Study on the interleukin-2 producing capacity in the patients with periodontitis

牙周炎患者白细胞介素2产生能力的研究

• 批准号: 04671159
• 负责人: ARAI Hideo
• 金额: $ 1.41万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04671159/
• 关键词: Periodontitis; Interleukin-2; Intracellular signal transduction; T cell; CD3; Interleukin-2(IL-2); IL-2レセプター; 若年性歯周炎; 細胞内Ca^<2+>濃度; phorbol myristate acetate(PMA); ionomycine; 細胞内情報伝達系

The purpose of this study was to assess the role of interleukin-2 (IL-2) in the pathogenesis of periodontitis. The levels of IL-2 produced from mononuclear cells were measured after stimulating with a CD3 monoclonal antibody (CD3 mAb) in 45 patients (12 with juvenile periodontitis [JP], 20 with rapidly progressive periodontitis and 13 with adult periodontitis) and 20 healthy subjects (HS). Six subjects including 3 JP patients demonstrated elevated IL-2 level. When phorbol myristate (PMA) was substituted for monocytes, there existed one JP patient with significantly high IL-2 producing capacity, and two JP patients and one HS with low capacity. To investigate the cause of their unusual IL-2 producing capacities, intracellular signal transduction system in T cells was examined. The levels of IL-2 produced upon stimulation with ionomycin and PMA were proportionate to those produced upon stimulation with CD3 mAb and PMA in the subjects examined except one JP patient ; suggesting that the elevated IL-2 producing capacity of this patient is related to the intracellular calcium ion level after stimulation of CD3 molecules. These results suggest that IL-2 producing capacity is responsible for the pathogenesis of a certain type of early-onset periodontitis, and that intracellular signal transduction system is concerned with the imbalance of IL-2 production in some individuals.

这项研究的目的是评估白介素-2（IL-2）在牙周炎发病机理中的作用。用CD3单克隆抗体（CD3 MAB）刺激45例患者（12例患有少年牙周炎[JP]，20例患有迅速进行性牙周炎的幼体牙周炎和13例患有成人牙周炎）和20个健康受试者（HS）（HS），用CD3单克隆抗体（CD3 MAB）刺激后测量了从单核细胞产生的IL-2水平。包括3名JP患者在内的六名受试者显示IL-2水平升高。当将佛波尔肉豆蔻酸酯（PMA）取代单核细胞时，就有一名IL-2产生能力高的JP患者，两名JP患者和一名HS患者和一个HS容量较低。为了研究其异常IL-2产生能力的原因，研究了T细胞中细胞内信号转导系统。用离子霉素和PMA刺激后产生的IL-2水平与在检查受试者中用CD3 mAb和PMA刺激后产生的IL-2水平与一名JP患者刺激的水平成正比；表明该患者的IL-2产生能力升高与刺激CD3分子后的细胞内钙离子水平有关。这些结果表明，IL-2产生能力负责某种类型的早期牙周炎的发病机理，并且细胞内信号转导系统与某些个体的IL-2产生不平衡有关。