Role and therapy of renal medullary dysfunction as a risk factor for chronic renal failure

肾髓质功能障碍作为慢性肾功能衰竭危险因素的作用和治疗

• 批准号: 04670383
• 负责人: NOGUCHI Hiroshi
• 金额: $ 1.34万
• 依托单位: Tokyo Medical & Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04670383/
• 关键词: Chronic renal failure; Renal Medullary Dysfunction; ADH; Endothelin-1; ANP; hyperosmolality; IMCD; glomerulus; 心房性ナトリウム利尿ペプチド; 抗利尿ホルモン; 髄質内層準合尿細管; 浸透圧

Kidney plays a main role for body fluid homeostasis. Urine concentration is one of most important renal function as well as glomerular filtration rate(GFR). Inner medullary collecting ducts(IMCD) is quite important for urine concentration. We reported that the site of renal production of endothelin-1(ET-1) is glomerulus and IMCD.Hyperosmolality stimulated ET-1 production in IMCD, whereas it inhibited ANP-dependent cGMP accumulation in IMCD.WE measured plasma and urinary concentration of ADH, ET-1, and ANP in control subjects and patients with chronic renal failure(CRF). Urinary excretion of ADH in patients with CRF was reduced, while plasma levels of ADH was not different from healthy subjects. In contrast, plasma level of ET-1 was elevated in patients with CRF with no difference in plasam level from control subjects. There was no difference in plasma ANP level between control subjects and CRF patients.These data suggest that the decrease of urinary excretion of ADH and the increase of plasma ET-1 level may have some role decreased renal function in patients with chronic renal failure.

肾脏对体液稳态起主要作用。尿液浓度是最重要的肾功能之一，以及肾小球过滤率（GFR）。内部髓质收集管（IMCD）对于尿液浓度非常重要。我们报道说，内皮素-1（ET-1）的肾脏产生部位是肾小球和IMCD。hyperosmolity刺激了IMCD中的ET-1产生，而它抑制了IMCD中的ANP依赖性CGMP。 ，ET-1和ANP在对照受试者和慢性肾衰竭（CRF）的患者中。 CRF患者ADH的尿液排泄降低，而血浆ADH水平与健康受试者没有差异。相比之下，CRF患者的ET-1的血浆水平升高，与对照受试者的Plasam水平无差异。对照受试者和CRF患者之间的血浆ANP水平没有差异。这些数据表明，ADH的尿排泄减少和血浆ET-1水平的增加可能在慢性肾衰竭患者中肾功能降低。

项目成果

Kimio Tomita: "Effects of ET-1 on woter and chloride transport in contical collecting ducts of the rat" Am.J.Physiol.264. (1993)

Kimio Tomita：“ET-1 对大鼠圆锥集合管中沃特和氯离子转运的影响”Am.J.Physiol.264。

Y.Terada, K.Tomita, H.Nonoguchi, T.Yang, F.Marumo: "Expression of endothelin-3 mRNA along rat nephron segments using polymerase chain reaction." Kidney Int.44. 1273-1280 (1993)

Y.Terada、K.Tomita、H.Nonoguchi、T.Yang、F.Marumo：“使用聚合酶链式反应沿大鼠肾单位片段表达内皮素 3 mRNA。”

Kimio Tomita: "Effects of EFI on water and chloide transport in cortical collecting duets of the rat" Am.J.Physiol.264. F690-F696 (1993)

Kimio Tomita：“EFI 对大鼠皮质集合二重奏中水和氯离子运输的影响”Am.J.Physiol.264。

Y.Terada, K.Tomita, H.Nonoguchi, F.Marumo: "Polymerase chain reaction localization of constitutive nitric oxide synthase and soluble guanylate cyclase messenger RNAs in microdissected rat nephron segments." J.Clin.Invest.909. 659-665 (1992)

Y.Terada、K.Tomita、H.Nonoguchi、F.Marumo：“显微解剖的大鼠肾单位片段中组成型一氧化氮合酶和可溶性鸟苷酸环化酶信使 RNA 的聚合酶链反应定位。”

H.Nonoguchi, K.Abe, F.Komatsu: "Recombinant human erythropoietin for autologous blood donation." Lancet. 339. 1483 (1992)

H.Nonoguchi、K.Abe、F.Komatsu：“用于自体献血的重组人类促红细胞生成素。”