Analysis and application of newly discovered rat L1 retotransposon

新发现的大鼠L1转座子的分析及应用

• 批准号: 14580796
• 负责人: MORI Masayuki
• 金额: $ 2.37万
• 依托单位: Shinshu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14580796/
• 关键词: retrotransposon; rat; L1; mutation; Chediak-Higashi; Lyst

We have obtained the following data on L1b sequence found in the rat lysosomal trafficking regulator (Lyst) gene.1.Molecular genetic analysis of the Lyst gene of -ACI-beige rats, which is a model of human. Chediak-Higashi syndrome revealed deletion of exons 28,29,and 30 of the gene owing to recombination between L1 elements in the gene of the mutant rats. Nucleotide sequence analysis of one of the L1 elements (tentatively designated L1b) revealed that it had two intact at open reading frames (ORF1 and ORF2), a promotor-like sequence with Sp1 box in its 5' upstream region, a poly A sequence, in its 3' downstream region, and short direct repeats on up-and downstream region, which are hallmarks of active retrotransposons.2.The rat Lyst gene was mapped on the telomeric region of chromosome 17 by genetic linkage analysis utilizing (DA/Ham-Lyst^<bg> x BN)x DA/Ham-Lyst^<bg> baclcross rats.3.An allele-specific genotyping method for the Lyst gene, which enabled discrimination of the mutant Lyst^<bg>, Lyst^<bg-Kyo> alleles, and the normal Lyst allele, was developed. It was also demonstrated that the deletion break points in L1 elements were different between the Lyst^<bg> and Lyst^<bg-Kyo> alleles.4.Serial analysis gene expression (SAGE) analysis revealed that the transcription level of L1 in the mouse testis was very low.5.Retrotransposon activity was demonstrated in the assay utilizing HeLa cell culture assay.

我们已经获得了在大鼠溶酶体运输调节剂（LYST）基因中发现的L1B序列的以下数据。1。 -aci -Beige大鼠的LYST基因的分子遗传分析，这是人类的模型。 Chediak-Higashi综合征显示出外显子28,29的缺失，以及由于突变大鼠基因中的L1元素之间的重组而导致的30个基因。对L1元件之一（暂定指定的L1B）的核苷酸序列分析表明，它在开放式阅读框架（ORF1和ORF2）中具有两个完整的，这是一个具有5'上游区域中5'上游区域的SP1框的启动子样序列，一个poly A序列，在其3'下游区域，并在其下游区域中直接探讨了上游的元素，该区域是在上游的。通过利用（DA/HAM-LYST^<bg> X bn）X DA/HAM-LYST^<bg> baclcross大鼠的遗传连锁分析在17号染色​​体的端粒区域中映射。3。一种等位基因特异性基因分型方法，用于lyst基因，从而使lyst lyst lyst lyst^lyst^<bgg> lyst^<bg-lyers and l bg- <bg-开发。 It was also demonstrated that the deletion break points in L1 elements were different between the Lyst^<bg> and Lyst^<bg-Kyo> alleles.4.Serial analysis gene expression (SAGE) analysis revealed that the transcription level of L1 in the mouse testis was very low.5.Retrotransposon activity was demonstrated in the assay utilizing HeLa cell culture assay.

项目成果

Masui N, Nishikawa T, Takagi Y, Mori M, Suzuki T, Sato K.: "The rat lysosomal trafficking regulator (Lyst) gene is mapped on the telomeric region of chromosome 17."Experimental Animals. 52(1). 89-91 (2003)

Masui N、Nishikawa T、Takagi Y、Mori M、Suzuki T、Sato K.：“大鼠溶酶体运输调节因子 (Lyst) 基因定位在 17 号染色体的端粒区域。”实验动物。

Yao J, Chiba T, Sakai J, Hirose K, Yamamoto M, Hada A, Kuramoto K, Higuchi K, Mori M: "Mouse testis transcriptome revealed using serial analysis of gene expression."Mammalian Genome. (in press).

Yao J、Chiba T、Sakai J、Hirose K、Yamamoto M、Hada A、Kuramoto K、Higuchi K、Mori M：“利用基因表达的系列分析揭示小鼠睾丸转录组。”哺乳动物基因组。

Mori M, Yamasaki K, Nakanishi S, Kitada K, Higuchi K, Namiki C, Hamada S, Serikawa T.: "A new beige mutant rat ACI/N-Lyst^<bg-Kyo>."Experimental Animals. 52(1). 31-36 (2003)

Mori M、Yamasaki K、Nakanishi S、Kitada K、Higuchi K、Namiki C、Hamada S、Serikawa T.：“一种新的米色突变大鼠 ACI/N-Lyst^<bg-Kyo>。”实验动物。

Motoyuki Shimizu: "A congenic mouse and candidate gene at the chr 13 locus regulating bone density"Mammalian Genome. 13(7). 335-340 (2002)

Motoyuki Shimizu：“同类小鼠和调节骨密度的第 13 位基因座的候选基因”哺乳动物基因组。

Guo Z, Mori M, Fu X, Yao J, Xing Y, Korenaga T, Li G, Matsushita T, Hosokawa M, Higuchi K: "Amyloidosis modifier genes in less amyloidogenic A/J mouse strain."Laboratory Investigations. 83(11). 1605-1613 (2003)

Guo Z、Mori M、Fu X、Yao J、Xing Y、Korenaga T、Li G、Matsushita T、Hosokawa M、Higuchi K：“淀粉样变性较少的 A/J 小鼠品系中的淀粉样变修饰基因。”实验室研究。