Cytological studies of the relationship between endocytosymbiosis and arrangement and configuration of organelles in algal cells with special emphasis on the evolutionary significance.

细胞学研究藻类细胞内细胞共生与细胞器排列和构型之间的关系，特别强调进化意义。

基本信息

批准号：
10440245
负责人：
HARA Yoshiaki
金额：
$ 4.35万
依托单位：
Faculty of Science, Yamagata University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B).
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2000
项目状态：
已结题

项目摘要

On the process of evolutionary endosymbiosis, configuration and structure of organelles in the host cell have been turburated by the invation of symbionts. After that, these organelles together with symbiont's ones were rearranged for a novel cell under the genetical and physiological regulation of the host cell. In particular, subcellular diversity found in higher taxa can be considered to be caused by these tuburance and rearrangement of organelles in each cell which was established by endocytosymbiosis. It is not so difficult to assume that these phenomina commonly give rise among phylogenetically different taxa.Using unicellular red algae belonging to the Porphyridiales, Rhodophyta, their subcellular diversity were examined on the focus of their organelles such as chloroplast-pyrenoid and nucleus stained by fluoresent dye under conforcal laser microscope and fluorescent microscopes.As a result, we can distinguished a seies of configurational clain or stepwise morphologies on their … More organellar sturucutres as followings ; 1) photosynthetic organelles increasing their volum and expanding thier surface and a nucleus located not in the center of a cell, 2) embedded pyrenoids changing into naked ones, and chloroplast body showing tendency from central part of cell to the periphery, 3) pyrenoid and nucleus showing the tendency frm cell periphery to the central part of cell, 4) they sometimes compete to occupy the central part of cell with each other, however, nucleus finally occupy the central part of cell, 5) the pyrenoid pushed away from the central part of cell disappearing and chloroplast located cell periphery changing its number in a cell from single to multiple. These results supprted the systematics based on their ultrastructure, habitat, types of the asexual reproduction and composition of phytosynthetic pigments published by Hara and Chihara (1992). In contrast, they could not support the molecular phylogenetic relationship examined by Hara, Yokoyama and Kim (2000) based on their 18SrDNA and psbA Less

在进化性内共生的过程中，宿主细胞中细胞器的构型和结构已受符号的邀请。之后，在宿主细胞的遗传和物理调节下，将这些细胞器与象征主义的细胞器一起重新排列。特别是，在较高分类单元中发现的亚细胞多样性可以被认为是由内养生无关的每个细胞中细胞内的细胞和重排引起的。并不难以假设这些现象在系统发育不同的群体中通常会产生。使用孢子虫的单细胞红色静脉，属于叶绿素和细胞核的细胞细胞的焦点，并在叶绿素中染上了荧光大分子，并在其细胞器的重点上检查其亚细胞多样性可以在其…更有机的结构上区分出构型认证或逐步形态的案例，如以下内容； 1）光合作用细胞器增加其体积并扩大了地表和不在电池中心的核us，2）嵌入的荷叶类拟南芥变成肉肉，以及叶绿体的身体从细胞的中部部分到外周的趋势，3）pyrenoid and firm pyrip frm perip perip perip perip perip perip perip perip perip perip perip perip the中心，是4）中央部分，4）4）然而，核心最终占据了细胞的中央部分，5）类拟南类动物从细胞消失的中央部分推出，叶绿体位于细胞周围的细胞周围将其数量从单个变为多个。这些结果根据Hara和Chihara（1992）出版的植物合成色素的超微结构，栖息地，无性繁殖和植物合成色素的组成的类型抑制了系统。相比之下，他们无法支持Hara，Yokoyama和Kim（2000）基于18srDNA和PSBA检查的分子系统发育关系。