In-situ Remediation of Contaminated Soil with Chlorinated Organic Compounds using Natural Activity

利用自然活性对含氯有机化合物污染的土壤进行原位修复

• 批准号: 17206089
• 负责人: INOUE Chihiro
• 金额: $ 32.12万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17206089/
• 关键词: Soil Remediation; Chlorinated Organic Compounds; Dechlorination Reaction; Pyrite; Iron Sulfide; Microorganisms; MNA; Dioxin; 有機塩素系化合物

The purpose of this study was to develop remediation system for contaminated soils with chlorinated organic compounds using effectively with dechlorination reactions with natural minerals and microorganisms. Main research results are as follows.1. Dechlorination reaction of TCE with pyrite progressed well in aerobic condition rather than in anaerobic condition. TCE was converted to carbon dioxide and chlorine ion via organic acids. Pyrite was oxidized and converted to sulfate and iron ion. Reaction rate of this reaction depended on the oxygen concentration.2. TCE dechlorination with sulfide minerals such as iron sulfide, chalcopyrite and so on progressed in anaerobic condition. Pyrite gave maximum reaction rate in the experiment.3. TCE was completely dechlorinated to ethane by anaerobic microbial consortium obtained from sulfate reducing environment. It was found that control of methane producing bacteria was important factor for the TCE decomposition by this consortium.4. Long-term data for the attenuation of chlorinated organic compounds in groundwater in several contaminated sites were collected and analyzed. Main factor of the attenuation was revealed by the analysis.5. Behavior of chlorinated organic compounds such as TCE in the contaminated site was predicted using transport model containing adsorption to soil particles, diffusion in groundwater and decomposition with microbial and mineralogical reaction of the compounds.6. From these results, soil remediation process contaminated with chlorinated organic compounds was basically designed. The combined process consists of mixing of pyrite with the contaminated soil and activation of degrading bacteria in the microbial community.7. Chlorinated aromatic compounds such as chlorobenzenes were also decomposed by pyrite in aerobic and anaerobic systems. These results indicate the possibility of decomposition of dioxins and their derivatives by pyrite in aqueous system.

本研究的目的是开发有效利用天然矿物质和微生物脱氯反应的含氯有机化合物污染土壤的修复系统。主要研究成果如下： 1． TCE与黄铁矿的脱氯反​​应在有氧条件下比在厌氧条件下进展顺利。三氯乙烯通过有机酸转化为二氧化碳和氯离子。黄铁矿被氧化并转化为硫酸盐和铁离子。该反应的反应速率取决于氧浓度。 2．硫化铁、黄铜矿等硫化矿物的TCE脱氯是在厌氧条件下进行的。黄铁矿在实验中给出了最大的反应速率。 3．三氯乙烯被硫酸盐还原环境中获得的厌氧微生物群落完全脱氯为乙烷。结果表明，对产甲烷菌的控制是该联合体分解TCE的重要因素。 4．收集并分析了几个污染地点地下水中氯化有机化合物衰减的长期数据。分析揭示了影响衰减的主要因素。 5．利用包含对土壤颗粒的吸附、在地下水中的扩散以及化合物的微生物和矿物反应分解的传输模型，预测了污染场地中三氯乙烯等氯化有机化合物的行为。 6．根据这些结果，基本设计了受氯化有机化合物污染的土壤修复工艺。该组合过程包括黄铁矿与污染土壤的混合以及微生物群落中降解细菌的激活。7．氯代芳香族化合物如氯苯也在需氧和厌氧系统中被黄铁矿分解。这些结果表明二恶英及其衍生物在水体系中可能被黄铁矿分解。

项目成果

Long-Term Persistence of Cyclodiene Pesticide in Soil

环二烯农药在土壤中的长期残留

• 发表时间: 2007
• 期刊: 4th International Workshop on Water Dynamics, AIP Conference Proceedings 898
• 作者: 原田浩平;他;J. Hara and Y. Kawabe
• 通讯作者: J. Hara and Y. Kawabe

長期間継代培養した嫌気微生物群によるTCEの無害化

厌氧微生物长期传代对TCE的解毒作用

• 发表时间: 2006
• 期刊: 平成18年度日本生物工学会大会講演要旨集
• 作者: 伊勢孝太郎;須藤孝一;井上千弘
• 通讯作者: 井上千弘

Long-term Anaerobic Subculture Obtained from a TCE-contaminated Site

从 TCE 污染场地获得的长期厌氧亚培养物

• 发表时间: 2006
• 期刊: Proceedings of the Society for Biotechnology, Japan Annual Meeting 2006
• 作者: Qi;ZHANG;Jyun;HATAKEYAMA;Lu;ZHOU;Yu;GUO;Makoto;SAKURAI;Hideo;KAMEYAIVIA;S. Sato et. al.;金森敏幸;T. Morikawa et. al.;Keigo Matsuda;M. Kitsunezuka et. al.;K. Ise et. al.
• 通讯作者: K. Ise et. al.

Dechlorination of Chlorinated Organic Compounds with Sulfide Minerals

用硫化矿物脱氯有机氯化物

• 发表时间: 2005
• 期刊: Proceedings of 11th Symposium on Groundwater and Soil Contamination and Remediation
• 作者: T.;Kanamori;J. Hara et. al.
• 通讯作者: J. Hara et. al.

Evaluation of Clostridium Isolated from Sulfate Reducing Condition

硫酸盐还原条件下分离梭菌的评价

• 发表时间: 2007
• 期刊: Proceedings of MMIJ Annual Meeting 2007
• 作者: A.;Okamura;T.;Hagiwara;S.;Yamagami;M.;Yamaguchi;T.;Kanamori;T.;Shinbo;S.;Kondo;K.;Miwa;I.;Itagaki;K.Sumaru;Y. Takahashi et. al.
• 通讯作者: Y. Takahashi et. al.