Studies on diversity and diversification process of instestinal flagellate communities of temites.

白蚁肠道鞭毛虫群落多样性及多样化过程的研究.

基本信息

批准号：
14596001
负责人：
KITADE Osamu
金额：
$ 2.3万
依托单位：
Ibaraki University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14596001/
关键词：
termites symbiosis protist diversity genetic variation parthenogenesis 共生微生物群集分子系統

项目摘要

1.Phylogeny reconstruction of Rhinotermitid host termitesPhylogeny of Rhinotermitid host termites, comprising of 14 genera, was inferred from DNA sequences (2120bps) of COI, COII and 16SrRNA genes using parsimony method.2.Comparison of phylogenies between host termites and symbiotic protistsSSUrRNA gene of Pseudotrichonympha spp. isolated from the Rhinotermitid hosts was sequenced and their phylogeny was reconstructed. Except for clones from one host species, the phylogenies of the hosts and the symbionts indicated coincidence of the topology, suggesting past co-speciation. Similar comparative analysis was carried out for Reticulitermes spp. (hosts) and Teranynpha spp. (symbionts) and the past cospeciation was also inferred except for clones from two host populations.3.Inference of evolution and diversification process of protist communityUsing character (presence/absence) mapping of each symbiont genus, past diversification of symbiont genera and host switchings were reconstructed on the phylogenetic tree of the Rhinotermitidae.4.Effect of host colony size on the diversity of symbiont communityFron the 3 months' monitoring of experimental termite colonies, effects of host colony size on the diversity of symbiont community was investigated. The decrease of colony size significantly reduced the species numbers and Shannon index of the community.5.Genetic diversity between symbiont individuals and populationVariation of SSUrRNA gene was investigated on Teranympha spp. from Reticulitermes hosts in and around Japan. In Yamaguchi population and Aichi population, sequence diversity were about 10 times higher than other populations. Gene clones from such populations were probably affected by lineage sorting caused by parthenogenesis of Teranympha.6.Mode of reproduction of host termitesThe mode of reproduction in Reticulitermes kanmonensis and R.speratus were investigated by field investigation and DNA genotyping. Inbreeding and fusion of field colonies were suggested.

1.鼻白蚁寄主白蚁的系统发育重建利用简约法从COI、COII和16SrRNA基因的DNA序列(2120bps)中推断出14个属的鼻白蚁寄主白蚁的系统发育。2.寄主白蚁与共生原生生物SSUrRNA基因的系统发育比较假毛线虫属对从鼻白蚁宿主中分离出来的病毒进行了测序并重建了它们的系统发育。除了来自一种宿主物种的克隆外，宿主和共生体的系统发育表明拓扑结构一致，表明过去的共物种形成。对散白蚁属进行了类似的比较分析。（宿主）和Teranynpha spp。（共生体）和过去的共种化也被推断出来，除了来自两个宿主种群的克隆。3.原生生物群落的进化和多样化过程的推断利用每个共生体属的特征（存在/不存在）映射，共生体属的过去的多样化和宿主转换重建鼻白蚁科系统发育树。4.宿主群体大小对鼻白蚁多样性的影响共生群落通过对实验白蚁群落进行3个月的监测，研究了宿主群落大小对共生群落多样性的影响。群体规模的减小显着降低了群落的物种数和香农指数。5.共生体个体和群体之间的遗传多样性对Teranympha spp.进行了SSUrRNA基因的变异研究。来自日本及其周边地区的散白蚁宿主。在山口种群和爱知种群中，序列多样性比其他种群高约10倍。这些群体的基因克隆可能受到白蚁单性生殖引起的谱系分选的影响。6.寄主白蚁的繁殖方式通过实地调查和DNA基因分型，对散白蚁和R.speratus的繁殖方式进行了研究。建议进行田间群体的近亲繁殖和融合。

项目成果

期刊论文数量（42）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Novel Glade of Rickettsia spp. from leeches.

立克次体属的小说空地。

DOI：
发表时间：
2002
期刊：
Applied and Environmental Microbiology 68
影响因子：
0
作者：
Kikuchi;Y.;Sameshima;S.;Kitade;O.;Kojima;J.;Fukatsu;T.
通讯作者：
T.

Hayashi, Y., Kitade, O., Kojima, J.: "Parthenogenetic repnduction in neotenics of the subterranean termite Reticulitermes speratus (Isoptera : Rhinotermitidae)"Entomological Science. 6巻4号. 253-257 (2003)

Hayashi, Y.、Kitade, O.、Kojima, J.：“地下白蚁 Reticulitermes speratus（等翅目：鼻白蚁科）的孤雌繁殖”《昆虫学科学》，第 6 卷，第 4 期，253-257（2003 年）。

DOI：
发表时间：
期刊：
影响因子：
0
作者：
通讯作者：

On the origin of termite workers : weighing up the phylogenetic evidence.

关于白蚁工蚁的起源：权衡系统发育证据。

DOI：
发表时间：
2004
期刊：
Journal of Evolutionary Biology 17
影响因子：
0
作者：
Thompson;G. et al.
通讯作者：
G. et al.

Molecular phylogeny of parabasalids inferred from small subunit rRNA sequenes, with emphasis on the Devescovinidae and Calonymphidae (Trichomonadea).

从小亚基 rRNA 序列推断出 parabasalids 的分子系统发育，重点是 Devescovinidae 和 Calonymphidae（毛滴虫科）。

DOI：
发表时间：
2002
期刊：
Molecular Phylogenetics and Evolution 25
影响因子：
0
作者：
Gerbod;D.;Noel;C.;Dolan;M.F.;Edgcomb;V.P.;Kitade;O.;Noda;S.;Dufernez;F.;Ohkuma;M.;Kudo;T.;Capron;M.;Sogin;M.;Viscogliosi;E.
通讯作者：
E.

Comparison of symbiotic flagellate faunae between termites and a wood-feeding cockroach of the genus Cryptocercus.