Molecular basis for decline of leaf function during senescence: Analysis of major determinants causing decline of the amount of Rubisco
衰老过程中叶片功能下降的分子基础:Rubisco含量下降的主要决定因素分析
基本信息
- 批准号:12460028
- 负责人:
- 金额:$ 9.02万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (B)
- 财政年份:2000
- 资助国家:日本
- 起止时间:2000 至 2001
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
1) Rubisco was actively synthesized during expansion, but the amount of Rubisco synthesized rapidly declined just before full expansion. It already declined to about one fifth of the maximal amount at the time of fall expansion, and further declined to about one-tenth during senescence. The changes in the levels of rbcL and rbcS mRNAs were almost coordinated with those changes in the amount of Rubisco synthesized. Thus, the amount of Rubisco synthesized was primarily determined by the levels of rbcL and rbcS mRNAs throughout the life span of the leaves. Degradation of Rubisco became far more active than synthesis of Rubisco during senescence. Since the synthesis of Rubisco during senescence scarcely contributed to its amount, the degradation of Rubisco is the major determinant for the amount of Rubisco in the senescent leaves.2) The large subunit of Rubisco (LSU) was directly fragmented at Gly-329 into the 37-kDa and 16-kDa fragments by reactive oxygen species generated in the illuminated lysates of chloroplasts or intact chloroplasts. The LSU could also be cleaved into several other fragments by increasing the incubation time or the Fe^<2+> concentrations. All the cleavage sites were at or very close to the active site, just around the metal binding site within a radius of 12Å, indicating that proximity and favorable orientation are probably the most important parameter determining the cleavage sites. GS2 was also degraded by reactive oxygen species with the same manner as the LSU.
1)在扩张过程中,Rubisco合成活跃,但在完全扩张之前,Rubisco合成量迅速下降,已降至秋季扩张时最大量的五分之一左右,并在衰老过程中进一步下降至十分之一左右。 rbcL和rbcS mRNA水平的变化几乎与Rubisco合成量的变化一致,因此,Rubisco合成量主要由rbcL和rbcS水平决定。在叶子的整个生命周期中,Rubisco 的降解比衰老期间的 Rubisco 合成更加活跃,因为衰老过程中 Rubisco 的合成对其数量几乎没有贡献,因此 Rubisco 的降解是 Rubisco 数量的主要决定因素。 2) Rubisco (LSU) 大亚基在 Gly-329 处被产生的活性氧直接断裂成 37-kDa 和 16-kDa 片段。叶绿体或完整叶绿体的光照裂解物也可以通过增加孵育时间或Fe 2+ 浓度而被裂解成几个其他片段。所有裂解位点都位于或非常接近活性位点,就在活性位点周围。金属结合位点半径为 12Å,表明接近性和有利的方向可能是决定 GS2 也被具有相同活性氧的降解的最重要参数。方式与路易斯安那州立大学一样。
项目成果
期刊论文数量(70)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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MAE Tadahiko的其他文献
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{{ truncateString('MAE Tadahiko', 18)}}的其他基金
Molecular and physiological basis of Rubisco turnover and its relation to nitrogen nutrition
Rubisco转换的分子和生理基础及其与氮营养的关系
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15380046 - 财政年份:2003
- 资助金额:
$ 9.02万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Physiological and biochemical studies on leaf development and senescence : Degradation of Rubisco mediated by reactive oxygen species
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09460036 - 财政年份:1997
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APPLICATION OF TISSUE CULTURE TECHNIQUE TO THE DEVELOPMENT OF ACID-SOIL TOLERANT PLANT.
组织培养技术在耐酸性土壤植物培育中的应用。
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Molecular Basis for Nitrogen Remobilization with Leaf Senescence
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03454061 - 财政年份:1991
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$ 9.02万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
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