Signaling mechanism of fruit body formation of basidiomycetous mushrooms

担子菌子实体形成的信号机制

• 批准号: 15580073
• 负责人: SEN Kikuo
• 金额: $ 2.11万
• 依托单位: Shinshu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15580073/
• 关键词: basidiomycetes; mushrooms; Schizophyllum commune; fruit body formation; signal transduction; tryptophan; indole; caffeine; イドール; basidiomycetes; mushrooms; Schizophyllum commune; fruit body formation; signal transduction; tryptophan; indole; caffeine; イドール

This study aims to elucidate the signaling mechanism of sexual development of basidiomycetous mushrooms from mating to fruit body formation. Here, stimulation factors for sexual development of the model basidiomycete, Schizophyllum commune, were researched from both viewpoints of environmental factors (external signal factors) and genetic factors.1. Environmental factors (external signal factors)Effects of external signal factors on fruit body formation of S.commune were examined by addition of indole-related compounds to agar media. At the low concentration of 100μg/ml, effects on fruit body formation of indole (Ind), 3-indoleacetic acid (IAA), 3-indolecarboxylic acid (ICA), 3-indolepropionic acid (IPA) and tryptophan (Trp) were as follows : IBA>ICA≧IPA≧IAA>Ind≧Trp. This result showed that compounds containing a structural moiety of indole stimulated fruit body formation.2. Genetic factorsTrp-systhesizing gene of S.commune, TRP1, suppresses abnormality in sexual development (abnormal mating and nuclear migration) induced by indole-resistant mutation (ind1) and caffeine-resistant mutation (cfn1). Sequencing analysis of the TRP1 showed that its product was an enzyme with three catalytic regions. One of the three regions, IGPS (indole-3-glycerol phosphate synthetase) had ability to suppress the abnormal sexual development. Therefore, highly expressed IGPS gene was constructed, and dikaryon with high expression of IGPS was made using the IGPS gene constructed. The dikaryon with high expression of IGPS formed fruit bodies 10 days earlier than dikaryons with no or usual expression of IGPS externally introduced. This result showed that the IGPS region in TRP1 had ability to stimulate sexual development of wild-typed strains as well as the ability to suppress abnormal sexual development of ind1 and cfn1 mutant strains.

这项研究旨在阐明碱性肌肉室性发育的信号传导机制，从交配到水果体形成。在这里，从环境因素（外部信号因素）和遗传因素的角度研究了模型碱性菌的性发展的刺激因素。1。通过向琼脂培养基添加与吲哚相关的化合物，检查了外部信号因子对果实体形成的环境因素（外部信号因素）。在低浓度的100μg/mL下，对吲哚的形成（IND），3- indoleaceticac（IAA），3-吲哚羧酸（ICA），3- indolopropionic酸（IPA）和色氨酸（TRP）的影响如下如下：IBA> ICA> ICA> ICA> ICA> ICA> ICA> IPA ipa ipa ipa rpp。该结果表明，含有吲哚刺激的果实形成的结构部分的化合物2。遗传因素TRP1的遗传因素基因TRP1的基因抑制了耐吲哚突变（IND1）和抗咖啡因的突变（CFN1）引起的性发育异常（异常交配和核迁移）。 TRP1的测序分析表明其产物是具有三个催化区域的酶。三个区域之一，IGP（吲哚-3-甘油磷酸合成酶）具有抑制异常性发展的能力。因此，构建了高表达的IGP基因，并使用构建的IGP基因制成具有高表达IgP的二骨。比二核酸二核酸甲藻（Igps）高表达的二卡里亚（Dikaryon）比二卡里亚（Dikaryons）早于没有或通常在外部引入的IGP表达。该结果表明，TRP1中的IGP区域具有刺激野生菌株的性发育以及抑制IND1和CFN1突变菌株异常性发育的能力。