NEUROENDOCRINOLOGICAL ROLES OF HISTAMINE IN THE HYPOTHALAMO-HYPOPHYSEAL SYSTEM

组胺在下丘脑-垂体系统中的神经内分泌作用

基本信息

批准号：
60480135
负责人：
YAMATODANI Atsushi
金额：
$ 3.97万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1985
资助国家：
日本
起止时间：
1985 至 1986
项目状态：
已结题

项目摘要

From the distribution of the histaminergic neuron system in rat brain, elucidated in our laboratory, it is considered that histamine plays some important roles as a neuromodulator in the hypothalamo-hypophyseal neuroendocrine system. To know its roles in the neuroendocrine regulation, we studied changes in histamine concentration in the hypothalamus after physiologically stimulating hypophyseal hormone secretion and effects of depletion of neuronal histamine using alfa-fluoromethylhistidine (FMH), a specific inhibitor of histidine decarboxylase, on the hormone level in plasma. The results are as follows.1. In the posterior hypothalamic area which contains cell bodies of the histaminergic neurons, we found a clear circadian rhythm in histamine contents. After chronic i.c.v. administration of FMH, the clear diurnal variation of the plasma corticosterone levels was diminished.2. Bilateral adrenalectomy caused decrease of histamine contents in the posterior hypothalamus and increase of those in the anterior hypothalamus. After chronic i.p. administration of FMH, the marked increase of plasma ACTH by bilateral adrenalectomy was completely suppressed.3. Administration of hyperosmotic saline caused increase of both plasma vasopressin levels and hypothalamic histamine contents. Chronic administration of FMH i.c.v. suppressed the increase of plasma vasopressin induced by the saline injection.In addition, using in vitro sequential perifusion system, we found involvement of <H_1> -receptor in LH-RH secretion. Furthermore, we examined the detailed distribution of histaminergic terminals in the rat brain using a more sensitive method for detecting histaminergic terminals, and found that dense networks of the fibers in the hypothalamic nuclei, such as the paraventricular and supraoptic nucleus, the median eminence, and neurohypophysis.These neurochemical and histochemical findings support our hypothesis, that is, histamine is a neuromodulator in the neuroendocrine system.

从本实验室阐明的大鼠脑组织胺能神经元系统的分布来看，组胺作为神经调节剂在下丘脑-垂体神经内分泌系统中发挥着重要作用。为了了解其在神经内分泌调节中的作用，我们研究了生理刺激垂体激素分泌后下丘脑组胺浓度的变化以及使用组氨酸脱羧酶的特异性抑制剂阿法氟甲基组胺（FMH）消耗神经元组胺对激素水平的影响在血浆中。结果如下： 1．在包含组胺能神经元细胞体的下丘脑后部区域，我们发现组胺含量具有明显的昼夜节律。慢性静脉注射后FMH给药后，血浆皮质酮水平的明显昼夜变化减弱。2．双侧肾上腺切除导致下丘脑后部组胺含量减少，下丘脑前部组胺含量增加。慢性腹腔注射后FMH给药后，双侧肾上腺切除引起的血浆ACTH显着升高被完全抑制。 3．施用高渗盐水导致血浆加压素水平和下丘脑组胺含量增加。 FMH 静脉注射长期给药此外，利用体外序贯灌注系统，我们发现<H_1>受体参与LH-RH的分泌。此外，我们使用更灵敏的组胺能末端检测方法检查了大鼠大脑中组胺能末端的详细分布，发现下丘脑核团中的密集纤维网络，例如室旁核和视上核、正中隆起和这些神经化学和组织化学发现支持我们的假设，即组胺是神经内分泌系统中的神经调节剂。