DNA Recombination of Thermophiles

嗜热菌 DNA 重组

• 批准号: 11694207
• 负责人: KURAMITSU Seiki
• 金额: $ 5.57万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11694207/
• 关键词: homologous recombination; DNA repair; extreme thermophile; hyperthermophile; archae; RecA; RadA; DNA; 遺伝子組換え; Thermus thermophilus HB8; Desulfurcoccus amyloliticus

In living organisms, genetic recombination is related to the DNA damage. DNA damage often arises as a result of genetic recombination, DNA replication, and other processes. In prokaryotes, about fifty enzymes participate in the DNA repair reactions. From thermophilic bacteria including hyperthermophile, we have cloned the DNA-repair related genes, which include recombinational repair enzymes (RecA, RuvA, RuvB, RuvC, ssb), base excision repair enzymes (MutM, MutY, EndoIII, EndoV), nucleotide excision repair enzymes (UvrA, UvrB, UvrC, UvrD), mismatch repair-enzymes (MutS, MutL), photolyase, and DNA polymerase I and ligase. Most of these DNA repair systems are common to many organisms, including those of Homo sapiens. These thermophilic enzymes, as expected, were stable. It was also found that the domains of the respective proteins can be easily isolated by partial digestion with protease. Some repair enzymes, including MutM, UvrB, and photolyase, have been crystallized and their three-dimensional structures have been determined. These thermophilic enzymes have been adapted to high temperatures. Some hyperthermophilic repair enzymes dramatically changed its conformation and increased the activity above 75℃

在生物体中，遗传重组与DNA损伤有关。 ，Endov），核苷酸切除酶（UVRA，UVRB，UVRC，UVRD），不匹配的修复酶（MUTS，MUTL）和DNA聚合酶I和连接酶，包括这些热蛋白酶。稳定。还可以通过蛋白质降低蛋白质的结构，并通过蛋白质消化酶急剧改变了其在75℃以上的TY中更改

项目成果

Sugahara, M.: "Crystal Structure of a Repair Enzyme of Oxidatively-Damaged-DNA, MutM (Fpg), from an Extreme Thermophile, Thermus thermophilus HB8"EMBO J.. 19 (15). 3857-3869 (2000)

Sugahara, M.：“来自极端嗜热菌、嗜热栖热菌 HB8 的氧化损伤 DNA 修复酶 MutM (Fpg) 的晶体结构”EMBO J.. 19 (15)。

Sugahara,M.: "Crystal Structure of a Repair Enzyme of Oxidatively-Damaged-DNA, MutM (Fpg), from an Extreme Thermophile, Thermus thermophilus HB8"EMBO J.. 19(15). 3857-3869 (2000)

Sugahara,M.：“氧化损伤 DNA 修复酶 MutM (Fpg) 的晶体结构，来自极端嗜热菌，嗜热栖​​热菌 HB8”EMBO J.. 19(15)。

Nakagawa,N.: "Crystal Structure of Thermus thermophilus HB8 UvrB Protein,A Key Enzyme of Nucleotide Excision Repair"J.Biochem.. 126(6). 986-990 (1999)

Nakakawa,N.：“嗜热栖热菌 HB8 UvrB 蛋白的晶体结构，核苷酸切除修复的关键酶”J.Biochem.. 126(6)。

Isaev-Ivanov,V.V.: "Fluorescence and Excitation Escherichia coli RecA Protein Spectra Analyzed Separately for Tyrosine and Tryptophan Residues"Arch.Biochem.Biophys.. 376(1). 124-140 (2000)

Isaev-Ivanov，V.V.：“分别分析酪氨酸和色氨酸残基的荧光和激发大肠杆菌 RecA 蛋白光谱”Arch.Biochem.Biophys.. 376(1)。

Sugahara,M.: "Crystal Structure of a Repair Enzyme of Oxidatively-Damaged-DNA,MutM (Fpg) , from an Extreme Thermophile, Thermus thermophilus HB8"EMBO J.. 19(15). 3857-3869 (2000)

Sugahara,M.：“氧化损伤 DNA 修复酶 MutM (Fpg) 的晶体结构，来自极端嗜热菌，嗜热栖​​热菌 HB8”EMBO J.. 19(15)。