Functional analysis of fatty acid binding proteins in immune and neural tissues.

免疫和神经组织中脂肪酸结合蛋白的功能分析。

基本信息

批准号：
14370002
负责人：
KONDO Hisatake
金额：
$ 8.83万
依托单位：
Tohoku University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14370002/
关键词：
fatty acid binding protein epidermal water barrier radial glia astrocyte thymic epithclial cell interleukine 12 dendritic cell 脂肪酸結合蛋白ラジアルグリア E-FABP B-FABP H-FABP 遺伝子欠損 DHA 記憶低下

项目摘要

Among fatty acid binding proteins (FABPs), the localization of brain(B)-and epidermal (E)-FABPs were examined in details in varous organs/tissues of mice, and gene knockout mice for the two FABPs were generated and their phenotypes were analysed in neuro-immune systems. While B-FABP was localized in the ventricular germinal zone and radial glial cells at prenatal stages and confined to the astrocytes in postnatal brain, E-FABP was localized in neurons and glia at the perinatal stage and confined to astrocytes in postnatal brain. While B-FABP was localized only in hepatic Kupffer cells, E-FABP was localized in splenic dendritic cells, peritoneal and alveolar macrophages, and thymic epithelical cells of adult mice. With the new advantage of E-FABP as a specific marker for the dendritc cells, the phagocytosis of lipopolysaccharide-induced apoptotic splenocytes in situ by the dendritic cells was first revealed. The basal transepidermal water loss of homozygous mice for E-FABP was lower than that of the wild mice. When the water permeability barrier of skin was disrupted by aceton application, recovery in transepidermal water loss was delayed, although no marked differences ware detected in ultrastructure and lipid contents of epidermis. Dendritic cells isolated from E-FABP-homozygous mice showed a marked decrease of IL-12 secretion. B-FABP-homozygous mice showed deterioration of learnining, memory anxiety.

在脂肪酸结合蛋白（FABP）中，详细检查了脑（B）-和表皮（E）-FABP在小鼠各个器官/组织中的定位，并生成了两种FABP的基因敲除小鼠，并对其表型进行了研究。在神经免疫系统中进行分析。 B-FABP 在产前阶段定位于心室生发区和放射状胶质细胞，在出生后大脑中局限于星形胶质细胞，而 E-FABP 在围产期阶段定位于神经元和神经胶质细胞，在出生后大脑中局限于星形胶质细胞。 B-FABP 仅定位于肝库普弗细胞，而 E-FABP 定位于成年小鼠的脾树突状细胞、腹膜和肺泡巨噬细胞以及胸腺上皮细胞。利用E-FABP作为树突状细胞特异性标记物的新优势，首次揭示了树突状细胞原位吞噬脂多糖诱导的脾细胞凋亡。 E-FABP纯合小鼠的基础经皮失水量低于野生小鼠。当皮肤的透水性屏障被丙酮破坏时，经表皮失水的恢复被延迟，尽管表皮的超微结构和脂质含量没有检测到显着差异。从 E-FABP 纯合小鼠中分离的树突状细胞显示 IL-12 分泌显着减少。 B-FABP纯合子小鼠表现出学习恶化、记忆焦虑。