Studies on Transmission of Hemorrhagic Fever with Renal Syndrome (HFRS) Virus in Rodents

肾综合征出血热（HFRS）病毒在啮齿动物中传播的研究

基本信息

批准号：
62480087
负责人：
HASHIMOTO Nobuo
金额：
$ 3.84万
依托单位：
Hokkaido University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1989
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-62480087/
关键词：
Hemorrhagic fever with renal syndrome (HFRS)HFRS virus Hantavirus Rodent Transmission of HFRS virus Epizootiology of HFRS Ecology of HFRS virus Viral Zoonoses げっ歯類媒介性人畜共通伝染病ドブネズミウィルス性ズノーシスげつ菌類媒介性人畜共通伝染病 HFRSウイルスげつ歯類 HFRSウイ

项目摘要

The mode of HFRS virus transmission among rodents was examined by comparing the results of epidemiological surveys of urban rats in endemic areas and experimental infection of laboratory rats. The results are as follows.1) The urban rats in the endemic focus have shown the age-dependent acquisition of infection. HFRS virus was mostly isolated from lung tissue in adult rats showing high antibody titers.2) A total four sero-positive small field mice, two Clethrionomys rufocanus bedfordiae, one Apodemus argenteus hokkaidi and one Apodemus speciosus ainu, were detected around the endemic focus indicating an evidence of transmission of HFRS virus from the infected urban rats to indigenous field mice.3) Two strains of HFRS virus from Rattus, SR-11 and KI-262, showed identical antigenicity but deferred in virulence against newborn rats. SR-11 strain isolated from a laboratory rat developed clinical signs and died at about 18 days after inoculation. KI-262 strain from an urban rat in an endemi … More c focus, did not show any clinical signs. Necrosis of neurons in the brain tissue occurred in the rats infected with SR-11, while it was mild in the animals infected with KI-262.4) When rats-were inoculated with SR-11, sero-conversion rate was almost similar in newborn and adult rats regardless of inoculation routes.5) IgG antibody was transferred from immune dams to their offspring prenatally as well as postnatally. IgG antibody was detected in the fetus sera. In newborn rats, maternal IgG antibody reached a peak titer at two weeks after birth, then declined and disappeared at about 8 weeks of age. The protective effect of maternal antibody remained in 10 week-old rats.6) Antibody dependent enhancement (ADE) of the infection of HFRS virus was studied using macrophage-like cell lines. ADE of the infection occurred with monoclonal antibodies (MAbs) to both Gl and G2 envelope glycoproteins, but not with MAbs to nucleocapsid protein. Antigenic determinants related to hemagglutination and virus neutralization were found to cause ADE of the infection Less

通过比较流行地区城市大鼠的流行病学调查结果和实验室大鼠的实验感染结果，探讨了HFRS病毒在啮齿类动物中的传播方式。结果如下： 1）流行病点城市大鼠表现出年龄分布。 HFRS 病毒主要从成年大鼠的肺组织中分离出来，显示出高抗体滴度。2) 共有 4 只血清阳性小田鼠，两只 Clethrionomys rufocanus bedfordiae，一只Apododemus。在流行病灶周围发现了北海银线鼠和阿伊努线姬鼠，这表明有证据表明 HFRS 病毒从受感染的城市老鼠传播到本地田鼠。3) 来自黄鼠的两种 HFRS 病毒株，SR-11 和 KI-262， SR-11 菌株表现出相同的抗原性，但对新生大鼠的毒力有所延迟，该菌株出现临床症状，并在接种后约 18 天死亡。来自城市鼠的 KI-262 毒株感染了 SR-11 后，没有表现出任何临床症状，而感染 KI 的动物则出现轻微的脑组织坏死。 -262.4) 当大鼠接种 SR-11 时，无论接种途径如何，新生大鼠和成年大鼠的血清转化率几乎相似。5) IgG 抗体从免疫母鼠转移到其后代胎儿血清中检测到IgG抗体，出生后2周母体IgG抗体滴度达到峰值，8周龄左右下降并消失，母体抗体的保护作用仍然存在。 6) 使用巨噬细胞样细胞系研究了 HFRS 病毒感染的抗体依赖性增强 (ADE)，这两种细胞的单克隆抗体 (MAb) 均发生感染的 ADE。 G1和G2包膜糖蛋白，但不与核衣壳蛋白的单克隆抗体一起发现，与血凝和病毒中和相关的抗原决定簇导致感染的ADE较少。