Study on transcutaneous immunization using peptides of melanomd entigens with high affinity to MHC

MHC高亲和力黑色素抗原肽经皮免疫研究

• 批准号: 11470181
• 负责人: SAIDA Toshiaki
• 金额: $ 9.28万
• 依托单位: Shinshu University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11470181/
• 关键词: Malignant melnomd; Immunotherapy; Antigen peptide; Transcutaneous immunization; 抗原ペプチド; 経皮感作; 樹状細胞; 細胞障害性T細胞; Malignant melnomd; Immunotherapy; Antigen peptide; Transcutaneous immunization; 抗原ペプチド; 経皮感作; 樹状細胞; 細胞障害性T細胞

In our previous studey, we investigated induction of cytotoxic T lymphocytes ( CTL ) against melanoma cells by means of percutaneous application of epitope peptide of melanoma antigens. Application of mouse-gp100 peptide ( EGSRNQDWL ) but not human gp100 peptide ( KVPPNQDWL ) on tape-stripped skin of ear and abdomen of C57BL/6 mice induced slight immune response against B16 mouse melanoma, which was confirmed by CTL assay.This year, we investigated whether this treatment could induce delayed type hypersensitivity reaction ( DTH ) and inhibit growth of B16 melanoma in vivo. Application of the mouse-gp100 peptide on the tapestripped skin of the ear and abdomen of the mice induced DTH reaction, which was detected by foodpad test. However, growth of B16 melanoma was not significantly inhibited in the mice treated with the application of mouse-gp100 peptide.Seo et al reported strong inhibitory effect on the growth of B16 melanoma with almost the same treatment in this murine melanoma system. However, we could not confirm the excellent results in our study. Further study may be necessary to determine the effect of this unique method of immunization.

在我们以前的研究中，我们通过经皮施用了黑色素瘤抗原的表位肽对黑色素瘤细胞的细胞毒性T淋巴细胞（CTL）的诱导。在C57BL/6小鼠的耳朵和腹部的胶带皮肤皮肤和腹部上施用小鼠GP100肽（EGSRNQDWL），但不应用人类GP100肽（KVPPNQDWL）在胶带撕裂的皮肤上诱导了对B16小鼠黑色素瘤的轻微免疫反应，这是否可以通过ctl Assay确认，这是否可以延迟（我们调查了延迟）（诱导了延迟（在延迟）体内B16黑色素瘤的生长。在小鼠的耳朵和腹部腹部皮肤上，将小鼠GP100肽应用于诱导的DTH反应，该反应是通过食品板测试检测的。然而，在使用小鼠GP100肽治疗的小鼠中，B16黑色素瘤的生长并未受到显着抑制。Seo等报道，在该鼠类黑色素瘤系统中，对B16黑色素瘤生长的强烈抑制作用对B16黑色素瘤的生长产生了强烈的抑制作用。但是，我们无法在研究中确认出色的结果。可能需要进一步的研究来确定这种免疫接种方法的影响。