Study on carbon monoxide toxicity independent of hypoxia

不依赖缺氧的一氧化碳毒性研究

• 批准号: 15590590
• 负责人: HARA Shuichi
• 金额: $ 2.24万
• 依托单位: Tokyo Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15590590/
• 关键词: Carbon monoxide; Hydroxyl radical; Hypoxia; Glutamate; Calcium ion; Rat striatum; pO_2; Blood flow; ヒドロキシルラジカル; ラット; 線条体; 酸素分圧; ドパミン; テトロドトキシン

1. Carbon monoxide (CO) poisoning caused by CO exposure at 3000 ppm for 40 min stimulated hydroxyl radical (・OH) generation in rat striatum.(1) The CO-induced ・OH generation was suppressed by a voltage-dependent Na^+ channel blocker, tetrodotoxin (TTX).(2) CO poisoning increased the extracellular glutamate (Glu) level, but Glu receptor antagonists (MK-801 and NBQX) had no effect on the ・OH generation.(3) α-Methyl-p-tyrosine, clorgyline (Inhibitors of DA synthesis and oxidative metabolism, respectively) and dexamethazone 21-phosphate (a phospholipase A2 inhibitor) had no effect on the CO-induced ・OH generation.(4) Nitric oxide (NO) synthase inhibitors and an NO precursor, L-arginine (L-Arg), and its enantiomer, D-Arg, had complex effects on the CO-induced ・OH generation. (This might be at least partly due to the nature of the striatum that may have a low ability to enhance NO production by utilising exogenous L-Arg.)(5) The CO-induced ・OH generation was more slowly induced by the CO poisoning in the absence of extracellular Cat than in the presence of extracellular Ca^<2+>. The ・OH generation in the absence of extracellular Ca^<2+> was suppressed by dantrolene (an inhibitor of Ca^<2+> release from sarcoplasmic reticulum).(6) Hypoxic hypoxia (HH; 5% O_2 for 40 min) stimulated ・OH generation in the striatum, though more wearly than the CO poisoning.2. CO (3000 ppm for 40 min) and HH (5% O_2 for 40 min) induced similar changes in O_2 contents and oxyhemoglobin levels, but not pO_2, in arterial blood and extracellular amino acids (Glu, taurine and alanine), the blood flow and pO_2 in the striatum.

1。在3000 ppm的碳中毒（CO）中毒40分钟刺激了大鼠纹状体中的羟基自由基（・ OH） ）对OH的生成没有影响。哦，没有前体，L-精氨酸（L-arg）及其对映体D-arg对纹状体的自然作用具有复杂的作用。在没有细胞外Ca^<2+>的情况下，外源性l-arg。 ; 5％O_2持续40分钟 / OH在疲倦中的生成比CON.2 ，在人工血液和细胞外氨基酸（GLU，牛磺酸和丙氨酸）中，纹状体中的血流和PO_2。

项目成果

Characterization of hydroxyl radical generation in the striatum of free-moving rats due to carbon monoxide poisoning, as determined by in vivo microdialysis

• DOI: 10.1016/j.brainres.2004.05.047
• 发表时间: 2004-08-06
• 期刊: BRAIN RESEARCH
• 影响因子: 2.9
• 作者: Hara, S;Mukai, T;Endo, T
• 通讯作者: Endo, T