A study on T cell differentiation, selection and T cell receptors

T细胞分化、选择和T细胞受体的研究

基本信息

批准号：
03454192
负责人：
ONOE Kazunori
金额：
$ 3.97万
依托单位：
Hokkaido University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1993
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-03454192/
关键词：
Thymus T cell differentiation Positive selection Negative selection CD4^-8^-Programd cell death NK1^+TCRalphabeta cell Fas antigen positive selection negative selection programmed cell death NK1.1^+TCRαβ^+胸腺細胞 CD4^+8^-T細胞骨髄キメラ

项目摘要

1. Expression of Mtv-7, the genetic locus of an endogenous mammary tumor virus which determines the minor lymphocyte stimulatory (Mls--1^<a\0> phenotype, results in the deletion of the cells that express Vbeta 6, 7, 8.1, or 9 among developing thymocytes. By using allogeneic [Mls-1^b -> Mls-1^a] bone marrow chimeras, we have demonstrated that within the recipient thymus Mls-1^a reactive T cells are eliminated from the developing thymocyte population that is derived from the donor bone marrow. In the present study we have investigated the tolerogenicity of Mls-1^a antigen derived from host T cells which represent a major population of radio-resistant cells in the [Mls-1^b -> Mls-1^a] chimeras. We found that recipient T cells which can survive lethal irradiation and produce intrinsic superantigens alter eventually the T cell repertoire in the thymus which have been developing from precursors of donor bone marrow.2. An NK1.1^+TCRalphabeta^<low> thymocyte subpopulation was identified. This subpopulation exhibited cytotoxicity against CD4^+8^+ immature thymocytes from syngeneic and allogeneic mice. The cytotoxicity was shown to be mediated by Fas-Fas ligand interaction.3. A monoclonal antibody (2H2) which is specific for a src-family tyrosine kinase, Fgr, has been established. We could idntify Fgr positive macrophages in lymph nodes and spleen. The Fgr was shown to be associated with Ly6C and seveeral proteins.4. A gretopic motifs on pigeon cytochrome c related peptides were determined. On the basis of these agretopic motifs, we could produce synthetic peptide vaccines against influenza viras.

1。MTV-7的表达，MTV-7的表达是内源性乳腺肿瘤病毒的遗传基因座，该病毒决定了较小的淋巴细胞刺激性（MLS-1^<a \ 0>表型）导致缺失表达VBETA 6、7、8.1或9中的细胞中的细胞，或者使用同异构骨骼中的胸骨骨骼。嵌合体，我们已经证明，在受体胸骨中，从供体骨髓中衍生出的胸腺细胞种群中消除了反应性T细胞发现可以生存致命的T细胞，并产生固有的超级抗原，最终改变了胸腺中的T细胞曲目，这是从供体骨髓的前体中发展的。2来自合成和同种异体小鼠。细胞毒性被证明是由FAS-FAS配体相互作用介导的3。已经建立了针对SRC家族酪氨酸激酶FGR的单克隆抗体（2H2）。我们可以在淋巴结和脾脏中iDnnnnnnnnnnnnnnnnnnnnnnnnt。 FGR被证明与Ly6c和Seveeral蛋白有关。4。确定了鸽子细胞色素c相关肽上的粒度基序。根据这些敏锐的基序，我们可以针对流感的Viras产生合成的肽疫苗。