Joint studies on the nuclear-encoded photosystem genes.

核编码光系统基因的联合研究。

基本信息

批准号：
11694190
负责人：
OBOKATA Junichi
金额：
$ 3.65万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

项目摘要

This research project was planned for promoting the international joint studies by the plant physiologists of Japan (five scientists mentioned above), US (Prof. Anastasios Melis, University of California, Berkeley), and Germany (Prof.Ralf Oelmueller, Friedrich-Schiller Universitaet, Jena) on the biogenesis of photosystem complexes in higher plants and algae. During the period supported by the grant, 21 scientists in total have visited the laboratories one another and stayed there for the total days of 148, and performed the cooperative studies. The outcomes of these research are 26 papers published in the journals and books, 17 presentations in the international meetings, and 29 presentations at domestic meetings, and two patent applications. The major scientific findings are as follows ;1, We found that plant photosystem genes have unique promoter architecture and transcription initiation mechanisms for the first time ;2, We showed that the biogenesis of photosystem complexes is subjected to positive feedback regulation by photosynthetic electron flow ;3, The redox regulation of photosystem genes operates not only on mRNA abundance but also on poly-ribosome loading ;4, We developed a set of special LED light sources that excite PSI and PSII alternatively ;5, We revealed that the abundance of chlorophyll b is not so important for the biogenesis of light-harvesting antennas ;6, CAO (chlorophyll-oxygenase) plays important roles in adjusting the antennae size to environmental light strength ;7, We proposed a quite new hypothesis to explain the origin and evolution of chloroplats, based on the phylogenetic analyzes of CAO genes.

该研究项目计划由日本工厂生理学家（上面提到的五名科学家），美国（Anastasios Melis教授，加利福尼亚大学，伯克利分校）和德国（弗里德里希·斯基勒大学教授，弗里德里希·斯基勒大学教授）计划推广国际联合研究。，Jena）关于高等植物和藻类光系统复合物的生物发生。在赠款支持的期间，总共21位科学家互相访问了实验室，并在那里呆了148天，并进行了合作研究。这些研究的结果是在期刊和书籍上发表的26篇论文，国际会议上的17个演讲，以及在国内会议上的29篇演讲，以及两项专利申请。主要的科学发现如下； 1，我们发现植物光系统基因首次具有独特的启动子结构和转录启动机制； 2，我们表明，光系统复合物的生物发生受光合电子流的积极反馈调节； 3，光系统基因的氧化还原调节不仅在mRNA丰度上运作，而且在多核糖体负载上运作； 4，我们开发了一组特殊的LED光源，使PSI和PSII激发了PSI和PSII； 5，我们揭示了叶绿素B的丰度对于轻度收获天线的生物发生并不重要； 6，CAO（叶绿素 - 氧合酶）在调整触角大小至环境光强度方面起着重要作用； 7，我们提出了一个相当新的假设来解释叶绿素的起源和进化，基于CAO基因的系统发育分析。