The effect of treadnill running training on neural plasticity in the hippocampus in juverile rats.

跑步训练对幼年大鼠海马神经可塑性的影响。

基本信息

批准号：
11480004
负责人：
SOYA Hideaki
金额：
$ 9.02万
依托单位：
Institute of Health and Sports Sciences, University of Tsukuba
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2001
项目状态：
已结题

项目摘要

In this study, we investigated following three experiments. First, we examined whether acute running at the lactate threshold (LT) acts on the dentate gyms (DG) to induce BDNF mRNA expression, we used a running model using Wistar male rats (Experiment 1). Second, we examined whether chronic running training either or above the LT specifically causes up-regulation of BDNF mRNA in the DG (Experiment 2). Finally, we estimated how the same types of exercise affects neurogeniesis in DG. In the experiment 1, exercise induced BDNF mRNA expressions, but these expressions were not observed depending on exercise intensity. These results suggest that the hippocampus would be activated even by low exercise intensity in acute single bout of exercise.In the experiment 2, rats were subjected to Naive (N: sedentary), and to 4 weeks of running training either below-(4W: walking) or supra-(4T, trotting) LT The effects of these training on hippocampal BDNF mRNA and morris water maze were examined. Unlike … More the experiment 1, the BDNF mRNA levels in the DG were observed to be up-regulated in rats in 41 but not in 4W Since plasma corticosterone levels and adrenal weight in 4T were significantly higher than those in N, chronic exericse increased hippocampal neuroplastisity though stress response occurred concurrently. There were no significant differences in spatial learning in the morris water maze. Thus we failed to confirm the hippocampal neuroplastisity by behavioral approach.These results suggest that acute LT running has the potential role of inducing BDNF mRNA expression in the DG. Adaptive up-regulation of BDNF mRNA expression can be induced by light running below the LT in acute running, and also can be induced by high intensity running (supra-LT) in chronic running. In addition, chronic running tended to be induced neurogenesis (Experiment 3), indicating exercise training would be increased hippocampal plastisity.It is worth examining that exercise induced hippocampal plastisity can be detected by morris water maze again, or some other behavioral approaches. Less

在这项研究中，我们研究了三个实验。首先，我们检查了在鞋底阈值（LT）上运行的急性是否作用于牙齿体育馆（DG）以诱导BDNF mRNA表达，我们使用了使用Wistar雄性大鼠的跑步模型（实验1）。其次，我们检查了长期运行训练是否或以上是LT特异性引起DG中BDNF mRNA的上调（实验2）。最后，我们估计了相同类型的运动如何影响DG中的神经基因。在实验1中，运动诱导的BDNF mRNA表达式，但没有根据运动强度观察到这些表达。这些结果表明，即使在运动的急性单次回合中，也可以通过低运动强度来激活海马。在实验2中，大鼠接受幼稚（N：久坐），以及在低于 - （4W：步行）或SUPRA-（4T）或SUPRA-（4T，小跑）的训练下进行训练的4周，对这些训练对Hippocampal bdnfris water的影响进行了检查。与……更多的实验1不同，观察到DG中的BDNF mRNA水平在41中的大鼠中被上调，但在4W中没有上调，因为血浆皮质酮的水平和4T的肾上腺体重显着高于N的慢性锻炼，尽管慢性运动的慢性锻炼增加了，但在海马神经质量反应增加了，尽管在伴有杂种中会出现显着差异。我们未能通过行为方法来确认海马神经质质性。这些结果表明，急性LT运行具有诱导的BDNF mRNA表达在DG中的潜在作用。 BDNF mRNA表达的自适应上调可以通过在急性运行中LT以下奔跑的光引起，并且在慢性运行中也可以通过高强度运行（SUPRA-LT）引起。此外，慢性跑步倾向于引起神经发生（实验3），表明运动训练将增加海马塑性。值得研究的是，莫里斯水迷宫再次检测到运动诱导的海马塑性，或其他某些行为方法。较少的