Development of Green Chemistry-oriented Analytical Method for Environmental and Waste Samples by Nano/Micro LC/MS

开发面向绿色化学的环境和废物样品纳/微LC/MS分析方法

• 批准号: 17201020
• 负责人: SUZUKI Shigeru
• 金额: $ 19.55万
• 依托单位: Chubu University (2006-2007)National Institute for Environmental Studies (2005)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17201020/
• 关键词: Nano; Micro LC; MS; SOLUTE FOCUSING TECHNIQUE; IONIZATION METHOD BY EXCITED ARGON; HIGHLY HYDROPHOBIC COMPOUNDS; Environmental and Waste Samples; 環境化学物質; グリーンケミストリ分析; 検出器開発; 試料導入方法; 疎水性成分

Micro liquid chromatography/mass spectrometry (LC/MS) can contribute green chemistry by saving solvent and reducing waste. However micro LC/MS was mostly ineffective for measuring highly hydrophobic compounds, in particular for small molecules. We developed a micro LC/MS method for measuring hydrophobic and small molecular mass compounds of environmental interest. The method includes solute focusing technique in micro LC and an ionization method by excited argon for mass spectrometry.Solute focusing was successfully performed for highly hydrophobic compounds by dynamically diluting hydrophobic solution with hydrophilic solution in micro mixer upstream of analytical micro column. In case of O-(4-cyano-2-ethoxybenzyl) hydroxylamine (CNET) derivatives of aldehyde homologues in acetone, theoretical plate numbers of the peaks were ranged 27, 000to 60, 000 when 5uL of the solution in 0.2to 10ppb were injected. Working curves of those compounds were mostly linear with good regression.Ionization by excited argon was efficient for some hydrophobic compounds. It generated M+'ion with high signal to noise ratio (s/n) from butanone, of which the ionization energy (I.E.) and the proton affinity (PA) are 9.52eV and 827.3kJ/mol, respectively. M+'ion and MH+ ion generated from picoline (I.E.: 9.26eV, PA: 949.1kJ/mol) were respectively 8 and 30 times higher in s/n than those from Atmospheric Pressure Chemical Ionization(APCI).Through this study, a way to save and reduce organic solvents, which contribute green chemistry in environmental and waste analysis, was developed

微量液相色谱/质谱 (LC/MS) 可以通过节省溶剂和减少浪费来贡献绿色化学。然而，微型 LC/MS 对于测量高度疏水性化合物（尤其是小分子）大多无效。我们开发了一种微量 LC/MS 方法，用于测量环境相关的疏水性和小分子质量化合物。该方法包括微型LC中的溶质聚焦技术和用于质谱分析的激发氩电离方法。通过在分析微柱上游的微混合器中用亲水性溶液动态稀释疏水性溶液，成功地对高度疏水性化合物进行了溶质聚焦。对于丙酮中醛同系物的 O-(4-氰基-2-乙氧基苄基) 羟胺 (CNET) 衍生物，当注入 5uL 0.2 至 10ppb 溶液时，峰的理论板数范围为 27, 000 至 60, 000 。这些化合物的工作曲线大多是线性的，具有良好的回归性。激发氩电离对于一些疏水性化合物是有效的。它从丁酮中产生高信噪比（s/n）的M+'离子，其电离能（I.E.）和质子亲和力（PA）分别为9.52eV和827.3kJ/mol。甲基吡啶产生的 M+' 离子和 MH+ 离子（即：9.26eV，PA：949.1kJ/mol）的信噪比分别是大气压化学电离（APCI）产生的 8 倍和 30 倍。通过这项研究，一种方法开发了节省和减少有机溶剂的方法，这有助于环境和废物分析中的绿色化学

项目成果

Mass Spectrometry in Environmental Analysis

环境分析中的质谱分析

• 发表时间: 2007
• 期刊: J. Mass Spectrom. Soc. Jpa. 55
• 作者: N.Naruse;Y.Mera;K.Maeda;鈴木 茂;S.Liang;Shigeru Suzuki
• 通讯作者: Shigeru Suzuki

SOLUTE FOCUSING TECHNIQUE AND IONIZATION METHOD BY EXCITED ARGON FOR ANALYZING HIGHLY HYDROPHOBIC COMPOUNDS OF ENVIRONMENTAL INTEREST BY MICRO MICRO LIQUID CHROMATOGRAPHY/MASS SPECTROMETRY

激发氩气溶质聚焦技术和微量液相色谱/质谱分析高疏水环保化合物的电离方法

• 发表时间: 2008
• 作者: N.Naruse;Y.Mera;K.Maeda;鈴木 茂;S.Liang;Shigeru Suzuki;Y Iguchi;A.Yajima;鈴木 茂;Y.Nakamura;S.Suzuki
• 通讯作者: S.Suzuki

DEVELOPMENT OF A HIGHLY-SENSITIVE MICRO-LC/MS/MS METHOD FOR THE ELUCIDATION OF TOXICOKINETICS OF DIPHENYL ARSENIC ACID IN CENTRAL NERVOUS SYSTEM

开发高灵敏度微量 LC/MS/MS 方法来阐明二苯砷酸在中枢神经系统中的毒代动力学

• 发表时间: 2007
• 作者: 山田憲太;佐藤弘紹;駒口哲也;目良裕;前田康二;S. Suzuki;M.Yoshikane
• 通讯作者: M.Yoshikane

SOLUTE FOCUSING TECHNIQUE AND IONIZATION METHOD BY EXCITED ARGON FOR ANALYZING HIGHLY HYDROPHOBIC COMPOUNDS OF ENVIRONMENTAL INTEREST BY MICRO LIQUIDCHROMATOGRAPHY/MASS SPECTROMETRY

微量液相色谱/质谱分析高疏水性环保化合物的激发氩溶质聚焦技术和电离方法

• 发表时间: 2008
• 作者: S. Suzuki;A. Hasegawa;M. Uebori;Y. Yoshida;S. Shibata;M. Yoshikane
• 通讯作者: M. Yoshikane

SOLUTE FOCUSING TECHNIQUE AND IONIZATION METHOD BY EXCITED ARGON FOR ANALYZING HIGHLY HYDROPHOBIC COMPO UNDS OF ENVIRONMENTAL INTEREST BY MICRO LIQUID CHRO MATOGRAPHY/MASS SPECTROMETRY

微量液相色谱/质谱分析高疏水环保化合物的激发氩溶质聚焦技术和电离方法

• 发表时间: 2008
• 作者: 山田憲太;佐藤弘紹;駒口哲也;目良裕;前田康二;S. Suzuki
• 通讯作者: S. Suzuki