Ecologycal characterization of polyphosphate accumulating organisms in enhanced biological phosphorus removal Processes and its application

强化生物除磷过程中聚磷积累生物的生态学特征及其应用

基本信息

批准号：
16201016
负责人：
MINO Takashi
金额：
$ 25.63万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2006
项目状态：
已结题

项目摘要

Following five investigations were conducted to clarify the ecophisiology of polyphosphate accumulating organisms (PAOs). (1) Population structure of PAOs in full scale wastewater treatment plants (UTPs), (2) screening for novel groups of PAOs, (3) development of MAR-FISH method for the quantitative evaluation of anaerobic carbon uptake by PAOs and glycogen accumlating organisms (GAOs), (4) the effect of pH on the anaerobic carbon uptake by PAOs and GAOs, and (5) microbial interactions within activated sludge.In (1), DAPI staining for polyphosphate granules was applied to the specimens analyzed with FISH. It was found that Accumulibacter phosphatis and Actino1011 positive Actinobacteria accounted for almost or more than half of total PAOs. In (2), we developed an oligonucleotide probe that detect novel PAOs in Actinobacteria. Also, we confirmed the existence of PAOs in Gammaproteobacteria. From (3) and (4), it was found that pH is not a key factor that affect anaerobic carbon uptake performances of PAOs and GAOs. Yet, our result is contradictory to the outcomes reported by other research groups. In (5), we showed that unknown factors in activated sludge or its supernatant affect the performances of PAOs. Further, we isolated bacteriophages that are lytic to Microlunatus phosphovorus, one of the few isolated PAOs.In conclusion, we confirmed the existence of different PAOs. And the present study reveiled basic ecophysiology of PAOs. Yet, in order to achieve better and more stable performance of enhanced biological phosphorus removal, it is needed to more closely understand the relationships between different microoganisms.

进行了以下五项研究来阐明聚磷酸盐积累生物（PAO）的生态生理学。 (1) 大型污水处理厂 (UTP) 中 PAO 的种群结构，(2) 筛选新的 PAO 类群，(3) 开发 MAR-FISH 方法，用于定量评估 PAO 和糖原累积生物体的厌氧碳吸收(GAO)，(4) pH 对 PAO 和 GAO 厌氧碳吸收的影响，以及 (5) 活性污泥内微生物的相互作用。 (1)、对用FISH分析的标本进行聚磷酸盐颗粒的DAPI染色。结果发现，Accumulibacter phosphatis和Actino1011阳性Actinobacteria几乎占PAO总数的一半以上。在 (2) 中，我们开发了一种寡核苷酸探针，可检测放线菌中的新型 PAO。此外，我们还证实了 Gammaproteobacteria 中存在 PAO。由(3)和(4)可知，pH并不是影响PAOs和GAOs厌氧吸碳性能的关键因素。然而，我们的结果与其他研究小组报告的结果相矛盾。在（5）中，我们表明活性污泥或其上清液中的未知因素会影响PAO的性能。此外，我们还分离出了能够裂解 Microlunatus phosphorus 的噬菌体，这是少数分离的 PAO 之一。总之，我们证实了不同 PAO 的存在。本研究揭示了 PAO 的基本生态生理学。然而，为了实现更好、更稳定的强化生物除磷性能，需要更深入地了解不同微生物之间的关系。