The genetic correlation between synapse formation and memory

突触形成与记忆之间的遗传相关性

基本信息

批准号：
14380374
负责人：
SAITOE Minoru
金额：
$ 8.64万
依托单位：
Tokyo Metropolitan Organization for Medical Research
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14380374/
关键词：
Drosophila syntaxin synapse formation spontaneous vesicle release syntaxin(syx)syntaxin cAMP CaMKII Gene Chip

项目摘要

The syntaxin (syx) gene encodes a synaptic plasma membrane protein that regulates synaptic vesicle release in association with synaptic vesicle protein such as synaptotagmin and synaptobrevin. In syx mutants, spontaneous vesicle release is absent, and postsynaptic receptor clusters are scarcely formed. We have hypothesized that synaptic vesicle contains clustering agent, and spontaneous vesicle release is sufficient to induce postsynaptic receptor clusters. In order to examine the role of spontaneous vesicle exocytosis more precisely, we analyze the developmental changes of synapse function and morphology in syx mutant embryos. At the early stage of synapse formation, we found spontaneous vesicle release in a quite low frequency manner suggesting contribution of maternal Syx protein. In consistent with this finding, synaptic receptor clusters were rarely observed. However, the ratio of the receptor cluster to synaptic area and intensity of clusters were significantly reduced in syx mutants. Furthermore, we found presynaptic defects in syx mutants. Number of synaptic branches were decreased, synaptic boutons swelled and less developed. These results suggest that maternal Syx protein contributes to residual spontaneous vesicle release at the early stage of synapse formation and Leads to rarely observed receptor clusters. syx regulates development of presynaptic terminals as well as synaptic vesicle release.

Syntaxin (syx) 基因编码突触质膜蛋白，该蛋白与突触结合蛋白和突触短蛋白等突触小泡蛋白相关，调节突触小泡的释放。在 syx 突变体中，不存在自发性囊泡释放，并且几乎不形成突触后受体簇。我们假设突触小泡含有聚集剂，自发的小泡释放足以诱导突触后受体簇。为了更准确地研究自发性囊泡胞吐作用的作用，我们分析了syx突变体胚胎中突触功能和形态的发育变化。在突触形成的早期阶段，我们发现自发性囊泡以相当低的频率释放，表明母体 Syx 蛋白的贡献。与这一发现一致的是，很少观察到突触受体簇。然而，在 syx 突变体中，受体簇与突触面积的比率和簇强度显着降低。此外，我们发现 syx 突变体存在突触前缺陷。突触分支数量减少，突触束肿胀且发育较差。这些结果表明，母体 Syx 蛋白有助于突触形成早期残留的自发囊泡释放，并导致很少观察到的受体簇。 syx 调节突触前末梢的发育以及突触小泡的释放。