The sensitivity to endocrine disruptors and the establishment of sensitive bioassay for endocrine disruptors in poultry

家禽内分泌干扰物敏感性及内分泌干扰物敏感生物测定方法的建立

• 批准号: 15580232
• 负责人: SUGIYAMA Toshie
• 金额: $ 2.3万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15580232/
• 关键词: chicken; Japanese quail; endocrine disruptor; sensitivity; bioassay; medullary bone; receptor; ビテロゲニン; バイオアッセイ系; ダイオキシン受容体; DDE; ダイオキシン; 卵管; chicken; Japanese quail; endocrine disruptor; sensitivity; bioassay; medullary bone; receptor; ビテロゲニン; バイオアッセイ系; ダイオキシン受容体; DDE; ダイオキシン; 卵管

1.Aromatic hydrocarbon receptor (AhR ; dioxin receptor) mRNA expression with aging.We detected the expression of AhR mRNA in various tissues of female chickens with aging (0 to 390 days old) by RT-PCR. As a result, AhR mRNA was detected in gizzard, liver, lung, kidney and heart during all stages. However, the oviduct and bone marrow of female chickens represented no AhR mRNA at 0-day stage and less at 30-90-day stages, respectively. These results suggest that the sensitivities of oviduct and bone marrow to dioxin are altered according to the age of female chickens.2.Expression and localization of AhR in oviduct of laying chickens.AhR expression was detected in magnum, isthmus and shell gland of oviducts by western blotting analysis. Especially in isthmus and shell gland, AhR was strongly localized in the cytoplasm of their epithelial cells. These results suggest that dioxin directly disrupts the metabolism of isthmus and shell gland, resulting in the eggshell weakness.3.In Vivo bioassa … More y of endocrine disruptors using avian medullary bone.The treatment of male Japanese quail with DDE (10mg/day) for 7 days induced medullary bone formation in marrow cavities of femurs. Concomitantly, the treatment increased the serum vitellogenin levels. These results suggest that avian medullary bone formation is superior as in vivo bioassay model of endocrine disruptors.4.Expression and localization of endocrine disrupter receptors in avian medullary bone.Estrogen receptor (α and β) and androgen receptor are well known as receptors for endocrine disruptor. In the present study by in situ hybridization and immunohistochemical analyses, both receptors (especially α subtype in estrogen receptor) were localized in osteoblasts of chicken medullary bone. These results suggest that endocrine disruptors directly act on osteoblastic bone formation.5.RT-PCR analysis of vitellogenin mRNA expression in avian liver.We tried to detect vitellogenin mRNA expression in liver of male quails treated with estrogen receptor agonists. As a result, vitellogenin mRNA expression was induced by estrogen receptor α agonist but not estrogen receptor β agonist. This indicates that vitellogenin synthesis in liver is stimulated through estrogen receptor α. Less

1.氨基碳氢化合物受体（AHR；二恶英受体）mRNA表达，衰老。我们通过RT-PCR检测到AHR mRNA的表达（0至390天大）。结果，在各个阶段，在Gizzard，Gizzard，Liver，Lung，Hearney和Heart中都检测到AHR mRNA。然而，雌性鸡的卵形和骨髓在0天阶段没有AHR mRNA，分别在30-90天阶段较少。 These results suggest that the sensitivity of oviduct and bone marrow to dioxin are altered according to the age of female chickens.2.Expression and localization of AhR in oviduct of laying chickens.AhR expression was detected in magnum, isthmus and shell gland of oviducts by western blotting analysis.Especially in isthmus and shell gland, AhR was strongly localized in the cytoplasm of their epithelial细胞。 These results suggest that dioxin directly disrupts the metabolism of isthmus and shell gland, resulting in the eggshell weakness.3.In Vivo bioassa … More y of endocrine disruptors using avian medullary bone.The treatment of male Japanese quail with DDE (10mg/day) for 7 days induced medullary bone formation in marrow cavities of femurs.同时，治疗增加了血清卵黄蛋白水平。这些结果表明，禽髓骨形成是内分泌干扰物的体内生物测定模型的优越性。4。在鸟类髓质骨中内分泌干扰物的表达和定位。雌激素受体（α和β）和雄激素受体是内分泌破坏者的受体众所周知的。在原位杂交和免疫组织化学分析的本研究中，两个受体（尤其是雌激素受体中的α亚型）均位于鸡肉髓质骨的成骨细胞中。这些结果表明，内分泌干扰物直接作用于成骨细胞骨形成。5.RT-PCR分析禽肝脏中维替芬蛋白mRNA的表达分析。我们试图检测用雌激素受体激动剂治疗的雄性鹌鹑的肝脏中的维替芬蛋白mRNA表达。结果，雌激素受体α激动剂诱导了杀菌蛋白mRNA的表达，而不是雌激素受体β激动剂。这表明通过雌激素受体α刺激肝脏中的硫化蛋白合成。较少的