A ROLE OF CYTOKINE IN PROGRESSION OF HEARTFAILRE

细胞因子在心力衰竭进展中的作用

基本信息

批准号：
05670592
负责人：
YAMAGUCHI Seiji
金额：
$ 1.34万
依托单位：
Yamagata University School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05670592/
关键词：
CONGESTIVE HEART FAILURE TUMOR NECROSIS FACTOR (TNF)SOLUBLE TNF RECEPTORS CYTOKINE TNFα soluble TNF receptor heart failure cachexia myocardium

项目摘要

Previous investigations have suggested that circulating cytokines (i.e., TNFa) are increased in patients with advanced heart failure (HF). We investigated whether regulatory peptides, two types of naturally occurring soluble TNF receptors (sTNF-RI and sTNF-RII) are increased in patients with HF.These peptides can prevent the adverse pathologic sequela caused by TNF.We assayd plasma from 37 patients with HF (NYHA II 16, III 14, IV 7) and the age-matched control subjects(n=24) with Enzyme-Linked Immunoassay (ELISA). TNFa had no significant difference between heart failure patients and normal subjects (7.7<plus-minus> 1.7 (mean<plus-minus> SEM) vs6.9 <plus-minus> 1.2pg/ml ; ns). However, soluble TNF-RI was significantly higher in patients with heart failure than normal subjects (1629 <plus-minus> 153vs681 <plus-minus> 53pg/ml ; p<0.01) and soluble TNF-RII was also higher in patients with heart failure than control subjects (2124 <plus-minus> 157vs3005 <plus-minus> 196pg/ml ; p<0.01). Further, soluble TNF-RI was increased in relation to the severity of heart failure (normal subjects ; 681 <plus-minus> 53pg/ml, NYHA class II ; 1226 <plus-minus> 115pg/ml, NYHA class III ; 1624 <plus-minus> 207pg/ml, NYHA class IV ; 2789 <plus-minus> 568pg/ml). Similarly, there was an increase in soluble TNF-RII with symptomatic severity in heart failure (normal subjects ; 2124 <plus-minus> 157pg/ml, NYHA class II ; 2555 <plus-minus> 140pg/ml, NYHA class III ; 2909 <plus-minus> 262pg/ml, NYHA class IV ; 4500 <plus-minus> 764pg/ml). The levels of soluble TNF R-1 were related to those of soluble TNF R-II in heThese results suggest that 1) in a variety of tissues in HF patients, the released soluble receptors may inhibit TNF bioactivity by binding the molecule and preventing ligand to the cellular TNF receptors and 2) the levels of soluble TNF receptors may reflect the severity of congestive heart failure.

先前的研究表明，晚期心力衰竭 (HF) 患者的循环细胞因子（即 TNFa）增加。我们研究了调节肽（两种天然存在的可溶性 TNF 受体（sTNF-RI 和 sTNF-RII））在心力衰竭患者中是否增加。这些肽可以预防 TNF 引起的不良病理后遗症。我们对 37 名心力衰竭患者的血浆进行了检测(NYHA II 16、III 14、IV 7) 和年龄匹配的对照受试者 (n=24) 采用酶联免疫分析 (ELISA)。心力衰竭患者和正常受试者之间的 TNFa 没有显着差异（7.7±1.7（平均±SEM）vs6.9±1.2pg/ml；ns）。然而，心力衰竭患者的可溶性 TNF-RI 显着高于正常受试者（1629 <正负> 153vs681 <正负> 53pg/ml；p<0.01），心力衰竭患者的可溶性 TNF-RII 也较高比对照受试者失败（2124<正负>157vs3005<正负>196pg/ml； p<0.01）。此外，可溶性 TNF-RI 的增加与心力衰竭的严重程度相关（正常受试者；681 <正负> 53pg/ml，NYHA II 级；1226 <正负>115pg/ml，NYHA III 级；1624 <正负> 207pg/ml，NYHA IV 级；2789 <正负> 568皮克/毫升）。同样，随着心力衰竭症状的严重程度，可溶性 TNF-RII 也有所增加（正常受试者；2124 <正负> 157pg/ml，NYHA II 级；2555 <正负>140pg/ml，NYHA III 级；2909 <正负值> 262pg/ml，NYHA IV 级 4500 <正负值> 764皮克/毫升）。体内可溶性 TNF R-1 水平与可溶性 TNF R-II 水平相关。这些结果表明：1）在心力衰竭患者的多种组织中，释放的可溶性受体可能通过结合分子并阻止配体结合来抑制 TNF 生物活性。细胞 TNF 受体和 2) 可溶性 TNF 受体水平可能反映充血性心力衰竭的严重程度。