Production of a chicken resistant strain using antiviral Mx gene

利用抗病毒 Mx 基因生产鸡抗性品系

基本信息

批准号：
14360161
负责人：
WATANABE Tomomasa
金额：
$ 8.58万
依托单位：
HOKKAIDO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

项目摘要

We have reported the polymorphism of antiviral Mx gene in the chicken, and that the specific amino acid substitution at position 631 corresponds to the differential antiviral activity. Namely, the chicken Mx gene is a resistant character in the case of Ash at the position 631 against the infectious experiment using influenza virus and vesicular stomatitis virus, and is sensitive in the case of Ser. In this study, we have established the simple method of genetic diagnosis whether each chicken carry the resistant or sensitive Mx gene by PCR-RLFP (restriction enzyme length fragment polymorphism) using genomic DNA extracted from one-drop of blood. As the result of determination in 500 indivuduals of the chicken commercial breeds, about 50% of them carry the resistant Mx gene and also about 50% have the sensitive Mx gene. In addition, it was demonstrated by the nucleotide sequence analysis of last exon that the Mx gene from only a jungle fowl (red jungle fowl) coded the resistant type of Ash, but those from the all other jungle fowl (red, gray and green jungle fowl) did the sensitive type of Ser. Furthermore, another birds (Galliformes) such as Japanese quail and and turkey carry the sensitive type of Ser. Therefore, from these results, the sensitive Mx gene was considered as the ancestor type in the bird, and the resistant Mx gene might appear with domestication of chicken.

我们报道了鸡抗病毒Mx基因的多态性，并且631位的特定氨基酸替换对应于差异的抗病毒活性。即，鸡Mx基因在631位的Ash的情况下是对使用流感病毒和水泡性口炎病毒的感染实验的抗性特征，而在Ser的情况下是敏感的。在本研究中，我们利用从一滴血液中提取的基因组DNA，通过PCR-RLFP（限制性酶切长度片段多态性）建立了简单的基因诊断方法，对每只鸡是否携带耐药或敏感Mx基因。对500只商业鸡种的测定结果，约50%携带抗性Mx基因，约50%携带敏感Mx基因。另外，通过最后一个外显子的核苷酸序列分析表明，只有原鸡（红原鸡）的Mx基因编码了Ash的抗性类型，而所有其他原鸡（红原鸡、灰原鸡和绿原鸡）的Mx基因都编码了抗性型Ash。鸡）做了敏感类型的 Ser。此外，另一种鸟类（鸡形目），例如日本鹌鹑和火鸡，也携带敏感型 Ser。因此，从这些结果来看，敏感的Mx基因被认为是鸟类的祖先类型，而抗性的Mx基因可能随着鸡的驯化而出现。

项目成果

期刊论文数量（56）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Nakatsu Y., Watanabe T.et al.: "Genetic polymorphisms and antiviral activirty in the bovine Mx1gene."Animal Genetics. (In press). (2004)

Nakatsu Y.、Watanabe T.et al.：“牛 Mx1 基因的遗传多态性和抗病毒活性。”动物遗传学。

DOI：
发表时间：
期刊：
影响因子：
0
作者：
通讯作者：

Genetic polymorphisms and antiviral acitivity in the bovine Mx gene.

牛 Mx 基因的遗传多态性和抗病毒活性。

DOI：
发表时间：
2004
期刊：
Animal Genetics 35
影响因子：
0
作者：
Nakatsu Y.;Yamada K.;Ueda J.;Onogi A.;Ables G.P.;Nishibori M.;Takada A.;Hata H.;Sawai K.;Tanabe Y.;Morita N.;Daikohara M;Watanabe T.
通讯作者：
Watanabe T.

EI-Nahas A., Watanabe T.et al.: "The differential effect of Kitw. mutation with other Kit allelic combinations on male sterility among inter-or intraspecific hybrid mice"Biochemical Genetics. 40. 13-25 (2002)

EI-Nahas A.、Watanabe T.等人：“Kitw. 突变与其他 Kit 等位基因组合对种间或种内杂交小鼠雄性不育的差异效应”生化遗传学。