Functional analysis of fatty acid binding proteins in immune and neural tissues.

免疫和神经组织中脂肪酸结合蛋白的功能分析。

基本信息

批准号：
14370002
负责人：
KONDO Hisatake
金额：
$ 8.83万
依托单位：
Tohoku University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14370002/
关键词：
fatty acid binding protein epidermal water barrier radial glia astrocyte thymic epithclial cell interleukine 12 dendritic cell 脂肪酸結合蛋白ラジアルグリア E-FABP B-FABP H-FABP 遺伝子欠損 DHA 記憶低下

项目摘要

Among fatty acid binding proteins (FABPs), the localization of brain(B)-and epidermal (E)-FABPs were examined in details in varous organs/tissues of mice, and gene knockout mice for the two FABPs were generated and their phenotypes were analysed in neuro-immune systems. While B-FABP was localized in the ventricular germinal zone and radial glial cells at prenatal stages and confined to the astrocytes in postnatal brain, E-FABP was localized in neurons and glia at the perinatal stage and confined to astrocytes in postnatal brain. While B-FABP was localized only in hepatic Kupffer cells, E-FABP was localized in splenic dendritic cells, peritoneal and alveolar macrophages, and thymic epithelical cells of adult mice. With the new advantage of E-FABP as a specific marker for the dendritc cells, the phagocytosis of lipopolysaccharide-induced apoptotic splenocytes in situ by the dendritic cells was first revealed. The basal transepidermal water loss of homozygous mice for E-FABP was lower than that of the wild mice. When the water permeability barrier of skin was disrupted by aceton application, recovery in transepidermal water loss was delayed, although no marked differences ware detected in ultrastructure and lipid contents of epidermis. Dendritic cells isolated from E-FABP-homozygous mice showed a marked decrease of IL-12 secretion. B-FABP-homozygous mice showed deterioration of learnining, memory anxiety.

在脂肪酸结合蛋白（FABPS）中，在小鼠的变异器官/组织中细节检查了大脑（B）的定位（B）和表皮（E） - fabps，并在Neuro-Mummune Systems中分析了两个Fabps的基因敲除小鼠的基因敲除小鼠。尽管B-FABP位于产前阶段的心室生发区域和径向神经胶质细胞中，并局限于产后大脑的星形胶质细胞，但E-FABP位于围产期阶段的神经元和神经胶质中，并局限于后大脑的星形胶质细胞。虽然B-FABP仅位于肝库普弗细胞中，但E-FABP位于脾脏树突状细胞，腹膜和肺泡巨噬细胞以及成年小鼠的胸腺上皮细胞中。借助E-FABP作为Dendritc细胞的特异性标记，首先揭示了树突状细胞原位脂多糖诱导的凋亡脾脏的吞噬作用。 E-FABP纯合小鼠的基础跨甲状腺皮质损失低于野生小鼠。当施用Aceton的渗透性破坏皮肤的水渗透性障碍时，延迟旋转皮肤水分流失的恢复，尽管在超微结构和表皮的脂质含量中未检测到明显的差异。从E-FABP-双生小鼠中分离出的树突状细胞显示IL-12分泌显着降低。 B-Fabp-Homozygous小鼠表现出学习，记忆焦虑的恶化。