Intracelluar signal transduction of relaxin : comparative study with beta-adrenergic action

松弛素的细胞内信号转导：与β-肾上腺素能作用的比较研究

• 批准号: 04670049
• 负责人: OSA Takuro
• 金额: $ 1.28万
• 依托单位: Yamaguchi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04670049/
• 关键词: Mg; Mn; relaxin; isoprenaline; db cAMP; 8-Br cGMP; myometrium; mag-fura2; forskolin; protein kinase A; myometrium; Mg; Mn; relaxin; isoprenaline; db cAMP; 8-Br cGMP; myometrium; mag-fura2; forskolin; protein kinase A; myometrium

It was aimed how the relaxin action resembles and differs from cAMP-and cGMP-mediated effects. The longitudinal myometrium was taken from ovariectomized rat 72h after the injection of estradiol-benzoate. Phasic contractions were evoked in Mg-free Krebs solution by electrical stimulation. The [Mg]i was increased from 380muM in the control solution to 420muM, when the tissue was exposed to Ca-free 40mX K solution containing 15mM Mg. It was assumed that muscle cells were loaded with Mn, when exposed to 0.6mM Mn. Contractile responses to relaxin, isoprenaline, forskolin and cyclic nucleotide derivatives were compared before and after the intracellular load with Mg or Mn. Results are summarized in the table shown below.Influence of the elevation of intracellular Mg(b) of Mu(c) on the contractile inhibition caused by db cAMP and 8-bromo cGMP in comparison with that by isoprenaline, forskolin and relaxin. Upward and downward arrows indicate augmentation and attenuation of the inhibitory effects, respectively.The activation of protein kinase A is thought to be a common pathway of relaxin and adrenergic beta-action (Hsu and Sanborn, 1986). The questions are raised, how and why relaxin and db cAMP behabed differently from isoprenaline and forskolin under the influence of intracellular load with Mn.

这是针对松弛素的作用与营地和CGMP介导的效果的相似之处。注射雌二醇 - 苯甲酸酯后，从卵巢切除的大鼠72H取走纵向子宫肌术。通过电刺激，在无MG的Krebs溶液中引起了阶段收缩。当组织暴露于含有15mm mg的无CA的40mx K溶液时，[mg] I从对照溶液中的380MUM增加到420MUM。假设在暴露于0.6mm Mn时，将肌肉细胞加载了MN。收缩对松弛素，异丙肾上腺素，福斯科蛋白和环状核苷酸衍生物的反应在用Mg或Mn的细胞内载荷之前和之后进行了比较。结果总结在下面所示的表中。与异丙肾上腺素，输水蛋白和松弛素相比，由DB CAMP和8-BROMO CGMP引起的收缩抑制作用和8-Bromo CGMP引起的收缩抑制作用上的细胞内Mg（B）升高。向上和向下的箭头分别表示抑制作用的增强和衰减。蛋白激酶A的激活被认为是放松蛋白和肾上腺素能β-效果的常见途径（Hsu and Sanborn，1986）。提出了这些问题，如何以及为什么放松蛋白和DB营地与异丙肾上腺素和福斯科林在细胞内负载与MN的影响下不同。