Signaling mechanism of fruit body formation of basidiomycetous mushrooms

担子菌子实体形成的信号机制

基本信息

批准号：
15580073
负责人：
SEN Kikuo
金额：
$ 2.11万
依托单位：
Shinshu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15580073/
关键词：
basidiomycetes mushrooms Schizophyllum commune fruit body formation signal transduction tryptophan indole caffeine イドール

项目摘要

This study aims to elucidate the signaling mechanism of sexual development of basidiomycetous mushrooms from mating to fruit body formation. Here, stimulation factors for sexual development of the model basidiomycete, Schizophyllum commune, were researched from both viewpoints of environmental factors (external signal factors) and genetic factors.1. Environmental factors (external signal factors)Effects of external signal factors on fruit body formation of S.commune were examined by addition of indole-related compounds to agar media. At the low concentration of 100μg/ml, effects on fruit body formation of indole (Ind), 3-indoleacetic acid (IAA), 3-indolecarboxylic acid (ICA), 3-indolepropionic acid (IPA) and tryptophan (Trp) were as follows : IBA>ICA≧IPA≧IAA>Ind≧Trp. This result showed that compounds containing a structural moiety of indole stimulated fruit body formation.2. Genetic factorsTrp-systhesizing gene of S.commune, TRP1, suppresses abnormality in sexual development (abnormal mating and nuclear migration) induced by indole-resistant mutation (ind1) and caffeine-resistant mutation (cfn1). Sequencing analysis of the TRP1 showed that its product was an enzyme with three catalytic regions. One of the three regions, IGPS (indole-3-glycerol phosphate synthetase) had ability to suppress the abnormal sexual development. Therefore, highly expressed IGPS gene was constructed, and dikaryon with high expression of IGPS was made using the IGPS gene constructed. The dikaryon with high expression of IGPS formed fruit bodies 10 days earlier than dikaryons with no or usual expression of IGPS externally introduced. This result showed that the IGPS region in TRP1 had ability to stimulate sexual development of wild-typed strains as well as the ability to suppress abnormal sexual development of ind1 and cfn1 mutant strains.

本研究旨在阐明担子菌从交配到子实体形成的性发育的信号机制，从环境因素（外部信号因素）和模型担子菌（Schizophyllum commune）的性发育刺激因素进行研究。 1.环境因素（外部信号因素）通过添加吲哚相关化合物来考察外部信号因素对S.commune子实体形成的影响。琼脂培养基中100μg/ml的低浓度对吲哚(Ind)、3-吲哚乙酸(IAA)、3-吲哚甲酸(ICA)、3-吲哚丙酸(IPA)和色氨酸(Trp)的子实体形成有影响。 )如下： IBA>ICA≥IPA≥IAA>Ind≥Trp 该结果表明，化合物含有a。吲哚刺激子实体形成的结构部分。2。遗传因素S.commune的Trp合成基因TRP1抑制由吲哚抗性突变（ind1）和咖啡因抗性突变（ind1）引起的性发育异常（异常交配和核迁移）。 TRP1的测序分析显示其产物是具有三个催化区域之一的酶，IGPS。 (吲哚-3-甘油磷酸合成酶)具有抑制异常性发育的能力，因此构建了高表达的IGPS基因，并且使用构建的IGPS基因早10天制备了具有高表达的IGPS的双核体。没有或通常从外部引入IGPS的表达。该结果表明TRP1中的IGPS区域具有刺激性发育的能力。野生型菌株以及抑制 ind1 和 cfn1 突变菌株异常性发育的能力。