Bio-material pinning to the substrate and its evaluation

生物材料对基质的钉扎及其评估

基本信息

批准号：
14350366
负责人：
WADAYAMA Toshimasa
金额：
$ 9.22万
依托单位：
Tohoku University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

项目摘要

In this study, we aimed to establish a new method for pinning of sample molecules to the substrate surface and to detect faint signals due to the mono-layer sample with surface-enhanced vibrational spectroscopies. The results obtained are summarized as follows.1.Multiple IR ATR (attenuated total reflection) measurements indicate that hydrogen bond between the substrate"poly-vinyl alcohol (PVA)"and sample"adenosine"molecules. We have attempted to pinning adenosine molecules to the PVA thin film surface formed on the Si substrate by using a brass-made"transfer roller". Surface enhanced Raman spectral (SERS) study indicates that adenosine molecules can be pinned from the transfer roller onto the PVA surface through the hydrogen bond. The optimum condition for the pinning molecules to the substrate is yet to be elucidated.2.We have proposed another method for pinning of sample molecules, namely"pressure-attachment"(PA) method. In the PA method, Ag island films (5nm in thickness) are evaporated on glass plate and powder of the sample molecule is dispersed onto the Ag island films with spatula. After air-blowing excess powder, we applied pressure on the glass plate covered the molecule-dispersed Ag island films. Para-nitro benzoic acid (PNBA) was adsorbed on the Ag islands film surface through ionization and the SERS signals of ionized PNBA increased in intensity from the applying pressure of 0 to 0.6 MPa In contrast, the SERS signals due to adenosine adsorbed on the Ag islands surface remained nearly unchanged during applying pressure. The results obtained suggest that pinning of molecules by the PA method depends upon the affinity between the molecule and the substrate surface.

在这项研究中，我们的目的是建立一种将样品分子固定到基底表面的新方法，并通过表面增强振动光谱检测单层样品产生的微弱信号。所得结果总结如下： 1.多次IR ATR(衰减全反射)测量表明底物“聚乙烯醇(PVA)”与样品“腺苷”分子之间存在氢键。我们尝试通过使用黄铜制成的“转移辊”将腺苷分子钉扎到Si基底上形成的PVA薄膜表面。表面增强拉曼光谱 (SERS) 研究表明，腺苷分子可以通过氢键从转印辊固定到 PVA 表面。分子钉扎到基底上的最佳条件尚未阐明。2.我们提出了另一种钉扎样品分子的方法，即“压力附着”(PA)法。在PA方法中，将Ag岛膜（厚度5nm）蒸发在玻璃板上，然后用抹刀将样品分子的粉末分散到Ag岛膜上。吹掉多余的粉末后，我们对覆盖分子分散银岛膜的玻璃板施加压力。对硝基苯甲酸（PNBA）通过电离作用吸附在银岛膜表面，电离的 PNBA 的 SERS 信号强度从施加压力 0 到 0.6 MPa 增加，相反，由于腺苷吸附在银岛膜表面，SERS 信号强度增加。在施加压力期间，岛屿表面几乎保持不变。获得的结果表明，PA 方法对分子的钉扎取决于分子与基底表面之间的亲和力。