Ecologycal characterization of polyphosphate accumulating organisms in enhanced biological phosphorus removal Processes and its application

强化生物除磷过程中聚磷积累生物的生态学特征及其应用

基本信息

批准号：
16201016
负责人：
MINO Takashi
金额：
$ 25.63万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2006
项目状态：
已结题

项目摘要

Following five investigations were conducted to clarify the ecophisiology of polyphosphate accumulating organisms (PAOs). (1) Population structure of PAOs in full scale wastewater treatment plants (UTPs), (2) screening for novel groups of PAOs, (3) development of MAR-FISH method for the quantitative evaluation of anaerobic carbon uptake by PAOs and glycogen accumlating organisms (GAOs), (4) the effect of pH on the anaerobic carbon uptake by PAOs and GAOs, and (5) microbial interactions within activated sludge.In (1), DAPI staining for polyphosphate granules was applied to the specimens analyzed with FISH. It was found that Accumulibacter phosphatis and Actino1011 positive Actinobacteria accounted for almost or more than half of total PAOs. In (2), we developed an oligonucleotide probe that detect novel PAOs in Actinobacteria. Also, we confirmed the existence of PAOs in Gammaproteobacteria. From (3) and (4), it was found that pH is not a key factor that affect anaerobic carbon uptake performances of PAOs and GAOs. Yet, our result is contradictory to the outcomes reported by other research groups. In (5), we showed that unknown factors in activated sludge or its supernatant affect the performances of PAOs. Further, we isolated bacteriophages that are lytic to Microlunatus phosphovorus, one of the few isolated PAOs.In conclusion, we confirmed the existence of different PAOs. And the present study reveiled basic ecophysiology of PAOs. Yet, in order to achieve better and more stable performance of enhanced biological phosphorus removal, it is needed to more closely understand the relationships between different microoganisms.

进行了五次研究以阐明聚磷酸盐积累生物（PAO）的生态学。（1）PAO在全尺度废水处理厂（UTP）的人口结构，（2）筛选新的PAO组，（3）在PAOS和糖原累积的生物体（GAOS）累积PAOS和糖基因糖的厌氧碳吸收厌氧碳吸收（GAOS）的定量评估（GAOS），（4）PA对PA的影响（4）Paos的影响（4））的影响（4）污泥（1），将多磷酸盐颗粒的DAPI染色应用于用鱼类分析的标本。发现累积的磷酸磷酸杆菌和Atacino1011阳性阳性阳性阳性占总PAO的一半以上。在（2）中，我们开发了一种寡核苷酸探针，该探针检测出静脉细菌中的新PAO。另外，我们证实了γ-细菌中PAO的存在。从（3）和（4）中发现，pH不是影响PAOS和GAOS厌氧碳摄取性能的关键因素。但是，我们的结果与其他研究小组报道的结果相矛盾。在（5）中，我们表明活化污泥或其上清液中未知因素会影响PAO的性能。此外，我们分离了裂解的噬菌体，这些噬菌体是磷磷酸的，这是少数孤立的PAO之一。总而言之，我们证实了存在不同的PAO。本研究重新验证了PAO的基本生态生理学。然而，为了在增强的生物磷去除的更好，更稳定的性能中，需要更紧密地了解不同的微型人民之间的关系。