号：
14571826
负责人：
MIYAZAKI Masashi
金额：
$ 2.18万
依托单位：
Nihon University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571826/
关键词：
Hybrid Layer Dentin Bonding Raman Microscope Resin penetration Bonding Mechanism レジン浸透性

项目摘要

Adhesion of resin composites to tooth substrate is currently believed to rely on the infiltration of bonding resin into the porous zone, establishing micro-mechanical retention to etched surface. If adhesive resin does not penetrate the demineralized tooth substrate, then the porous layer within this layer will be exposed to oral fluids and vulnerable to degradation. The purpose of this study was to investigate the effect of self-etching primer application duration on resin penetration into dentin and dentin bond strength. After making bonded specimens, sectioned through the bonded interface. The sectioned surfaces were then polished with diamond pastes down to 1.0 μm particle size. Raman spectra were successively recorded along a line perpendicular to the tooth/resin interface. The sample stage was moved in steps of 0.2 μm on a computer controlled X-Y precision table. Additional spectra from samples of tooth and cured bonding resins were recorded for reference. The relative amounts of the hydroxyapatite (960cm-1,P-O), bonding agent (640cm-1,aromatic ring), and alkyl group (1450cm-1,C-H) in the enamel/resin bonding area were calculated. From the Raman spectroscopy, the widths of demineralized dentin increased with prolonged treatment time. The patterns of gradual transition of components were different among the groups. Furthermore, the tooth/resin interface represents a gradual transition of bonding agent from resin side to tooth side. From the results of this study, nonuniform resin infiltration into the etched enamel was detected and the degree of resin infiltration was different among the bonding systems used.

目前认为树脂复合材料对牙齿基质的粘附依赖于粘合树脂渗透到多孔区域中，从而建立蚀刻表面的微机械作用。如果粘合剂保留树脂不渗透脱矿的牙齿基质，则该层内的多孔层。本研究的目的是研究自酸蚀底漆的应用时间对树脂渗透到牙本质和牙本质粘合强度的影响。然后沿着垂直于牙齿/树脂界面的线将切片表面抛光至 1.0 μm 颗粒。样品台以 0.2 μm 的步长移动。记录牙齿和固化粘合树脂样品的附加光谱以供参考。从拉曼光谱计算出牙釉质/树脂结合区域的(960cm-1,P-O)、结合剂(640cm-1,芳香环)和烷基(1450cm-1,C-H),脱矿牙本质的宽度增加。随着治疗时间的延长，各组的成分逐渐转变的模式也不同。此外，牙齿/树脂界面代表了粘合剂的逐渐转变。从本研究的结果来看，检测到蚀刻牙釉质的树脂渗透不均匀，并且所使用的粘合系统的树脂渗透程度不同。