Neurogenic control of cerebrovascular bed : Study of Klotho type mouse

脑血管床的神经源性控制：Klotho型小鼠的研究

基本信息

批准号：
15591550
负责人：
MAEDA Minoru
金额：
$ 1.86万
依托单位：
Juntendo University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591550/
关键词：
Klotho mouse Wild mouse 4 weeks and 6 weeks old [^<14>C]DG LCGU Nuerogeneric control of cerebrovascular bed basal forebrain 脳の組織学的変化

项目摘要

A defect in klotho gene expression in the mouse results in a syndrome that resembles human ageing, including a short life span, inferitility, arteriosclerosis, skin atrophy, osteoporosis and emphysema.Neurohistological, physiological and cerebral metabolic parameters were investigated in the klotho mouse in comparison with the wild mouse.i) Histological and physiological studies ; In the klotho mice, neurons, axons and S-100 stain were sparse in the neocortex and the hypocampus. The klotho mice (both in 4 weeks and 6 weeks old) were significantly light in weight and low in blood sugar.ii) Local cerebral glucose utilization (LCGU) ; On the base of preliminary experiments, we applied the modified deoxyglucose method to the wild and transgenic mice. 30μCi of 2DG was injected intraperitoneally and 5μL mixed blood samples from the cut end of tail were corrected at 0, 10, 20, 30 and 45 minutes. The lumped constants for hypoglycemic mice were previously determined in the hypoglycemic rat (Suda et al, 1990).(1) LCGU of 4 weeks old (w.o.) mice ; klotho type (kl/kl) vs wild type (+/+), LCGU in the thalamus, caudate putamen, inferior colliculus, cerebellar cortex and internal capsel of the klotho mice were not significantly changed. LCGU in the other structures including neocortex were significantly decreased in comparison with the wild mice.(2) LCGU of 6 weeks old mice ; klotho (kl/kl) vs wild (+/+), LCGU in whole brain of the klotho mice were significantly, remarkably lower than those of the wild mice.(3) The comparison of glucose utilization of 4 w.o. and 6 w.o. mice in klotho type revealed significant decrease in 6 w.o. mice.iii) In our previous studies, it is proposed that the basal forebrain plays an important role for control of cerebrovascular bed. Glucose utilization of these specific areas in younger klotho mice remaines to be further investegation.

小鼠中 klotho 基因表达的缺陷会导致类似于人类衰老的综合症，包括寿命短、不育、动脉硬化、皮肤萎缩、骨质疏松和肺气肿，并在 klotho 小鼠中研究了神经组织学、生理学和脑代谢参数。 i) 组织学和生理学研究；在 klotho 小鼠中，新皮质中的神经元、轴突和 S-100 染色稀疏klotho小鼠（4周龄和6周龄）体重明显减轻且血糖较低。ii）局部脑葡萄糖利用（LCGU）；在初步实验的基础上，我们应用改良的脱氧葡萄糖方法对野生小鼠和转基因小鼠腹腔注射30μCi的2DG，并在0、10、20时从尾部切端取5μL混合血进行校正。先前在低血糖大鼠中测定了 30 和 45 分钟的集总常数（Suda 等人，1990）。（1）4 周龄（w.o.）小鼠的 LCGU；klotho 型（kl/kl）与野生型（ +/+), LCGU 位于丘脑、尾壳核、下丘、小脑皮质和内囊klotho小鼠的其他结构包括新皮质的LCGU均较野生小鼠显着降低。(2)6周龄小鼠的LCGU；klotho(kl/kl)与野生小鼠(+/+)相比，LCGU (3)klotho型小鼠全脑葡萄糖利用率显着低于野生型小鼠。(3)比较4只w.o.和6.o.o小鼠的葡萄糖利用率明显下降。 6 只小鼠。iii) 在我们之前的研究中，提出基底前脑在控制脑血管床中发挥重要作用，这些特定区域的葡萄糖利用仍有待进一步研究。