Functional and pathphysiological study in serotonin-containing chemorecptor cells

含血清素化学感受器细胞的功能和病理生理学研究

• 批准号: 12460128
• 负责人: ITO Shigeo
• 金额: $ 9.09万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12460128/
• 关键词: chicken thoracic aorta; 5-hydroxytryptamine; aortic body; voltage-gated channels; aminetransporter; nicotinic ACh receptor; 5-HT release; amine-containing cells; 5-HT再取込阻害薬; アンフェタミン誘導体; 細胞内Ca増加; 副腎髄質細胞; セロトニン; 腸クロム親和性細胞; ニコチン様アセチルコリン受容体; ホー; chicken thoracic aorta; 5-hydroxytryptamine; aortic body; voltage-gated channels; aminetransporter; nicotinic ACh receptor; 5-HT release; amine-containing cells; 5-HT再取込阻害薬; アンフェタミン誘導体; 細胞内Ca増加; 副腎髄質細胞; セロトニン; 腸クロム親和性細胞; ニコチン様アセチルコリン受容体; ホー

Serotonin (5-HT) -containing chemoreceptor cells were characterized using a whole-cell patch clamp technique. (1) 5-HT-containing chemoreceptor cells isolated from chicken thoracic aorta have voltage-gated Na^+ channels, L- and N-type Ca^<2+> channels and K^+ channels including oxygen-sensitive K^+ channels. (2) α-Bungarotoxin-sensitive and insensitive nicotinic ACh receptors were present in these cells. (3) Although we tried to isolate epitherial cells containing 5-HT (neutral red-positive cells) from the guinea-pig and rat intestinal mucosae, we could not obtain the cells responding to voltage-pulses under the voltage-clamp condition. (4) Excess KCl and veratridine evoked 5-HT output from the chicken thoracic aorta, which was inhibited by Ca^<2+> channel antagonists. ACh, nicotine and DMPP also evoked 5-HT output partly inhibited by α-bungarotoxin. (5) p-Chloroamphetamine (PCA) evoked time- and concentration-dependent 5-HT output from the aortic tissues. 5-HT reuptake inhibitors, flu … More voxamine, fluoxetine and clomipramine(CLO) caused a dose-dependent increase in 5-HT in the incubation medium, which were insensitive to extracellular Ca^<2+> removal. (6) CLO and PCA caused a temperature-dependent 5-HT output. CLO-induced 5-HT output was inhibited by extracellular Na^+ removal but not PCA-induced one. (7) 5-HT evoked oscillatory changes in tension and intracellular Ca^<2+> concentration ([Ca^<2+>]i) in the rat basilar artery. In vascular smooth muscles cells isolated from the rat basilar artery, UTP elicited increases in [Ca^<2+>]i in all cells examined, but 5-HT hardly evoked the response. (8) In PC12 cells, a vesicular amine uptake inhibitor, reserpine, decreased the content of dopamine but not ATP in the secretory vesicles. A vesicular H^+-pump ATPase inhibitor, bafilomycine A1, decreased both contents. (9) In adrenal chromaffin cells, both muscarine and caffeine elicited Ca^<2+>-activated K^+ currents concomitant with increases in [Ca^<2+>]i, but caffeine was more effective in eleciting K^+ currents even when both drugs evoked the increase of [Ca^<2+>]i to the same extent. Opioid agonists inhibited voltage-gated Ca^<2+> channels through G proteins. Less

使用全细胞贴片夹技术表征5-羟色胺（5-HT） - 含有化学感受器细胞。 （1）从鸡胸主动脉分离的5-HT化学感受器细胞具有电压门控的Na^+通道，L-和N型Ca^<2+>通道和K^+通道，包括氧气敏感的K^+通道。 （2）这些细胞中存在对α-核毒素敏感和不敏感的烟碱ACH受体。 （3）尽管我们试图从豚鼠和大鼠肠粘膜中分离含有5-HT（中性红阳性细胞）的层状细胞，但我们无法在电压钳状态下获得对电压脉冲响应的细胞。 （4）多余的KCl和veratridine引起了鸡胸主动脉的5-HT输出，该输出受到Ca^<2+>通道拮抗剂的抑制。 ACH，Nicotine和DMPP还诱发了5-HT输出，部分抑制了α-bungarotoxin。 （5）p-氯苯丙胺（PCA）诱发了来自主动脉组织的时间和浓度依赖性的5-HT输出。 5-HT再摄取抑制剂，流感……更多的雄性素，氟西汀和氯米帕明（CLO）在孵育培养基中导致5-HT的剂量依赖性增加，对细胞外Ca^<2+>切除不敏感。 （6）CLO和PCA导致温度依赖性5-HT输出。 CLO诱导的5-HT输出被细胞外Na^+去除而抑制，而不是PCA诱导的。 （7）5-HT诱发了大鼠基底动脉的张力和细胞内Ca^<2+>浓度的振荡变化。在从大鼠基底动脉分离的血管平滑肌细胞中，UTP在所有检查的细胞中引起[Ca^<2+>] I的增加，但5-HT几乎没有引起反应。 （8）在PC12细胞中，囊泡胺摄取抑制剂的伴侣会减少多巴胺的含量，但在秘密蔬菜中却不降低ATP。囊泡H^+ - 泵浦ATPase抑制剂Bafilomycine A1降低了这两个内容物。 （9）在肾上腺炎蛋白细胞中，毒arine和咖啡因都引起了Ca^<2+> - 激活的K^+电流，随着[Ca^<2+>] I的增加，咖啡因也更有效地在ELECITION K^+电流中，即使这两种药物都唤起了[Ca^^<2+>+ever的增加，咖啡因均在同一药物中增加。阿片类药物激动剂通过G蛋白抑制电压门控的Ca^<2+>通道。较少的