The systematic survey for novel vasoactive peptides produced in the adrenal medulla.

肾上腺髓质产生的新型血管活性肽的系统调查。

• 批准号: 12470160
• 负责人: KITAMURA Kazuo
• 金额: $ 9.15万
• 依托单位: Miyazaki Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12470160/
• 关键词: adrenomedullin; PAMP; adrenomedullin (11-26); hypertensive peptide; Bovine adrenal medulla; radioimmunoassay; amidicin; C-terminal amide; PAMP; 新規生理活性ペプチド; アミド化; 生理活性ペプチド; 副腎髄質; 褐色細胞腫; 降圧ペプチド; ANP; BNP; CNP; 血小板cAMP増加作用; adrenomedullin; PAMP; adrenomedullin (11-26); hypertensive peptide; Bovine adrenal medulla; radioimmunoassay; amidicin; C-terminal amide; PAMP; 新規生理活性ペプチド; アミド化; 生理活性ペプチド; 副腎髄質; 褐色細胞腫; 降圧ペプチド; ANP; BNP; CNP; 血小板cAMP増加作用

Mammalian circulation is regulated by subtle mechanisms involving several neural and hormonal factors. Several vasoactive peptides including angiotensin, ANP and endothelin are important regulators in the cardiovascular system. For clarifying the intricacies of circulation control, it is important to discover still unidentified vasoactive peptide.We discovered adrenomedullin in 1993 and found another hypetensive peptide proadrenomedullin N-terminal 20 peptide (PAMP) which is produced from adrenomedullin precursor. In this study, we performed systematic survey for isopeptide of adrenomedullin and PAMP. Using the RIA for the ring structure of adrenomedullin, we isolated and determined adrenomedullin (11-26) as endogenous peptide. The synthetic adrenomedullin (11-26) produced dose-dependent strong pressor responses in unanesthetized rats in vivo.Using a novel method to detect a peptide with C-terminal amide structure, we isolated a novel 14 amino acids peptide where C-terminus was amidated. This peptide was termed amidicin. Amidicin is widely distributed in porcine tissue and is especially abundant in pituitary gland, cardiac ventricle and spleen. Amidicin may be a possible new peptide participating in circulation control.

哺乳动物的循环受涉及几种神经和激素因素的微妙机制调节。包括血管紧张素，ANP和内皮素在内的几种血管活性肽是心血管系统中重要的调节剂。为了阐明循环控制的复杂性，重要的是要发现仍然未识别的血管活性肽。我们在1993年发现了肾上腺素蛋白，并发现另一个hypection蛋白肽原肾上腺素甲肾上腺素N-末端20肽（PAMP）是由肾上腺素甲肾上腺素前体产生的。在这项研究中，我们对肾上腺甲基蛋白和PAMP的异肽进行了系统的调查。使用RIA作为肾上腺甲蛋白的环结构，我们将肾上腺甲蛋白（11-26）分离为内源性肽。合成肾上腺素蛋白（11-26）在体内未消除的大鼠中产生了剂量依赖性的强压反应。使用一种新型方法来检测具有C末端酰胺结构的肽，我们分离了一种新颖的14个氨基酸肽，其中分离了C-末端，其中C-末端被分合了C-末端。该肽被称为酰胺素。酰胺素广泛分布在猪组织中，垂体，心室和脾脏特别丰富。氨基蛋白可能是参与循环控制的新肽。