Study on the interleukin-2 producing capacity in the patients with periodontitis

牙周炎患者白细胞介素2产生能力的研究

• 批准号: 04671159
• 负责人: ARAI Hideo
• 金额: $ 1.41万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04671159/
• 关键词: Periodontitis; Interleukin-2; Intracellular signal transduction; T cell; CD3; Interleukin-2(IL-2); IL-2レセプター; 若年性歯周炎; 細胞内Ca^<2+>濃度; phorbol myristate acetate(PMA); ionomycine; 細胞内情報伝達系

The purpose of this study was to assess the role of interleukin-2 (IL-2) in the pathogenesis of periodontitis. The levels of IL-2 produced from mononuclear cells were measured after stimulating with a CD3 monoclonal antibody (CD3 mAb) in 45 patients (12 with juvenile periodontitis [JP], 20 with rapidly progressive periodontitis and 13 with adult periodontitis) and 20 healthy subjects (HS). Six subjects including 3 JP patients demonstrated elevated IL-2 level. When phorbol myristate (PMA) was substituted for monocytes, there existed one JP patient with significantly high IL-2 producing capacity, and two JP patients and one HS with low capacity. To investigate the cause of their unusual IL-2 producing capacities, intracellular signal transduction system in T cells was examined. The levels of IL-2 produced upon stimulation with ionomycin and PMA were proportionate to those produced upon stimulation with CD3 mAb and PMA in the subjects examined except one JP patient ; suggesting that the elevated IL-2 producing capacity of this patient is related to the intracellular calcium ion level after stimulation of CD3 molecules. These results suggest that IL-2 producing capacity is responsible for the pathogenesis of a certain type of early-onset periodontitis, and that intracellular signal transduction system is concerned with the imbalance of IL-2 production in some individuals.

本研究的目的是评估白介素 2 (IL-2) 在牙周炎发病机制中的作用。在 45 名患者（12 名青少年牙周炎 [JP]、20 名快速进展性牙周炎和 13 名成人牙周炎）和 20 名健康受试者中用 CD3 单克隆抗体 (CD3 mAb) 刺激后，测量了单核细胞产生的 IL-2 水平。 （HS）。包括 3 名 JP 患者在内的 6 名受试者表现出 IL-2 水平升高。当佛波醇肉豆蔻酸酯(PMA)替代单核细胞时，存在1名JP患者具有显着高的IL-2产生能力，以及2名JP患者和1名HS具有低能力。为了研究其异常产生 IL-2 能力的原因，对 T 细胞的细胞内信号转导系统进行了检查。除一名 JP 患者外，在受检查的受试者中，用离子霉素和 PMA 刺激后产生的 IL-2 水平与用 CD3 mAb 和 PMA 刺激后产生的 IL-2 水平成正比；提示该患者IL-2产生能力升高与CD3分子刺激后细胞内钙离子水平有关。这些结果表明，IL-2的产生能力与某些类型的早发性牙周炎的发病有关，并且细胞内信号转导系统与某些个体中IL-2产生的失衡有关。