Molecular Assembly and Stability of Human Hepatitis B Virus Surface Antigen Vaccine

人乙型肝炎病毒表面抗原疫苗的分子组装及稳定性

基本信息

批准号：
02453151
负责人：
TAKAGI Toshio
金额：
$ 1.92万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-02453151/
关键词：
vaccine Gene Engineering electrophoresis Light Scattering ヒトB型肝炎 X線小角散乱

项目摘要

Vaccines superior to the conventional ones can now be produced in principle using gene engineering technique. Human hepatitis B virus surface antigen vaccine derived from yeast(y-HBsAg vaccine)is, however, the first and only successful outcome of the introduction of the engineering technique into vaccine production. Its efficacy is believed to be derived from the particular nature and high stability of the assembly composed mainly of protein polypeptides and lipids. The present research project was motivated from our belief that the mode of assembly of the constituents and the source of the stability must be understood to provide basic knowledge hopefully leading to production other efficient vaccines through the approach.The y-HBsAg vaccine particles were found to be spherical, and homogeneous with respect to their size having radius of about 19 nm as judged from dynamic light scattering, small-angle X-ray scattering and electron microscopy. No regular structure was detected in the vaccine particle by small-angle X-ray scattering. The vaccine particle was eluted from a TSK-Gel G600OPW column as a symmetrical peak. Monitoring of the elution gave molar mass estimate of 5, 040 kg/mol for the vaccine particle of the target in the present study of which constituent polypeptide has 9 additional amino acid residues in the N-terminal side compared to the polypeptide of the virus surface antigen. The molar mass of the vaccine particle consisted of the latter polypeptide was estimated to be 4, 640 kg/ mol. The vaccine particle was stable against heating up to 8OﾟC. The constituent polypeptide also showed stability against heating in the presence of SDS. Isoelectric point of the particle was estimated to be 4.0 to 4.2 by electrophoretic light scattering in free solution. It is hoped that these results and approaches can play a role of guidepost for future characterization of vaccine particles expected to be developed in near future using gene engineering technique.

然而，超过传统的疫苗可以从年份（y-hbsag疫苗）衍生出来，但是，安迪（Andy）的第一个成功结果是将工程技术引入疫苗的产生从我们的信念中激发了成分组装方式和稳定性的来源，必须理解以提供基本知识，希望通过方法能够导致生产其他有效的疫苗。关于它们的大小约为19 nm，是从动态光散射中判断的，而不是电子结构在本研究中，洗脱的摩尔质量估计值为5个靶标的摩尔元素，即与病毒表面抗原的多肽相比，N末端的征兵多肽9个额外的氨基酸性。为4，640 kg/ mol。最多8o。分为技术的疫苗。