Experimentary study of pathology and treatment of spinal cord injury.

脊髓损伤病理及治疗的实验研究。

• 批准号: 03454365
• 负责人: MURASE Masaaki
• 金额: $ 1.09万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-03454365/
• 关键词: Spinal cord injury; Thoromboxane B_2; 6-keto-prostaglandin F_<1alpha>; Leukotriene C_4; Vascular damage; blood sugar; Fos-protein; Spinal regencration; Fos蛋白; 6ケトプロスタグランジンF_<12>; MRI; αトコフェロ-ル; ビタミンE

1)Thromboxane A_2(TXA_2)is known to be an eicosanoid with potent platelet aggregation and vasoconstricting activity, while prostaglandin I_2(PGI_2)antagonizes its activity. These stable degradation products, TXB_2 and 6-keto-PGF_1alpha, were inbalanced in rats spinal cord compression injury model. The former reached a peak 5 minutes after compression injury, while the later slight increased. When methyl predonisolone sodium succinate(MPSS) was administered intravenously after compression injury, the increased production of TXB_2 was inhibited. While the 6-keto-PGF_1alpha level was not affected in spite of administration of MPSS. We also planed to study the effect of other drugs such as 21-aminosteroid in several duration and dose of administration. Leukotoriene C_4(LTC_4)production increased peak values 10 minutes after compression in a guinea pigspinal cord. The increased production of LTC_4 was inhibits by about 50%, when flavonoid was administered 15 minutes prior to compression injury. Also the administration of Indomethasine 30 minutes before injury inhibited by about 90% of the increased production of LTC_4. These results suggest that the LCT_4 production in the injured spinal cords is related to secondly disorder after spinal cord injury, spinal edema, particularly.2)In experiment of the generation after spianl cord injury, we tried to identify the c-Fos protein, which are some products from the proto-oncogenes c-fos, and to research the condition of nerve activity on generation of spinal cord immunohistologically. The condition of stain was examined in endcrine cells in hypothlamus of rats. In this study, we proved to get good stain condition when we use the ABC staining in the vibratome section that is fixed with 4% paraformmaldehyde. We will study if it is possible to use this method in frozened section of spinal cord, and to apply ABC staining in the experiment model of the generation of spinal cord.

1)血栓烷A_2(TXA_2)是一种类二十烷酸，具有有效的血小板聚集和血管收缩活性，而前列腺素I_2(PGI_2)则拮抗其活性。这些稳定的降解产物TXB_2和6-酮-PGF_1α在大鼠脊髓压迫损伤模型中不平衡。前者在压缩损伤后5分钟达到峰值，而后者略有增加。压缩性损伤后静脉注射甲基泼尼松龙琥珀酸钠(MPSS)时,TXB_2的产生增加受到抑制。尽管施用 MPSS，6-酮-PGF_1α 水平并未受到影响。我们还计划研究其他药物（例如 21-氨基类固醇）在不同给药时间和剂量下的效果。豚鼠脊髓受压 10 分钟后，白三烯 C_4(LTC_4) 的产生峰值增加。当在压缩性损伤前 15 分钟施用类黄酮时，LTC_4 的产生增加被抑制约 50%。此外，在损伤前30分钟施用吲哚美辛抑制了约90%的LTC_4增加的产生。这些结果表明，损伤脊髓中LCT_4的产生与脊髓损伤后的第二种疾病，特别是脊髓水肿有关。 2)在脊髓损伤后的生成实验中，我们试图鉴定c-Fos蛋白，该蛋白是脊髓损伤后产生的一种蛋白。是原癌基因c-fos的一些产物，并通过免疫组织学研究神经活动对脊髓生成的状况。检查大鼠下丘脑内分泌细胞的染色情况。在这项研究中，我们证明当我们在用 4% 多聚甲醛固定的振动切片上使用 ABC 染色时，可以获得良好的染色条件。我们将研究是否可以将该方法应用于脊髓冰冻切片，并将ABC染色应用于脊髓生成的实验模型中。

项目成果

李 暁光,井形 高明,村瀬 正昭,山田 秀大,吉栖 悠輔: "急性脊髄損傷モデルにおける血糖値の影響" 日本整形外科学会雑誌. 65. 1028 (1991)

李晓光、井形贵明、村濑正明、山田秀弘、吉须佑介：“急性脊髓损伤模型中血糖水平的影响”日本骨科学会杂志 65. 1028 (1991)。

三橋 雅,井形 高明,藤内 武春,森本 訓明: "損傷脊髄におけるロイコトリエンC_4の変動" 日本整形外科学会雑誌. 65. 33-39 (1991)

Masaru Mitsuhashi、Takaaki Igata、Takeharu Fujiuchi、Noriaki Morimoto：“受伤脊髓中白三烯 C_4 的变化”日本骨科学会杂志 65. 33-39 (1991)。

福澤 健治: "抗酸化剤" 治療学. 26. 109-117 (1992)

Kenji Fukuzawa：“抗氧化剂”治疗学。26. 109-117 (1992)。

福井 義浩: "Development of neurons and synapses in Ochratoxin A-induced mirocephalic mice:Aquantitative assessment of somatosensory cortex" Neurotoxicology & Teratology. 14. 191-196 (1992)

Yoshihiro Fukui：“赭曲霉毒素 A 诱导的小头小鼠中神经元和突触的发育：体感皮层的定量评估”《神经毒理学与畸胎学》14. 191-196 (1992)。

TAKASHI FUKUSHIMA,et.al.: "Magnetic Resonance Imaging Study on Spinal Cord Plasticity in Patients with Cervical Compression Myelopathy" SPINE. 16. 534-538 (1991)

TAKASHI FUKUSHIMA 等人：“颈椎压迫性脊髓病患者脊髓可塑性的磁共振成像研究”SPINE。