Effect of serum from patients with restenosis after PTCA on migration-stimulating activity of cultured smooth muscle cells.

PTCA 术后再狭窄患者血清对培养的平滑肌细胞迁移刺激活性的影响。

基本信息

批准号：
06670739
负责人：
HAMADA Masanori
金额：
$ 1.34万
依托单位：
Wakayama Medical College (W.M.C.)
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06670739/
关键词：
PTCA re-stenosis cultured cell migration

项目摘要

Background Migration of medial smooth muscle cells into the intima of the artery, with subsequent intimal cell proliferation, is one of main features of the development of restenosis after percutaneous transluminal coronary angioplasty (PTCA). Although several growth and/or migration-stimulating factors have been studied as possible candidates for a role in the development of restenosis after PTCA,the relative importance of each of these factors is unknown. The aim of this study was to measure migration-stimulating and mitogenic activities of human serum on standard cultured smooth muscle cells and to see if there was any relationship between thses activities and the incidence of restenosis after PTCA.Methods and Results The migration-stimulating activity of serum from patients who underwent PTCA on smooth muscle cells was assayd by the multi-well chemotaxis chamber method as described by Tsukamoto. The mitogenic activity was assayaed by colorimetric method by neutral red as described by Borenfreund. The migration-stimulating activity, but not the mitogenic activity, was significantly increased in the restenotic group along with elevation of coronary score, serum LP (a), triglyceride and phosphate ion concentration. A higher incidence of restenosis after PTCA was detected in patients with diabetes mellitus (DM) as compared to those without DM.Conclusion Human serum, like fetal calf serum, contains a factor (s) that stimulates migration of cultured aortic smooth muscle cells through a membran filter. This migration-stimulating activity was greatly enhanced in the serum of patients who developed restenosis after PTCA.

内侧平滑肌细胞向动脉内膜迁移，随后发生内膜细胞增殖，是经皮易流质冠状动脉血管成形术（PTCA）后再狭窄的主要特征之一。尽管已经研究了几种生长和/或刺激因素作为候选者在PTCA后再狭窄中发挥作用，但这些因素的相对重要性尚不清楚。这项研究的目的是测量人血清对标准培养的平滑肌细胞的迁移刺激和有丝分裂活性，并查看PTCA后THSES活动与再狭窄的发生率之间是否存在任何关系，并导致迁移刺激活性。如Tsukamoto所述，通过多孔趋化室法测定了在平滑肌细胞上接受PTCA的患者的血清。如Borenfreund所述，通过中性红色法对有丝分裂活性进行了测定。迁移活性，但没有促丝分裂活性，再生造成群体显着增加，以及冠状动脉评分，血清LP（A），甘油三酸酯和磷酸离子离子浓度的升高。与没有DM的糖尿病患者（DM），检测到PTCA后的再狭窄发生率更高，与没有DM的灌注者相比，人血清（如胎儿小腿血清）含有一个因子（S）刺激培养的主动脉平滑肌通过A的迁移的因素膜过滤器。在PTCA后发生再狭窄的患者的血清中，这种迁移刺激的活性大大增强。