Antioxidant properties and factors of allspice constituents

五香粉成分的抗氧化特性及影响因素

• 批准号: 15500547
• 负责人: KIKUZAKI Hiroe
• 金额: $ 1.86万
• 依托单位: Osaka City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15500547/
• 关键词: allspice; Pimenta dioica; flavonoid; gallic acid related compounds; phenylpropanoid; inhibition of lipid autoxidation; radical scavenging activity; antioxidant properties; 抗酸化性; 自動酸化; ドレッシング; DPPHラジカル補足活性; O_2^-ラジカル補足活性; リポソーム膜酸化抑制活性

This study was aimed to determine the structures of allspice constituents and elucidate their antioxidant properties as well as the factors of antioxidant activity. Dried allspice was successively extracted with n-hexane, dichloromethane and 70% aqueous acetone at room temperature. After removing acetone from the 70% aqueous acetone extract, the resulting aqueous residue was separated into the dichloromethane-soluble, ethyl acetate-soluble and water-soluble fractions. The ethyl acetate-soluble fraction which showed a strong DPPH radical scavenging activity and a high inhibitory effect on the autoxidation of methyl linoleate in bulk system was purified using repeated chromatographic techniques to give twenty compounds including two phenylpropanoids, eleven flavonoids and seven gallic acid related compounds. Their structures were elucidated on the basis of some spectral data. Among them, five are new and fifteen are known. However ten known compounds were first isolated from allspice. Th … More e antioxidant properties of these isolated compounds together with seventeen compounds which have been already isolated in our laboratory were evaluated using several different methods. The DPPH radical scavenging activity was dependent on the number of ortho-diphenols. The O_2^- radical scavenging activity of compounds with 1,2,3-trihydroxyphenyl groups is stronger than that of compounds with 1,2-dihydroxyphenyl groups. Ortho-diphenol structure was contributed to the inhibitory effects on autoxidation of methyl linoleate in bulk system. When measured at 90℃, the high polar compounds such as tannin and flavonoid glycosides showed slight activity, which suggested that affinity to substrate oil might have some influence on their activity in bulk system at 90℃. Such phenomenon was not observed when measured at 40℃. Tannin showed very strong activity toward liposome oxidation induced by AAPH. In the case of flavonoid, quercetin related compounds showed stronger activity than myricetin related compounds. In addition, the inhibitory effects of six allspice constituents on the autoxidation of dressing at 20℃ were measured. The activity order was consistent with the scavenging activity order against DPPH and in this case the polarity of tested compounds did not influence the activity. In conclusion, compounds with ortho-diphenol structure contributed to the antioxidant effect of allspice, and their antioxidant properties might be governed by their radical scavenging abilities and polarities. Less

这项研究的目的是确定五多球成分的结构，并阐明其抗氧化特性以及抗氧化活性的因素。在室温下，干燥的绿色面粉成功提取了N-己烷，二氯甲烷和70％的丙酮。从70％的丙酮提取物中取出丙酮后，将所得的水居住在二氯甲烷 - 溶解，乙酸乙酯 - 溶解乙酸乙酯和水溶性馏分中。使用重复的色谱技术纯化了乙酸乙酸乙酯溶性分数，显示出强大的DPPH自由基清除活性，并对散装系统中甲基linoleate的自动氧化产生高抑制作用，以提供两种化合物，包括两种化合物，包括两种苯基型，包括两种苯基型，包括十一类黄酮酸和七种富甲酸相关的化合物。其中，有五个是新的，而十五个是众所周知的。但是，首先将十种已知的化合物与五香粉分离。使用几种不同的方法评估了这些分离化合物的更多E抗氧化特性以及已经在我们的实验室中已经分离出来的17种化合物。 DPPH自由基清除活性取决于邻苯酚的数量。 O_2^ - 具有1,2,3-三羟基苯基的化合物的自由基清除活性强于1,2-二羟基苯基的化合物。邻苯酚结构有助于抑制对大量系统中甲基甲基植物的自氧化作用。当在90℃测量时，高极性化合物（如单宁和类黄酮糖苷）显示出轻微的活性，这表明对底物油的亲和力可能会影响其在90°处的散装系统中的活性。单宁表现出对AAPH诱导的脂质体氧化的非常强大的活性。在类黄酮的情况下，槲皮素相关的化合物比与米他汀相关的化合物表现出更强的活性。另外，测量了六个千层一致性对20°处敷料自动氧化的抑制作用。活动顺序与针对DPPH的清除活动顺序一致，在这种情况下，测试化合物的极性不会影响活性。结论，具有正二酚结构的化合物有助于绿色的抗氧化作用，其抗氧化特性可能受其根本的清除能力和极性的控制。较少的

项目成果

Antioxidative polyphenols from berries of Pimenta dioica

来自 Pimenta dioica 浆果的抗氧化多酚

• 发表时间: 2004
• 期刊: BioFactors 22
• 作者: Y.Miyajima;H.Kikuzaki et al.
• 通讯作者: H.Kikuzaki et al.