The study of interstitial thermochemotherapy using Implant Heating System for oral cancer

种植体加热系统间质热化疗治疗口腔癌的研究

基本信息

批准号：
05807191
负责人：
TOHNAI Iwai
金额：
$ 1.28万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1995
项目状态：
已结题

项目摘要

Thermotolerance and local immune response in oral mucoas were observed for basic research of thermochem otherapy using Implant Heating System(IHS). Hsp40 was induced not only by heating but also sodium arsenite and prostaglandin in the same as hsp70. The extent of thermotolerance correlated well with the relative amount of hsp70 and 40. These results suggested that hsp40 is good predictor of thermotolerance in addition to hsp70. To examine effects of heat on immunologically responsive cells in oral mucosa, mouse tongue were heated by IHS.The densities of Langerhans cells and Thy-1-positive cells rapidly increased within 3 h after the treatment. Electron microscopy confirmed that Langerhans cells in the tongue epithelium formed clusters with lymphcytic cells, suggesting an active response to the hyperthermia. In clinical trial, 19 patients with oral cancer were treated by thermochemotherapy using IHS.Thirteen patients of 19 underwent thermochemotherapy before operation. As a results, CR was observed in 11 patients and PR in 2 patients, and postoperative pathological examination showed no residual tumor cells in 12 patients. Other 6 patients were only umderwent thermochemotherapy without operation. As a results, CR was observed in all 6 patients. It was suggested that thermochemotherapy using IHS is promising as a new therapy for oral cancer, because it dose not necessitate surgery or radiotherapy and hence improves the QOL of patient with oral cancer.

利用种植体加热系统（IHS）观察口腔粘膜的耐热性和局部免疫反应，用于热化疗的基础研究。 Hsp40不仅可以通过加热诱导，而且与hsp70一样也可以通过亚砷酸钠和前列腺素诱导。耐热性的程度与 hsp70 和 40 的相对量密切相关。这些结果表明，除了 hsp70 之外，hsp40 也是耐热性的良好预测因子。为了检查热对口腔粘膜免疫反应细胞的影响，用 IHS 加热小鼠舌头。治疗后 3 小时内，朗格汉斯细胞和 Thy-1 阳性细胞的密度迅速增加。电子显微镜证实舌上皮中的朗格汉斯细胞与淋巴细胞形成簇，表明对热疗有积极的反应。在临床试验中，19例口腔癌患者采用IHS进行热化疗。19例患者中，13例在术前接受了热化疗。结果，11例患者获得CR，2例患者获得PR，12例患者术后病理检查未见残留肿瘤细胞。其余6例仅接受热化疗，未进行手术。结果，6名患者全部达到CR。有人认为，使用 IHS 的热化疗有望成为口腔癌的新疗法，因为它不需要手术或放疗，因此可以改善口腔癌患者的生活质量。