The breeding for development of myocardial infarction in WHHL rabbits

WHHL兔心肌梗死的培育

• 批准号: 61480436
• 负责人: WATANABE Yoshio
• 金额: $ 3.65万
• 依托单位: Kobe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1988
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-61480436/
• 关键词: WHHL rabbit; selected breeding; Coronary atherosclerosis; 選抜交配; WHHL rabbit; 冠状動脈の狭窄病変; 心筋梗塞; 冠状動脈硬化; 心筋梗塞好発モデル; コレステロール; 冠状動脈粥状硬化

The Watanabe heritable hyperlipidemic (WHHL) rabbit shows hyperlipidemia from birth, owing to a genetical deficiency of the low density lipoprotein receptors. The aortic and coronary atheros-clerosis occur at a premature age in this animal. However, myocardial infarction does not develop often in the yound rabbits. We carried out selective breeding in an attempt to increase in the incidence of this lision in WHHL rabbits. In this study 88 rabbits after selective breeding (MI-line) sere compored with 93 rabbits before selective breeding (non MI-line) in order to estimate the breeding effect. The results were as follows : 1). The average of the percent of narrowing was 56% in non MI-line, 66% in F1 of MI-line, 77% in F3 of MI-line and 78% in F4 of MI-line. 2). The frequency of over 75% narrowing was 26% in non MI-line and increased to 41% in F1, 67% in F3 and 75% in F4 of MI-line. 3). The incidence in myocardial infarction was 13% in non MI-line and increased to 15% in F1, 26% in F3 and 25% in F4 of MI-line. 4). WHHL rabbits aged over 10 months in which myocardial infarction provoked were always observed over 75% narrowing in coronary arteries, whereas in the rabbits aged under 10 months there were no relation between myocardial infarction and the severe coronary narrowing. 5). The incidence of myocardial infarction in the male rabbits was significantly higher than that in the female. Consequently, we considered that both coronary narrowing and myocardial infarction were expanded because of the selective breeding.

由于低密度脂蛋白受体的遗传缺乏，渡边可遗传的高脂血症（WHHL）兔从出生开始表现出高脂血症。主动脉和冠状动脉 - 终止发生在该动物的过早年龄。但是，心肌梗塞在幼犬中并不经常发育。我们进行了选择性育种，以试图增加WHHL兔子的发病率。在这项研究中，选择性育种后的88只兔子（Mi-Line）在选择性育种（非MI-Line）之前，在选择性繁殖（非Mi-line）之前，以估算繁殖效果。结果如下：1）。非MI线的缩小百分比为56％，在MI线的F1中为66％，MI-Line的F3为77％，MI-Line的F4中为78％。 2）。超过75％的狭窄频率在非MI线中为26％，F1的频率为41％，在F3中为67％，MI-Line的F4频率为75％。 3）。心肌梗塞的发病率在非MI线中为13％，F1的发病率为15％，在MI线的F4中为26％，在F3中增加了25％。 4）。在10个月内，在冠状动脉动脉中始终观察到超过75％的心肌梗死的兔子，而在10个月以下的兔子中，心肌梗塞和严重的冠状动脉狭窄之间没有关系。 5）。雄性兔子中心肌梗塞的发生率明显高于女性。因此，我们认为，由于选择性育种，冠状动脉变窄和心肌梗塞都扩大了。

项目成果

M.,Shiomi;T.,Ito;Y.,Watanabe: Lab.Anim.Sci.37(1). 84-88 (1987)

M.，潮见；T.，伊藤；Y.，渡边：Lab.Anim.Sci.37(1)。

Kaoru Hatanaka: American Heart Journal. 113(1). (1987)

Kaoru Hatanaka：美国心脏杂志。

K.,Hatanaka;T.,Ito;M.,Shiomi;A.,Yamamoto;Y.,Watanabe: Ameri.Heart J.113(2). 280-288 (1987)

K.，Hatanaka；T.，Ito；M.，Shiomi；A.，Yamamoto；Y.，渡边：Ameri.Heart J.113(2)。

Masashi Shiomi: Biochimica et Biophysica Acta. 917(1). 92-100 (1987)

Masashi Shiomi：生物化学与生物物理学学报。

K.Hatanaka; T.Ito; M.Shiomi; A.Yamamoto; Y.Watanabe: "Ischemic heart disease in the WHHL rabbits : A model for myocardial injury in genetically hyperlipidemic animal." Ameri. Heart J.113(2). 280-288 (1987)

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