Analysis of microbial metabolism of biosulfur compounds and adaptive evolution for the degradation of xenobiotics

生物硫化合物的微生物代谢分析和外源物质降解的适应性进化

• 批准号: 14360050
• 负责人: OMORI Toshio
• 金额: $ 8.06万
• 依托单位: Shibaura Institute of Technology (2003)The University of Tokyo (2002)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14360050/
• 关键词: Dimethyl Sulfide; Dibenzothiophene; Organic sulfur compounds; Dimethyl Sulfone; sigma54; Sulfate starvation; Dimethyl Slufide; Dimethyl Slufone; Dimethyl Sulfide; Dibenzothiophene; Organic sulfur compounds; Dimethyl Sulfone; sigma54; Sulfate starvation; Dimethyl Slufide; Dimethyl Slufone

Microbial dimethyl sulfide (DMS) conversion is thought to be involved in the global sulfur cycle. We isolated Pseudomonas putida strain DS1 from soil as a bacterium utilizing DMS as a sole sulfur source, and tried to elucidate the DMS conversion mechanism of strain DS1 at biochemical and genetic level. Strain DS1 oxidized DMS to dimethyl sulfone ( DMSO_2) via dimethyl sulfoxide, whereas the oxidation was repressed in the presence of sulfate, suggesting that a sulfate starvation response is involved in DMS utilization by starin DS1. Two of the five DMS-utilization-defective mutants isolated by transposon5 (Th5) mutagenesis had a Tn5 insertion in the ssuEAECBF operon, which has been reported to encode a two-component monooxygenase system (SsuED), an ABC-type transporter (SsuABC), and a small protein (SsuF), and also to play a key role in utilization of sulfonates and sulfate esters in another bacterium, P.pulida strain S-313. Disniption of ssuD and SsuD enzymatic activity demonstrated that methanesulfonate is a metabolic intermediate of DMS and desulfonated by SsuD.Disruption of ssuC cr ssuF also led to a DMS-utilization-defective phenotype. Another two mutants bad a defect in a gene homologous to pa2354 from P.aeruginosa PA01, which encodes a putative transcriptional regulator, while the remaining mutant had a defect in cysM encoding O-acetylserine(thio)-lyase B.

微生物二甲基硫化物（DMS）转化被认为与全球硫周期有关。我们从土壤中分离了pseudomonas putida菌株DS1作为一种利用DMS作为唯一硫源的细菌，并试图阐明在生化和遗传水平下DS1菌株DS1的DMS转化机制。菌株DS1通过二甲基亚氧化二甲基磺基氧化DMS（DMSO_2）通过硫酸盐存在下抑制氧化，这表明Starin DS1的DMS利用中涉及硫酸盐饥饿反应。通过转座子5（th5）诱变分离的五个DMS-利用缺陷的突变体中有两个在SSUEAECBF操纵子中插入TN5插入，据报道，该诱变量插入了两种成分单加友酶系统（SSUED），该系统（SSUED）是ABC-type Typorter（SSUABC）和SSSUABC（SSSUABC）和SS SSU的作用（以及SSSU的propent and sys and sys and stuf）（ssuuf）（ssuuf）（ssuuf）（ssuuf）;另一种细菌中的硫酸盐和硫酸盐酯S-313。 SSUD和SSUD酶促活性的脱离表明，甲烷磺酸盐是DMS的代谢中间体，并通过SSUD进行了脱硫。另外两个突变体在与P.Aeruginosa Pa01同源的基因中缺陷，该缺陷编码了推定的转录调节剂，而其余的突变体在编码O-乙酰梅碱（Thio）-lyase-lyase-B的Cysm中存在缺陷。