Bio-material pinning to the substrate and its evaluation

生物材料对基质的钉扎及其评估

基本信息

批准号：
14350366
负责人：
WADAYAMA Toshimasa
金额：
$ 9.22万
依托单位：
Tohoku University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

项目摘要

In this study, we aimed to establish a new method for pinning of sample molecules to the substrate surface and to detect faint signals due to the mono-layer sample with surface-enhanced vibrational spectroscopies. The results obtained are summarized as follows.1.Multiple IR ATR (attenuated total reflection) measurements indicate that hydrogen bond between the substrate"poly-vinyl alcohol (PVA)"and sample"adenosine"molecules. We have attempted to pinning adenosine molecules to the PVA thin film surface formed on the Si substrate by using a brass-made"transfer roller". Surface enhanced Raman spectral (SERS) study indicates that adenosine molecules can be pinned from the transfer roller onto the PVA surface through the hydrogen bond. The optimum condition for the pinning molecules to the substrate is yet to be elucidated.2.We have proposed another method for pinning of sample molecules, namely"pressure-attachment"(PA) method. In the PA method, Ag island films (5nm in thickness) are evaporated on glass plate and powder of the sample molecule is dispersed onto the Ag island films with spatula. After air-blowing excess powder, we applied pressure on the glass plate covered the molecule-dispersed Ag island films. Para-nitro benzoic acid (PNBA) was adsorbed on the Ag islands film surface through ionization and the SERS signals of ionized PNBA increased in intensity from the applying pressure of 0 to 0.6 MPa In contrast, the SERS signals due to adenosine adsorbed on the Ag islands surface remained nearly unchanged during applying pressure. The results obtained suggest that pinning of molecules by the PA method depends upon the affinity between the molecule and the substrate surface.

在这项研究中，我们旨在建立一种将样品分子固定到底物表面的新方法，并由于具有表面增强的振动光谱镜的单层样品而检测到微弱的信号。所获得的结果总结如下。1。多重IR ATR（衰减的总反射）测量表明，底物“聚乙烯基醇（PVA）”和样品“腺苷”分子之间的氢键。我们试图通过使用黄铜制成的“转移辊”将腺苷分子固定在SI基板上形成的PVA薄膜表面上。表面增强的拉曼光谱（SERS）研究表明，腺苷分子可以通过氢键从转移滚轮固定到PVA表面。将固定分子固定到底物的最佳条件尚待阐明。2。我们提出了另一种固定样品分子的方法，即“压力跟踪”（PA）方法。在PA方法中，将AG岛膜（厚度为5nm）蒸发在玻璃板上，样品分子的粉末分散到带有刮刀的Ag岛膜上。吹气过多的粉末后，我们在玻璃板上施加了压力，覆盖了分子分散的AG岛膜。帕拉硝基苯甲酸（PNBA）通过离子化在AG岛膜表面吸附，而电离PNBA的SERS信号的强度从0到0.6 MPa的施加压力增加，相比之下，由于在AG Islands表面在施加压力下几乎没有结合的腺苷引起的SERS信号，SERS信号几乎保持不变。获得的结果表明，通过PA方法将分子固定取决于分子和底物表面之间的亲和力。