The effect of treadnill running training on neural plasticity in the hippocampus in juverile rats.

跑步训练对幼年大鼠海马神经可塑性的影响。

基本信息

批准号：
11480004
负责人：
SOYA Hideaki
金额：
$ 9.02万
依托单位：
Institute of Health and Sports Sciences, University of Tsukuba
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2001
项目状态：
已结题

项目摘要

In this study, we investigated following three experiments. First, we examined whether acute running at the lactate threshold (LT) acts on the dentate gyms (DG) to induce BDNF mRNA expression, we used a running model using Wistar male rats (Experiment 1). Second, we examined whether chronic running training either or above the LT specifically causes up-regulation of BDNF mRNA in the DG (Experiment 2). Finally, we estimated how the same types of exercise affects neurogeniesis in DG. In the experiment 1, exercise induced BDNF mRNA expressions, but these expressions were not observed depending on exercise intensity. These results suggest that the hippocampus would be activated even by low exercise intensity in acute single bout of exercise.In the experiment 2, rats were subjected to Naive (N: sedentary), and to 4 weeks of running training either below-(4W: walking) or supra-(4T, trotting) LT The effects of these training on hippocampal BDNF mRNA and morris water maze were examined. Unlike … More the experiment 1, the BDNF mRNA levels in the DG were observed to be up-regulated in rats in 41 but not in 4W Since plasma corticosterone levels and adrenal weight in 4T were significantly higher than those in N, chronic exericse increased hippocampal neuroplastisity though stress response occurred concurrently. There were no significant differences in spatial learning in the morris water maze. Thus we failed to confirm the hippocampal neuroplastisity by behavioral approach.These results suggest that acute LT running has the potential role of inducing BDNF mRNA expression in the DG. Adaptive up-regulation of BDNF mRNA expression can be induced by light running below the LT in acute running, and also can be induced by high intensity running (supra-LT) in chronic running. In addition, chronic running tended to be induced neurogenesis (Experiment 3), indicating exercise training would be increased hippocampal plastisity.It is worth examining that exercise induced hippocampal plastisity can be detected by morris water maze again, or some other behavioral approaches. Less

在本研究中，我们研究了以下三个实验：首先，我们检查了乳酸阈值 (LT) 下的急性跑步是否作用于齿状肌 (DG) 来诱导 BDNF mRNA 表达，我们使用 Wistar 雄性大鼠作为跑步模型（实验 1）。其次，我们检查了长期跑步训练是否会导致 DG 中 BDNF mRNA 的上调（实验 2）。最后，我们估计了相同类型的运动如何影响神经发生。 DG.在实验1中，运动诱导BDNF mRNA表达，但这些表达并未根据运动强度观察到。这些结果表明，即使在急性单次运动中，海马体也会被激活。在实验2中，大鼠接受 Naive（N：久坐）和 4 周低于（4W：步行）或高于（4T，小跑）LT 的跑步训练。这些训练对海马 BDNF 的影响检查 mRNA 和莫里斯水迷宫，与实验 1 不同，观察到 41 组大鼠 DG 中的 BDNF mRNA 水平上调，但 4W 组大鼠没有上调，因为 4T 组大鼠的血浆皮质酮水平和肾上腺重量显着高于 4T 组大鼠。在N中，尽管应激反应同时发生，但长期运动增加了海马神经可塑性。莫里斯水迷宫中的空间学习没有显着差异，因此我们未能证实这一点。这些结果表明，急性LT跑步具有诱导DG中BDNF mRNA表达的潜在作用，在急性跑步中低于LT的光照可以诱导BDNF mRNA表达的适应性上调。慢性跑步中高强度跑步（supra-LT）会诱导神经发生（实验3），表明运动训练会增加海马。可塑性。值得研究的是，运动引起的海马可塑性可以再次通过莫里斯水迷宫或其他一些行为方法来检测。