Effect of phytate degradation using phytase and soaking and resistant starch in barley on phosphorus utilization, gut health, and growth in pigs

使用植酸酶和浸泡抗性淀粉降解大麦中的植酸盐对猪的磷利用、肠道健康和生长的影响

基本信息

批准号：
398102117
负责人：
Dr. Charlotte Maria Elisabeth Heyer
金额：
--
依托单位：
Fachgebiet Tierernährung (460a)
依托单位国家：
德国
项目类别：
Research Fellowships
财政年份：
2018
资助国家：
德国
起止时间：
2017-12-31 至 2019-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/398102117?language=en
关键词：
Effect phytate degradation using phytase

项目摘要

The primary storage form of P in plant seeds is phytate (any salt of myo-inositol 1,2,3,4,5,6-hexakis (dihydrogen phosphate); InsP6) that is poorly available for non-ruminant animals, such as the pig. Depending on activity of phytases and other phosphatases, InsP6 is hydrolyzed down to less phosphorylated inositol phosphates, such as myo-inositol, and available P. Feed processing techniques such as soaking and fermentation that promote activation of phytases to reduce InsP6 concentration in feed have gained in importance. Furthermore, InsP6 is an anti-nutritional factor in pig feed by chelating positively charged cations and affecting utilization of other dietary nutrients. Results on the interaction between P and the immune system are inconsistent, several studies in different species have shown a positive impact of dietary P and phytase supplementation on the adaptive immune response. Recent studies indicate that individual inositol phosphates or phosphate may also affect properties of the intestinal microbiota, including pathogenic microorganisms. The proposed study assesses effects of inositol phosphate degradation (using soaking, fermentation and microbial phytase supplementation) and resistant starch (low vs. high fermentable barley cultivars containing high and low amylose, respectively) in situ and in vivo using pigs on P utilisation, gut health and growth. The milestones of the proposed research project are: (1) Evaluation of the enzymatic degradation of phytate in situ using soaking and fermentation; (2) Effects of diets containing phytase supplementation and low vs. high fermentable barley cultivars soaked with lactic acid bacteria on nutrient digestibility, and (3) Impact of diets containing phytase supplementation and low vs. high fermentable barley cultivars soaked with lactic acid bacteria on growth performance in nursery pigs with special focus on the gastro-intestinal microbiota and immune system. The advantages of the planned research work are: (1) Results of the metabolism of InsP6 and lower inositol phosphates in vivo can be used target further increase InsP6 digestibility, to reduce supplementation with phosphate and to minimize P pollution from manure; (2) Using processing strategies (soaking and fermentation) and the supplementation of microbial phytase to the diet improves the understanding of phytase activity of different origins, and evaluates potential synergistic effects; (3) Description of the complex relationship between InsP6 in combination with starch using a common cereal, the gastro-intestinal microbiota and the immune system can be used to beneficially affect swine health and performance.

植物种子中磷的主要储存形式是植酸盐（肌醇 1,2,3,4,5,6-六（磷酸二氢盐）的任何盐；InsP6），非反刍动物（如猪。根据植酸酶和其他磷酸酶的活性，InsP6 被水解为磷酸化程度较低的肌醇磷酸盐，例如肌醇和可用磷。饲料加工技术（例如浸泡和发酵）可促进植酸酶的活化，从而降低饲料中 InsP6 的浓度。的重要性。此外，InsP6 通过螯合带正电的阳离子并影响其他日粮营养素的利用，成为猪饲料中的抗营养因子。关于磷与免疫系统之间相互作用的结果并不一致，针对不同物种的多项研究表明，膳食磷和植酸酶补充对适应性免疫反应具有积极影响。最近的研究表明，单独的肌醇磷酸盐或磷酸盐也可能影响肠道微生物群的特性，包括病原微生物。拟议的研究使用猪在原位和体内评估了磷酸肌醇降解（使用浸泡、发酵和微生物植酸酶补充）和抗性淀粉（分别含有高和低直链淀粉的低发酵大麦品种与高发酵大麦品种）对磷利用、肠道的影响。健康与成长。该研究项目的里程碑是：（1）利用浸泡和发酵对植酸盐原位酶促降解进行评估；（2）添加植酸酶的日粮以及用乳酸菌浸泡的低发酵大麦品种与高发酵大麦品种对养分消化率的影响，以及（3）添加植酸酶的日粮以及用乳酸菌浸泡的低发酵大麦品种与高发酵大麦品种对养分消化率的影响保育猪的生长性能，特别关注胃肠道微生物群和免疫系统。计划研究工作的优点是：（1）InsP6和低磷酸肌醇在体内的代谢结果可用于进一步提高InsP6的消化率，减少磷酸盐的补充，最大限度地减少粪便中的磷污染；（2）采用加工策略（浸泡和发酵）以及在日粮中添加微生物植酸酶，可以提高对不同来源植酸酶活性的了解，并评估潜在的协同效应； (3) 描述 InsP6 与淀粉结合使用普通谷物、胃肠道微生物群和免疫系统之间的复杂关系，可用于有益地影响猪的健康和生产性能。