Functional and pathphysiological study in serotonin-containing chemorecptor cells

含血清素化学感受器细胞的功能和病理生理学研究

基本信息

批准号：
12460128
负责人：
ITO Shigeo
金额：
$ 9.09万
依托单位：
HOKKAIDO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

项目摘要

Serotonin (5-HT) -containing chemoreceptor cells were characterized using a whole-cell patch clamp technique. (1) 5-HT-containing chemoreceptor cells isolated from chicken thoracic aorta have voltage-gated Na^+ channels, L- and N-type Ca^<2+> channels and K^+ channels including oxygen-sensitive K^+ channels. (2) α-Bungarotoxin-sensitive and insensitive nicotinic ACh receptors were present in these cells. (3) Although we tried to isolate epitherial cells containing 5-HT (neutral red-positive cells) from the guinea-pig and rat intestinal mucosae, we could not obtain the cells responding to voltage-pulses under the voltage-clamp condition. (4) Excess KCl and veratridine evoked 5-HT output from the chicken thoracic aorta, which was inhibited by Ca^<2+> channel antagonists. ACh, nicotine and DMPP also evoked 5-HT output partly inhibited by α-bungarotoxin. (5) p-Chloroamphetamine (PCA) evoked time- and concentration-dependent 5-HT output from the aortic tissues. 5-HT reuptake inhibitors, flu … More voxamine, fluoxetine and clomipramine(CLO) caused a dose-dependent increase in 5-HT in the incubation medium, which were insensitive to extracellular Ca^<2+> removal. (6) CLO and PCA caused a temperature-dependent 5-HT output. CLO-induced 5-HT output was inhibited by extracellular Na^+ removal but not PCA-induced one. (7) 5-HT evoked oscillatory changes in tension and intracellular Ca^<2+> concentration ([Ca^<2+>]i) in the rat basilar artery. In vascular smooth muscles cells isolated from the rat basilar artery, UTP elicited increases in [Ca^<2+>]i in all cells examined, but 5-HT hardly evoked the response. (8) In PC12 cells, a vesicular amine uptake inhibitor, reserpine, decreased the content of dopamine but not ATP in the secretory vesicles. A vesicular H^+-pump ATPase inhibitor, bafilomycine A1, decreased both contents. (9) In adrenal chromaffin cells, both muscarine and caffeine elicited Ca^<2+>-activated K^+ currents concomitant with increases in [Ca^<2+>]i, but caffeine was more effective in eleciting K^+ currents even when both drugs evoked the increase of [Ca^<2+>]i to the same extent. Opioid agonists inhibited voltage-gated Ca^<2+> channels through G proteins. Less

5-羟色胺（5-HT）使用全细胞贴片夹技术的含化学感受器。在这些细胞中，含有氧气敏感的K^+通道CA^<2+>抑制α-甲状腺毒素的胸腔主动脉抑制了α-bungarotoxin。孵化培养基对细胞外Ca^<2+> clo的持续性和PCA的含量依赖于温度的5-HT输出。张力和细胞内Ca^<2+>在大鼠基底动脉中分离出的大鼠基底动脉中的张力。（8）在PC12细胞中，多巴胺的含量，但在分泌囊泡A囊泡H^+ - 泵浦ATPase抑制剂Bafilomycine A1中含量降低了两种含量（9）。 2+>激活的k^+电流随着[Ca^<2+>] I的增加，但咖啡因在电子中更多的k^+电流中的含量更多，从而避免了[Ca^<2+>] ioid激动剂的增加。 - 通过G蛋白少的Ca^<2 +>通道