Pathophysiology of the treatment of transient cerebral ischemia

短暂性脑缺血治疗的病理生理学

基本信息

批准号：
15500230
负责人：
KUROIWA Toshihiko
金额：
$ 2.11万
依托单位：
Tokyo Medical and Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

项目摘要

We have evaluated postischemic neurological and cognitive dysfunction after mild focal cerebral ischemia in Mongolian gerbil. Mongolian gerbils underwent the left common carotid artery occlusion (CCAO) for 10, 15, or 20 min. A battery of neurological tests including T maze test, bilateral asymmetry test, open field test, elevated body swing test and methamphetamine (MP)-induced rotation test was performed to evaluate the neurological and cognitive dysfunction including f extrapyramidal motor dysfunction.1.Postischemic motor and somatosensory functions were significantly correlated with regional infarction volumes in the corresponding cortical regions. In gerbils, visual abnormality could be independently detected by the T-maze test. Such regional analyses of ischemic lesions would be useful for investigating the functional outcomes of stroke therapy.2.Biased rotation behavior is a sensitive parameter of the extent of striatal injury after focal cerebral ischemia provided the lesion is … More not extended to the ipsilateral cortex. UP-induced rotation in rodents probably coordinates with the extrapyramidal motor dysfunction after striatal infarction in patients with vascular Parkinsonism.3.The total infarction volumein the neuronal stem cell -grafted animals was significantly lower than that in controls. Approximately 8% of the grafted stem cell urvived, mainly in areas of selective neuronal death, and were costained with antibodies against neuronal nuclei antibody, microtubule associated protein, glial fibrillary acidic protein. Synaptic structures between stem cell derived neurons and host neurons were bserved. Furthermore, gradual improvement of neurologic functions was observed clearly in the stem cell grafted animals, compared to that in controls. Human neuronal stem cell remarkably improved neurologic functions after focal ischemia in the Mongolian gerbil, and maintained their abilities to migrate around the infarction, differentiate into mature neurons, and form synapses with host neuronal circuits. Less

我们对蒙古沙鼠进行左颈总动脉闭塞（CCAO）10、15或20分钟后进行了一系列神经学测试，包括T迷宫测试、双侧不对称性，评估了其缺血后神经和认知功能障碍。进行试验、旷场试验、高架摆动试验和甲基苯丙胺（MP）诱导的旋转试验来评估神经和认知功能障碍，包括锥体外系运动功能障碍。1.沙鼠的缺血后运动和体感功能与相应皮质区域的局部梗塞体积显着相关，通过T迷宫测试可以独立检测视觉异常，这种对缺血性病变的区域分析将有助于研究。 2. 偏向旋转行为是局灶性脑缺血后纹状体损伤程度的敏感参数，前提是损伤未延伸至 UP 诱导的同侧皮层。啮齿动物的旋转可能与血管性帕金森病患者纹状体梗塞后的锥体外系运动功能障碍相协调。3.神经元干细胞移植动物的总梗塞体积显着低于对照组，约8％的移植干细胞存活。，主要在选择性神经元死亡区域，并与抗神经元细胞核抗体、微管相关蛋白、神经胶质纤维突触酸性蛋白的抗体共染色。此外，与对照组相比，干细胞移植动物的神经功能明显改善，而蒙古沙鼠局灶性缺血后的神经功能明显改善。，并保持它们在梗塞周围迁移、分化为成熟神经元以及与宿主神经元回路形成突触的能力。

项目成果

期刊论文数量（46）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Human neuronal stem/progenitor cells, expanded in long-term neurosphere culture, promote functional recovery after focal ischemia in Mongolian gerbils.

在长期神经球培养中扩增的人类神经元干/祖细胞可促进蒙古沙鼠局灶性缺血后的功能恢复。

DOI：
发表时间：
2004
期刊：
Journal of Neuroscience Research 78
影响因子：
0
作者：
Ishibashi S;Sakaguchi M;Kuroiwa T;Yamasaki M;Kanemura Y;Ichinose S;Shimazaki T;Onodera M;Okano H;Mizusawa H.
通讯作者：
Mizusawa H.

A comparison of long-term neurological symptoms after two different focal ischemic models in Mongolian gerbils.

蒙古沙鼠两种不同局灶性缺血模型后长期神经症状的比较。