Molecular and morphological analyzes of floral organs in an asparagus homeotic mutant

芦笋同源异型突变体花器官的分子和形态学分析

基本信息

批准号：
15380018
负责人：
MASUDA Kiyoshi
金额：
$ 9.34万
依托单位：
Hokkaido University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15380018/
关键词：
asparagus homeotic mutant modified ABC model MADS-box gene Gold Schatz carpel 改変ABCモデル

项目摘要

Two vegetative lines of 'Gold Schatz' asparagus were introduced to Japan from the East Germany in 1966 and maintained in Hokkaido University through a vegetative propagation. One of these lines developed flowers with small tepals showing green color. Stamens were not found, but floral organs resembling the carpel differentiated at whorl 3 (stamen whorl). These adventitious carpels differentiated ovules including an embryo sac and egg cell. These morphological features suggested that the 'Gold Schatz' line might be a homeotic mutant of floral organogenesis, caused by a functional loss of class-B MADS-box genes.Southern blot analysis indicated that there was no difference in banding patterns of DNA fragments between wild type and mutant plants, when probed with DNA fragments specific for each of class-B and class-C MADS-box genes. Then, the expression of the MADS-box genes (AOAG1 and AOAG2 for class-C and AODEF, AOGLOA and AOGLOB for class-B genes) in wild type and mutant flowers was examined by Northern blot analysis. As a result, the expression of the B-functional genes were detected only in the wild type or scarcely detected in the mutant flowers. The expression of C-functional gene was detected in flowers from both lines. The hybridization experiments in situ displayd the exclusive expression of AODEF and AOGLOB at the primordia for inner tepals and stamens in wild type flowers. It appeared to remain during the early developmental stage, while the expression of AODEF in the mutant flowers rapidly disappeared in the early developmental stage. The transcripts for AOGLOA were not detected in the mutant flowers throughout the development.These results suggest that the lowered expression of AOGLOA causes a decline of the expression of AODEF and AOGLOB genes, and that the inability of B function genes leads to the phenotype found in an asparagus 'Gold Schatz' homeotic mutant.

1966年，从东德引入了两条“黄金沙茨”芦笋的营养线条，并通过营养繁殖在北海道大学维持。这些线条中的一条形成了带有小型绿色的花朵。找不到雄蕊，但是类似于螺纹的皮层的花器官在螺纹3（雄蕊螺旋）上有所区分。这些不定的皮层区分了包括胚囊和鸡蛋细胞在内的胚珠。这些形态学特征表明，“金schatz”系可能是花卉器官发生的同源突变体，这是由B类MADS-Box基因的功能丧失引起的。Southern印迹分析表明，当野生型和突变植物之间的DNA片段的带状模式在野生型和突变植物之间没有差异，当时是针对每个DNA片段的DNA片段，该dna trip priects of Class-b box-bbox class-b ands-b ands-b ands-c Mads-cand-c-cand-c-cand-c-card-c-card-c-card-c Mads-c-cans-cans cenes noce bects nation of。然后，通过Northern blot分析检查了野生型和突变花中MADS-Box基因（用于C类和AODEF，AOGLOA和AOGLOB的AOAG1和AOAG2）的表达。结果，仅在野生型中检测到B官能基因的表达或在突变花中几乎无法检测到。从两条线的花中检测到C官能基因的表达。原位杂交实验在野生型花中的内部（tepals and雄蕊）的原位显示了Aodef和Aoglob的独家表达。它似乎在早期发育阶段保留，而在突变花中AODEF的表达在早期发育阶段迅速消失。在整个发育过程中，在突变花中未检测到Aogloa的转录本。这些结果表明，Aogloa的表达降低会导致AODEF和AOGLOB基因的表达下降，并且B功能基因的无能导致在芦笋的Gold Schatz'Gold Schatz的Homeotic Syotic突变中发现的表型。

项目成果

期刊论文数量（24）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

J.-H.Park et al.: "Expression of AODEF, a B-functional MADS-box gene, in stamens and inner tepals of dioecious species Asparagus officinalis L."Plant Cell Physiol.. (in press).

J.-H.Park 等人：“AODEF（一种 B 功能性 MADS-box 基因）在雌雄异株芦笋种的雄蕊和内花被片中的表达”Plant Cell Physiol..（出版中）。

DOI：
发表时间：
期刊：
影响因子：
0
作者：
通讯作者：

The AVAG1 gene is involved in the development of the reproductive organs in the ornamental asparagus, Asparagus virgatus.

AVAG1 基因参与观赏芦笋 (Asparagus virgatus) 生殖器官的发育。

DOI：
发表时间：
2004
期刊：
Sexual Plant Reproduction 17
影响因子：
0
作者：
Jan;A.;et al.;P.-Y.Yun et al.
通讯作者：
P.-Y.Yun et al.

Molecular phylogeny of the genus Asparagus (Asparagaceae).

芦笋属（天冬酰胺科）的分子系统发育。

DOI：
发表时间：
期刊：
Plant Species Biology (in press)
影响因子：
0
作者：
Matsumoto H.(分担執筆);小瀬真吾;T.Fukuda et al.
通讯作者：
T.Fukuda et al.

Two GLOBOSA-like genes are expressed in second and third whorls of homochlamydeous flowers in Asparagus officinalis L.

DOI：
10.1093/pcp/pch040
发表时间：
2004-03-01
期刊：
PLANT AND CELL PHYSIOLOGY
影响因子：
4.9
作者：
Park, JH;Ishikawa, Y;Kameya, T
通讯作者：
Kameya, T

The differentiation of sepal and petal morphologies in Commelinaceae.

鸭跖草科萼片和花瓣形态的分化。

DOI：
发表时间：
2004
期刊：
Gene 343
影响因子：
0
作者：
San-oh;Y.;大川泰一郎;M.Hirai;A.Kanno;S.Mori;A.Kanno;S.Mori;M.Hirai;A.Kanno;S.Mori;M.Nakada;M.Nakano;M.Nakano;M.Nakano;Y.Adachi;S.Mori;T.Nakamura;T.Nakamura;S.Mori;T.Nakamura;S.Mori;T.Ito;T.Nakamura;Y.Adachi;S.Mori;S.Mori;T.Ochiai
通讯作者：
T.Ochiai