Host-Environment Relationship of essential hypertension by using genetic marker and DNA polymorphisms

利用遗传标记和DNA多态性研究原发性高血压的宿主-环境关系

• 批准号: 62480170
• 负责人: TANAKA Heizo
• 金额: $ 3.84万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-62480170/
• 关键词: SHRSP; serum lipids; catecholamine; callicrein; sulfur-containing amino acids; apo protein B-100; Southern blotting method; DNA mutation; 遺伝・環境解析; 本態性高血圧; 脳卒中易発症性高血圧自然発症ラット; アポリポ蛋白Β-100; アポリポ蛋白Bー100

We examined age-related changes in serum lipids, callicrein, catecholamine, and amino acids using the stroke prone spontaneously hypertensive rats (SHRSP) and the contrasting normotensive Wister-Kyoto rats (WKY). Both strains were raised for 6 months from 5 weeks af ter birth to 6 months. Difference of changes was observed in total cholesterol (Ch), VLDL-, LDL-, HDL- Ch and phospholipids in serum from 5 weeks after birth between SHRSP and WKY. The genetic abnormality of lipid metabolism in SHRSP was confirmed. Further, increases of catecholamine and callicrein in urine and long stay of Na in body were recognized.Those results suggest that sympathetic system and body fluid may be concerned in high blood pressure. The amount of urinary excretion of sulfur-containing amino acids like taurine was higher in SHRSP than in WKY.Genetic analysis of Apo protein B-100 concerned in cholesterol, especially LDL-CH was made by Southern blotting methods. SHRSP and WKY liver DNAs were digested with 14 kinds of restriction enzymes. The used probes were 7 fragment of human Apo protein B full length cDNA. Significant differences of RFLP between SHRSP and WKY DNAs could not be found. It is concluded that there is no mutation on the cutting sites of restriction enzymes we used, and big delation of genetic constitution. But, it cannot be denied the possibility that we may find mutations using other restriction enzymes.

我们使用易发生中风的自发性高血压大鼠 (SHRSP) 和对比血压正常的 Wister-Kyoto 大鼠 (WKY) 检查了血清脂质、红利素、儿茶酚胺和氨基酸的年龄相关变化。两种菌株均饲养6个月，从出生后5周到6个月。 SHRSP和WKY之间观察到生后5周血清中总胆固醇(Ch)、VLDL-、LDL-、HDL-Ch和磷脂的变化差异。证实了SHRSP脂质代谢的遗传异常。此外，还发现尿中儿茶酚胺和卡利醛的增加以及Na在体内的长时间滞留。这些结果表明交感系统和体液可能与高血压有关。 SHRSP组尿中牛磺酸等含硫氨基酸的排泄量高于WKY组。采用Southern印迹法对与胆固醇、特别是LDL-CH相关的Apo蛋白B-100进行基因分析。 SHRSP 和 WKY 肝脏 DNA 用 14 种限制性酶消化。所用探针为人Apo蛋白B全长cDNA的7片段。未发现 SHRSP 和 WKY DNA 之间 RFLP 的显着差异。结论是我们所使用的限制性内切酶的酶切位点没有发生突变，遗传结构也没有发生很大的变化。但是，不能否认我们可能使用其他限制性酶发现突变的可能性。