Developmental Endocrinology and Treatment of Ovarian Cysts in Cattle

牛卵巢囊肿的发育内分泌学和治疗

基本信息

批准号：
01480106
负责人：
MORI Junichi
金额：
$ 2.43万
依托单位：
University of Osaka Prefecture
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1990
项目状态：
已结题

项目摘要

Developmental endocrinology and treatment of ovarian cysts in cattle were examined, and the following results were obtained.1. Cows with ovarian cysts did not have remarkable abnormality in numbers and affinities of gonadotropin-releasing hormone (GnRH) receptors of the anterior pituitary.2. Numbers of gonadotropin receptors and concentrations of estradiol-17 beta in fluid of ovarian cysts were significantly lower than those of large follicles. The number of luteinizing hormone (LH) receptors in non-luteinized cysts (plasma concentration of progesterone <1 ng/ml) was significantly fewer than that of luteinized cysts (plasma concentration of progesterone *1 ng/ml). More estradiol-17 beta in fluid of non-luteinized cysts was observed when they possessed relatively many LH receptors.3. The addition of cortisol to granulosa cells in vitro caused a significant decrease in the unmber of LH receptors and the estradiol-17 beta production.4. The addition of progesterone to the median eminence o … More f the hypothalamus in in vitro perfusion experiments caused a significant decrease in the GnRH release.5. Ovarian cysts were experimentally induced by the treatment with adrenocorticotropic hormone (ACTH). During development of ACTH-induced cysts, LH and follicle stimulating hormone (FSH) surges from the pituitary which normally found prior to ovulation were disappeared. After ACTH treatment, the increase in plasma progesterone and cortisol levels accompanied by low estradiol- 17 beta level were observed, suggesting that these changes could be the reason for the disappearance of LH and FSH surges.6. Studies on the treatment of ovarian cysts with human chorionic gonadotrpin (hCG) revealed that the efficacy of the drug could be estimated to some extent by determining the concentrations of progesterone in peripheral plasma and estradiolー17 beta in fluid of ovarian cysts.These results suggest that, as a possible mechanism of development of ovarian cysts, cortisol and progesterone production from adrenal are increased by ACTH stimulation under continuous stress, and the former interferes with follicular maturation by decreasing the number of LH receptors and the estradiol-17 beta production in granulosa cell, while the latter inhibits directly GnRH release from the median eminence, thereby resulting in the disappearance of gonadotropin surge and ovulation failure. They also indicate that there is a constant relation between the efficacy of treatment and endocrinological status in cows with ovarian cysts. Less

对牛的发育内分泌及卵巢囊肿的治疗进行了检查，得到以下结果： 1．卵巢囊肿的奶牛垂体前叶促性腺激素释放激素（GnRH）受体数量和亲和力未见明显异常。 2．卵巢囊肿液体中促性腺激素受体的数量和雌二醇17β的浓度显着低于大卵泡。非黄体化囊肿（血浆黄体酮浓度<1 ng/ml）中黄体生成素（LH）受体的数量明显少于黄体化囊肿（血浆黄体酮浓度*1 ng/ml），雌二醇-17β较多。当非黄素化囊肿具有较多的LH受体时，在体外观察到在颗粒细胞中添加皮质醇会导致其显着减少。 LH 受体的数量和雌二醇-17 β 的产生。4. 在体外灌注实验中，向下丘脑中位隆起添加黄体酮可导致卵巢囊肿释放显着减少。5。促肾上腺皮质激素 (ACTH) 治疗引起的在 ACTH 诱发的囊肿形成过程中，LH 和卵泡刺激素 (FSH) 会激增。在ACTH治疗后，观察到血浆黄体酮和皮质醇水平升高，同时雌二醇-17β水平降低，这表明这些变化可能是LH和FSH激增消失的原因。 6. 人绒毛膜促性腺激素（hCG）治疗卵巢囊肿的研究表明，通过测定体内孕酮的浓度，可以在一定程度上评估药物的疗效。卵巢囊肿液体中的外周血浆和雌二醇−17β。这些结果表明，作为卵巢囊肿发生的一种可能机制，持续应激下 ACTH 刺激会增加肾上腺皮质醇和黄体酮的产生，而前者会干扰卵泡成熟通过减少颗粒细胞中 LH 受体的数量和雌二醇-17 β 的产生，而后者直接抑制中位隆起的 GnRH 释放，从而导致促性腺激素激增和排卵失败的消失还表明，卵巢囊肿奶牛的治疗效果与内分泌状态之间存在恒定的关系。