Assessmemt of embryo viability and its microenvironment
胚胎活力及其微环境的评估
基本信息
- 批准号:06671632
- 负责人:
- 金额:$ 1.41万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for General Scientific Research (C)
- 财政年份:1994
- 资助国家:日本
- 起止时间:1994 至 1995
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Microtechiniques and highly sensitive methods such as enzymatic cycling, micro-Western analysis, reverse transcription polymerase chain reaction and so on were employed to study these processes. Low hexokinase activity and high activities of enzymes in the phosphate pathway were characteristic of immature oocytes. During maturation, the activities of hexokinase and phosphofructokinase increased significantly. These changes were used to analyze involvement of epidermal growth factor (EGF) and prostaglandins (PG) in oocyte maturation. EGF is shown to stimulate maturation by increasing PG production in granulosa cells. Electro-physiologically, the sensitivity of oocyte to inositol triphosphate increased and Ca^<2+> release system developed during maturation. Progesterone production of oocyte and embryos are shown by enzymatic cycling and other methods using radiometry. This hormone produced by embryos themselves may play a role in embryonic development in intracrine fashion. There is 100-fold increase in glucose uptake from oocyte to blastocyst in mice. A switch in substrate preference of the embryo from pyruvate to glucose during preimplantation development may be explained by increases in the activity of hexokinase and expression of glucose transporter, GLUT1. Hexokinase activities determined by NADP cycling increased 20-fold while expression of GLUT1 assessed by micro-Western method 10-fold. GLUT1 expression was also analyzed by RT-PCR,which indicated that the expression is regulated at transcription level. There is a delay in the developmental changes in glucose uptake, hexokinase activity and GLUT1 expression when the embryos are developed in vitro. It is of interest to note that EGF stimulates these developmental changes in vitro. In conclusion, expanding knowledge in the process of oocyte maturation and embryonic development may serve not only as significant clinical implications for in vitro fertilization techniques but as breakthroughs in gynecology.
采用了微技术和高度敏感的方法,例如酶循环,微西分析,逆转录聚合酶链反应等来研究这些过程。低己糖激酶活性和磷酸盐途径中酶的高活性是未成熟卵母细胞的特征。在成熟过程中,己糖酶和磷酸果糖酶的活性显着增加。这些变化用于分析表皮生长因子(EGF)和前列腺素(PG)参与卵母细胞成熟。 EGF被证明通过增加颗粒细胞中的PG产生来刺激成熟。在电学上,卵母细胞对三磷酸肌醇的敏感性增加,Ca^<2+>释放系统在成熟过程中发展。使用辐射测定法显示了卵母细胞和胚胎的孕激素的产生和其他方法。胚胎本身产生的这种激素可能会以内在的方式在胚胎发育中发挥作用。从卵母细胞到胚泡的小鼠葡萄糖吸收增加了100倍。在植入前发育过程中,胚胎从丙酮酸到葡萄糖的底物偏好转换可以通过己糖激酶的活性和葡萄糖转运蛋白的表达glut1来解释。通过NADP循环确定的己糖激酶活性增加了20倍,而GLUT1的表达通过微西方法评估10倍。 RT-PCR还分析了GLUT1表达,该表达表明该表达在转录水平下受到调节。当胚胎在体外发育时,葡萄糖摄取,己糖激酶活性和GLUT1表达的发育变化延迟。值得注意的是,EGF在体外刺激了这些发育变化。总之,在卵母细胞成熟和胚胎发育过程中扩大知识不仅可以作为体外受精技术的重要临床意义,而且可以像妇科中的突破一样。
项目成果
期刊论文数量(74)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
堤 治他 望月、武谷編: "図説産婦人科" メジカルビュー社, (1994)
Osamu Tsutsumi 等人,Takeya 编辑:《妇产科图解》Medical View Publishing,(1994 年)
- DOI:
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- 影响因子:0
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- 通讯作者:
Y.Kasai, O.Tsutsumi, T.Taketani and M.Iino: "Stretch-induced enhancement of contractions in uterine smooth muscles of rats." Journal of Physiology. 468. 373-384 (1995)
Y.Kasai、O.Tsutsumi、T.Taketani 和 M.Iino:“拉伸诱导的大鼠子宫平滑肌收缩增强”。
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- 影响因子:0
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- 通讯作者:
N. Hakuno, et al.: "Fas/APO-1/CD95 system as a mediator of granulosa cell apoptosis in ovarian follicle atresia." Endocrinology. (in press).
N. Hakuno 等人:“Fas/APO-1/CD95 系统作为卵巢卵泡闭锁中颗粒细胞凋亡的介质。”
- DOI:
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- 影响因子:0
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- 通讯作者:
Y. Morita O. Tsutsumi, I. Oka, H. Katagiri and Y. Taketani:"Application of reverse tramscription-polymerase chain reaction to study glucose transporter protein GLUT1 expression in preimplantation on mouse embryos" Journal of Mammalian ova Research. 11. 1-
Y. Morita O. Tsutsumi、I. Oka、H. Katagiri 和 Y. Taketani:“应用逆转录聚合酶链反应研究小鼠胚胎植入前葡萄糖转运蛋白 GLUT1 的表达”《哺乳动物卵子研究杂志》。
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- 影响因子:0
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- 通讯作者:
O. Tsutsumi, T. Iida, N. Nakahori, Y. Taketani: "Hormone Research" Analys is of the testis-determining gene SRY in Patients with XY gonadat dy sgenesis (in press),
O. Tsutsumi、T. Iida、N. Nakahori、Y. Taketani:“激素研究”分析是 XY 性腺发育不全患者的睾丸决定基因 SRY(正在印刷中),
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- 影响因子:0
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TSUTSUMI Osamu其他文献
TSUTSUMI Osamu的其他文献
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{{ truncateString('TSUTSUMI Osamu', 18)}}的其他基金
White-color luminescence from a single material with harmony between order- and disorder-structures of luminogen aggregates
单一材料的白色发光,发光体聚集体的有序结构和无序结构之间的协调
- 批准号:
15K05613 - 财政年份:2015
- 资助金额:
$ 1.41万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Self-organization of gold nanoparticles and photochemical manipulation of their organized structures by means of liquid-crystalline molecules
金纳米粒子的自组织及其通过液晶分子对其组织结构进行光化学操纵
- 批准号:
21710094 - 财政年份:2009
- 资助金额:
$ 1.41万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
Development of Photoresponsive Organic-Inorganic Hybrid Liquid Crystals : Spontaneous Organization of Inorganic Nanoparticles with Organic Molecules
光响应有机-无机杂化液晶的开发:无机纳米粒子与有机分子的自发组织
- 批准号:
19559003 - 财政年份:2007
- 资助金额:
$ 1.41万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
A laboratory and clinical study of the mechanism of mother-to-child infection of Toxoplasma
弓形虫母婴感染机制的实验室及临床研究
- 批准号:
15591732 - 财政年份:2003
- 资助金额:
$ 1.41万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
STUDIES ON FACTORS RELATED TO EMBRYO DEVELOPMENT TO IMPROVE OUTCOME OF ASSITED REPRODUCTIVE TECHNOLOGY
研究胚胎发育相关因素以提高辅助生殖技术的效果
- 批准号:
12671580 - 财政年份:2000
- 资助金额:
$ 1.41万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
BASIC STUDIES FOR IMPROVEMENT OF IN VITRO FERTILIZATION AND CRYOPRESERVATION
改进体外受精和冷冻保存的基础研究
- 批准号:
10671518 - 财政年份:1998
- 资助金额:
$ 1.41万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Analysis of the microenvironment and cryopreservation of embryos on their development
胚胎发育的微环境和冷冻保存分析
- 批准号:
08671870 - 财政年份:1996
- 资助金额:
$ 1.41万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Biochemical and endocrinological analysis of oocytes and embryos
卵母细胞和胚胎的生化和内分泌分析
- 批准号:
03670771 - 财政年份:1991
- 资助金额:
$ 1.41万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
Physiological roles of growth factors in mammalian reproduction.
生长因子在哺乳动物繁殖中的生理作用。
- 批准号:
63570771 - 财政年份:1988
- 资助金额:
$ 1.41万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
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