Host-Environment Relationship of essential hypertension by using genetic marker and DNA polymorphisms

利用遗传标记和DNA多态性研究原发性高血压的宿主-环境关系

• 批准号: 62480170
• 负责人: TANAKA Heizo
• 金额: $ 3.84万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-62480170/
• 关键词: SHRSP; serum lipids; catecholamine; callicrein; sulfur-containing amino acids; apo protein B-100; Southern blotting method; DNA mutation; 遺伝・環境解析; 本態性高血圧; 脳卒中易発症性高血圧自然発症ラット; アポリポ蛋白Β-100; アポリポ蛋白Bー100

We examined age-related changes in serum lipids, callicrein, catecholamine, and amino acids using the stroke prone spontaneously hypertensive rats (SHRSP) and the contrasting normotensive Wister-Kyoto rats (WKY). Both strains were raised for 6 months from 5 weeks af ter birth to 6 months. Difference of changes was observed in total cholesterol (Ch), VLDL-, LDL-, HDL- Ch and phospholipids in serum from 5 weeks after birth between SHRSP and WKY. The genetic abnormality of lipid metabolism in SHRSP was confirmed. Further, increases of catecholamine and callicrein in urine and long stay of Na in body were recognized.Those results suggest that sympathetic system and body fluid may be concerned in high blood pressure. The amount of urinary excretion of sulfur-containing amino acids like taurine was higher in SHRSP than in WKY.Genetic analysis of Apo protein B-100 concerned in cholesterol, especially LDL-CH was made by Southern blotting methods. SHRSP and WKY liver DNAs were digested with 14 kinds of restriction enzymes. The used probes were 7 fragment of human Apo protein B full length cDNA. Significant differences of RFLP between SHRSP and WKY DNAs could not be found. It is concluded that there is no mutation on the cutting sites of restriction enzymes we used, and big delation of genetic constitution. But, it cannot be denied the possibility that we may find mutations using other restriction enzymes.

我们使用易于自发的高血压大鼠（SHRSP）和对比鲜明的wister-kyoto大鼠（WKY）检查了血清脂质，Callicrein，Catecholamine和氨基酸与年龄相关的变化。两种菌株从出生后5周升高到6个月。从SHRSP和WKY之间的5周后，在血清中血清中的总胆固醇（CH），VLDL-，LDL-，HDL-CH和磷脂的总胆固醇（CH）（CH）（CH）（CH）中观察到了差异。 SHRSP中脂质代谢的遗传异常已得到证实。此外，尿液中儿茶酚胺和callicrein在体内的长期停留中增加了。 SHRSP中含硫氨基酸的尿液排泄量高于WKY。胆固醇中有关的Apo蛋白B-100的遗传分析，尤其是LDL-CH，尤其是通过Southern印迹方法制造的。用14种限制酶消化SHRSP和WKY肝DNA。使用的探针是7个人apo蛋白B全长cDNA的片段。找不到SHRSP和WKY DNA之间RFLP的显着差异。可以得出结论，我们使用的限制酶的切割部位没有突变，也没有遗传构成的大范围。但是，不能否认我们可能会使用其他限制酶发现突变的可能性。