Analysis of expression pattern of fibrocartilage with a view to promoting regenerative medicine of temporomandibular joint
纤维软骨表达模式分析以促进颞下颌关节再生医学
基本信息
- 批准号:15592098
- 负责人:
- 金额:$ 1.54万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:2003
- 资助国家:日本
- 起止时间:2003 至 2005
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Regenerative medicine of the temporomandibular joint (TMJ) is one of the most important subjects in the field of oral and maxillofacial surgery. In order to provide a basis of therapeutic application of TMJ cartilage regeneration, we have investigated in the following projects.(1)Basic study to prepare a chondrocyte proliferation medium : In this project, we used cartilage cells derived from the cartilage of the nasal septum and the remnant auricular cartilage. We have aimed to prepare a chondrocyte proliferation medium that i)does not contain fetal bovine serum (FBS), ii)provides more than a 1000-fold increase in cell numbers, and iii)makes use of a combination of commercially available growth factors that has been proven to be effective for clinical use. As a result, a combination of FGF-2, insulin, and IGF- 1 synergistically enhanced the proliferation. Furthermore, we showed that a combination of BMP-2, insulin, and PTH possessed promotional effects on proliferation of hypertrophic … More differentiation of chondrocyte. Finally, we evaluated the property of the scaffold using some kinds of hydro-gel on cartilage regeneration.(2)Understanding the molecular mechanism of chondrocyte differentiation : We have investigated a novel role of CGK II in hypertrophic differentiation of chondrocytes and a critical function of Cdk 6 as a negative regulator of differentiation of osteoblast, osteoclast and chonrocyte. As a result, CGK II, a molecular switch, coupled the cessation of proliferation and the start of hypertrophic differentiation of chondrocytes through attenuation of Sox 9 function. Cdk 6 was a critical regulator of BMP-2-induced osteoblast differentiation by Smads-mediated down-regulation and, RAW cells overexpressing Cdk 6 resisted RANKL-induced osteoclastgenesis ; however, cell cycle regulation was not affected by the levels of Cdk 6 overexpression. In conclusion, we have demonstrated in vitro evidence of the importance of these molecules in proliferation and differentiation of bone tissue. Less
颞下颌关节(TMJ)再生医学是口腔颌面外科领域最重要的学科之一,为了给颞下颌关节软骨再生的治疗应用提供基础,我们进行了以下项目的研究。(1)制备软骨细胞增殖培养基的基础研究:在该项目中,我们使用源自鼻中隔软骨和残余耳软骨的软骨细胞。制备软骨细胞增殖培养基,i) 不含胎牛血清 (FBS),ii) 使细胞数量增加 1000 倍以上,以及 iii) 使用已被证明可有效促进细胞生长的市售生长因子组合结果,FGF-2、胰岛素和 IGF-1 的组合可协同增强增殖。 PTH 对软骨细胞肥大分化具有促进作用。最后,我们评估了使用某些水凝胶的支架对软骨再生的性能。(2)了解软骨细胞分化的分子机制:我们研究了一种新的软骨细胞分化的分子机制。 CGK II 在软骨细胞肥大分化中的作用以及 Cdk 6 作为成骨细胞、破骨细胞和软骨细胞分化负调节因子的关键功能。 CGK II 是一种分子开关,通过减弱 Sox 9 功能,将增殖停止和软骨细胞肥大分化开始联系起来,Cdk 6 是 BMP-2 介导的下调所诱导的成骨细胞分化的关键调节因子。过表达 Cdk 6 的 RAW 细胞可抵抗 RANKL 诱导的破骨细胞生成;然而,细胞周期调节不受 Cdk 6 过表达水平的影响。结论是,我们已经在体外证明了这些分子在骨组织增殖和分化中的重要性。
项目成果
期刊论文数量(33)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Involvement of cyclic guanosine monophosphate-dependent protein kinase II in chondrocyte hypertrophy during endochondral ossification
- DOI:10.3109/s10165-005-0436-4
- 发表时间:2005-12
- 期刊:
- 影响因子:2.2
- 作者:F. Kugimiya;H. Chikuda;Satoru Kamekura;T. Ikeda;K. Hoshi;T. Ogasawara;Kozo Nakamura;U. Chung;H. Kawaguchi
- 通讯作者:F. Kugimiya;H. Chikuda;Satoru Kamekura;T. Ikeda;K. Hoshi;T. Ogasawara;Kozo Nakamura;U. Chung;H. Kawaguchi
Mutation in cGMP-dependent protein kinase II causes dwarfism in a rat mutant KML through uncoupling of proliferation and differentiation of chondrocytes.
cGMP 依赖性蛋白激酶 II 的突变通过软骨细胞增殖和分化的解偶联导致大鼠突变 KML 侏儒症。
- DOI:
- 发表时间:2005
- 期刊:
- 影响因子:0
- 作者:Hikiji H;Hirotaka chikuda et al.
- 通讯作者:Hirotaka chikuda et al.
Bone morphogenetic protein 2-induced osteoblast differentiation requires Smad-mediated down-regrulation of Cdk6.
骨形态发生蛋白 2 诱导的成骨细胞分化需要 Smad 介导的 Cdk6 下调。
- DOI:
- 发表时间:2004
- 期刊:
- 影响因子:0
- 作者:Toru Ogasawara et al.
- 通讯作者:Toru Ogasawara et al.
Yu Koshizuka, Takashi Yamada, Kazuto Hoshi, Toru Ogasawara, et al.: "Cystatin 10,a novel chondrocyte-specific protein, may promote the last steps of the chondrocyte differentiation pathway"J.Biol.Chem. 278. 48259-48266 (2003)
Yu Koshizuka、Takashi Yamada、Kazuto Hoshi、Toru Ogasawara 等人:“Cystatin 10,一种新型软骨细胞特异性蛋白,可能促进软骨细胞分化途径的最后步骤”J.Biol.Chem。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Synergistic effects of FGF-2 with insulin of IGF-I on the proliferation of human auricular chondrocytes.
FGF-2与IGF-I胰岛素对人耳软骨细胞增殖的协同作用。
- DOI:
- 发表时间:2005
- 期刊:
- 影响因子:0
- 作者:Takahashi;T.;et al.
- 通讯作者:et al.
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YODA Tetsuya其他文献
顎関節疾患におけるMRIの活用と留意点
MRI在颞下颌关节疾病中的应用及注意事项
- DOI:
- 发表时间:
2022 - 期刊:
- 影响因子:0
- 作者:
TOMOMATSU Nobuyoshi;WACHI Kotaro;WAKE So;TAKAHARA Namiaki;YOSHITAKE Hiroyuki;YODA Tetsuya;儀武啓幸 - 通讯作者:
儀武啓幸
A case of infectious arthritis of the temporomandibular joint that spread from malignant external otitis
恶性外耳炎所致颞下颌关节感染性关节炎一例
- DOI:
10.5794/jjoms.68.15 - 发表时间:
2022 - 期刊:
- 影响因子:0
- 作者:
TOMOMATSU Nobuyoshi;WACHI Kotaro;WAKE So;TAKAHARA Namiaki;YOSHITAKE Hiroyuki;YODA Tetsuya - 通讯作者:
YODA Tetsuya
YODA Tetsuya的其他文献
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{{ truncateString('YODA Tetsuya', 18)}}的其他基金
Proteome analysis and energy dispersive X-ray analysis in masticatory muscle tendon-aponeurosis hyperplasia
咀嚼肌腱腱膜增生的蛋白质组分析和能量色散X射线分析
- 批准号:
24593005 - 财政年份:2012
- 资助金额:
$ 1.54万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
The analysis of pathological condition in masticatory muscle tendon-aponeurosis hyperplasia using HUMARA assay and immunostaining methods.
采用 HUMARA 检测和免疫染色方法分析咀嚼肌腱腱膜增生的病理状况。
- 批准号:
20592344 - 财政年份:2008
- 资助金额:
$ 1.54万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
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10637251 - 财政年份:2023
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Tissue-engineered Aged B Cell Immune Organoid to Study Antibody Secreting Cell Differentiation Trajectory
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