Segmentation during regeneration in annelids

环节动物再生过程中的分割

基本信息

批准号：
14596004
负责人：
TAKAHASHI Yoshiko
金额：
$ 2.3万
依托单位：
RIKEN (The Institute of Physical and Chemical Research)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14596004/
关键词：
annelids regeneration segmentation in situ hybridization cell proliferation Enchytraeus japonensis

项目摘要

Segmentation along the antero-posterior axis of the body is seen in a wide range of species in animal kingdom, including vertebrates, arthropods, and annelids. It remains unknown whether the mechanisms underlying the body segmentation are shared by these animals, or whether the segmental pattern emerged independently during animal evolution. Whereas the molecular mechanisms by which the segmentation is regulated have well been studied in Drosophila and vertebrates, those for annelids remain undetermined. We reasoned that the study of segmentation in annelids will provide valuable information concerning how the animals evolved their body pattern during evolution. We used Enchytraeus japonensis, a small worm recently found in Japan, which is endowed with a powerful capability of regeneration, to understand how their segmented body is established during regenerative processes. We identified cDNAs that were specifically expressed during the regeneration by subtractive hybridization technique, and also by in situ hybridization in histological sections. In addition, we, examined expression of genes known to be involved in the segmentation in Drosophila or vertebrates, such as Delta, Notch, and Wingless (Wnt). We have not found a segmented pattern expressed by these genes, implying that the segmentation in worms is regulated by distinct mechanisms from those in flies and vertebrates.

在动物界的许多物种中都可以看到沿身体前后轴的分割，包括脊椎动物、节肢动物和环节动物。目前尚不清楚这些动物是否共享身体分段的机制，或者分段模式是否在动物进化过程中独立出现。尽管在果蝇和脊椎动物中已经对调节分段的分子机制进行了深入研究，但环节动物的分子机制仍未确定。我们推断，对环节动物的分割研究将提供有关动物在进化过程中如何进化其身体模式的宝贵信息。我们利用最近在日本发现的一种具有强大再生能力的小蠕虫Enchytraeus japonensis来了解其分段身体在再生过程中是如何建立的。我们通过消减杂交技术以及组织学切片中的原位杂交鉴定了再生过程中特异性表达的 cDNA。此外，我们还检查了果蝇或脊椎动物中已知参与分割的基因的表达，例如 Delta、Notch 和 Wingless (Wnt)。我们还没有发现这些基因表达的分段模式，这意味着蠕虫的分段是通过与果蝇和脊椎动物不同的机制来调节的。