Cytological studies of the relationship between endocytosymbiosis and arrangement and configuration of organelles in algal cells with special emphasis on the evolutionary significance.

细胞学研究藻类细胞内细胞共生与细胞器排列和构型之间的关系，特别强调进化意义。

基本信息

批准号：
10440245
负责人：
HARA Yoshiaki
金额：
$ 4.35万
依托单位：
Faculty of Science, Yamagata University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B).
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2000
项目状态：
已结题

项目摘要

On the process of evolutionary endosymbiosis, configuration and structure of organelles in the host cell have been turburated by the invation of symbionts. After that, these organelles together with symbiont's ones were rearranged for a novel cell under the genetical and physiological regulation of the host cell. In particular, subcellular diversity found in higher taxa can be considered to be caused by these tuburance and rearrangement of organelles in each cell which was established by endocytosymbiosis. It is not so difficult to assume that these phenomina commonly give rise among phylogenetically different taxa.Using unicellular red algae belonging to the Porphyridiales, Rhodophyta, their subcellular diversity were examined on the focus of their organelles such as chloroplast-pyrenoid and nucleus stained by fluoresent dye under conforcal laser microscope and fluorescent microscopes.As a result, we can distinguished a seies of configurational clain or stepwise morphologies on their … More organellar sturucutres as followings ; 1) photosynthetic organelles increasing their volum and expanding thier surface and a nucleus located not in the center of a cell, 2) embedded pyrenoids changing into naked ones, and chloroplast body showing tendency from central part of cell to the periphery, 3) pyrenoid and nucleus showing the tendency frm cell periphery to the central part of cell, 4) they sometimes compete to occupy the central part of cell with each other, however, nucleus finally occupy the central part of cell, 5) the pyrenoid pushed away from the central part of cell disappearing and chloroplast located cell periphery changing its number in a cell from single to multiple. These results supprted the systematics based on their ultrastructure, habitat, types of the asexual reproduction and composition of phytosynthetic pigments published by Hara and Chihara (1992). In contrast, they could not support the molecular phylogenetic relationship examined by Hara, Yokoyama and Kim (2000) based on their 18SrDNA and psbA Less

在进化的内共生过程中，宿主细胞内的细胞器的构型和结构因共生体的引入而被扰乱，然后这些细胞器与共生体的细胞器一起在宿主细胞的遗传和生理调节下重新排列为新的细胞。特别是，在高等分类单元中发现的亚细胞多样性可以被认为是由每个细胞中的这些肿瘤和细胞器的重排引起的，这是由不难假设这些现象通常在系统发育上不同的分类群中产生。使用属于紫菜目、红藻门的单细胞红藻，在其细胞器（例如叶绿体-核染色）的焦点上检查了它们的亚细胞多样性。通过在共焦激光显微镜和荧光显微镜下通过荧光染料，我们可以区分出一系列构型其细胞器结构呈阶梯式或阶梯式形态，如下；1）光合细胞器体积增大，表面扩大，细胞核不在细胞中心；2）嵌入的蛋白核变为裸露的核，叶绿体呈现出趋势从细胞的中心部分到外围，3)蛋白核和细胞核表现出从细胞外围到细胞中心部分的趋势， 4）它们有时会相互竞争占据细胞的中心部分，但细胞核最终占据细胞的中心部分，5）蛋白核被推离细胞中心部分消失，叶绿体位于细胞外围，其数量发生变化这些结果支持了 Hara 和 Chihara (1992) 发表的基于其超微结构、栖息地、无性繁殖类型和植物合成色素组成的系统学。相比之下，他们不能支持 Hara、Yokoyama 和 Kim (2000) 基于其 18SrDNA 和 psbA 检查的分子系统发育关系。