Time-resolved small-angle X-ray scattering of GUV deformed by F-BAR domain

F-BAR 域变形的 GUV 的时间分辨小角 X 射线散射

• 批准号: 24657078
• 负责人: NOBUTAKA Shimizu
• 金额: $ 2.58万
• 依托单位: High Energy Accelerator Research Organization
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24657078/
• 关键词: 生体膜変形; 時分割X線小角散乱; ストップトフロー; X線溶液散乱; 時分割測定; GUV; 脂質膜変形; X線小角散乱; 生体膜

Some proteins transform an intracellular organelle and maintain structure. It is important to study those interactions in order to understand the structure and the function of the organelle. Then, a stopped-flow was installed in the small-angle X-ray scattering beamline at the Japanese synchrotron facility, Photon Factory, and the structural change of Giant unilamellar vesicle (GUV) interacted by the BAR domain protein was measured by time-resolve method. As a result, although it was very preliminary analysis, it was suggested the structure of GUV was changed with BAR domain in the space scale of around 125 angstrom within 210 seconds.

一些蛋白质会转化细胞内细胞器并保持结构。重要的是要研究这些相互作用，以了解细胞器的结构和功能。然后，在日本同步加速器设施，光子工厂的小角度X射线散射光束线中安装了一个停止流，并通过时间分解方法测量了由条形域蛋白相互作用的巨型Unilamelar囊泡（GUV）的结构变化。结果，尽管这是非常初步的分析，但建议在210秒内，在大约125 Angstrom的空间尺度上，GUV的结构被更改。

项目成果

Current Status of Small Angle X-ray Scattering Beamlines at Photon Factory

光子工厂小角X射线散射束线现状

• 发表时间: 2012
• 作者: Shimizu;N.;Mori;T.;Igarashi;N.;Ohta;H.;Nagatani;Y.;Kosuge;T. and Ito;K.
• 通讯作者: K.

PF BL-15A：高輝度 XAFS/XRF/SAXS ビームライン

PF BL-15A：高亮度 XAFS/XRF/SAXS 光束线

• 发表时间: 2014
• 作者: 五十嵐教之;小山篤;森丈晴;上條亜衣;西條慎也;大田浩正;仁谷浩明;丹羽尉博;阿部 仁;清水伸隆
• 通讯作者: 清水伸隆

Refurbishment of SAXS beamlines at Photon Factory

Photon 工厂 SAXS 光束线翻新

• 发表时间: 2013
• 作者: 五十嵐教之;小山篤;森丈晴;上條亜衣;西條慎也;大田浩正;仁谷浩明;丹羽尉博;阿部 仁;清水伸隆;Nobutaka Shimizu
• 通讯作者: Nobutaka Shimizu

BL-15A : Semi-microbeam XAFS/XRF and High- brilliance SAXS beamline at Photon Factory

BL-15A：Photon Factory 的半微束 XAFS/XRF 和高亮度 SAXS 光束线

• 发表时间: 2013
• 作者: Igarashi;N.;Koyama;A.;Mori;T.;Ohta;H.;Kamijo;A.;Saijyo;S.;Niwa;Y.;Nitani;H.;Abe;H. and Shimizu;N.
• 通讯作者: N.

Structure of liposome encapsulating proteins characterized by X-ray scattering and shell-modeling.

• DOI: 10.1107/s0909049513020827
• 发表时间: 2013-11
• 期刊: Journal of synchrotron radiation
• 影响因子: 2.5
• 作者: Hirai M;Kimura R;Takeuchi K;Hagiwara Y;Kawai-Hirai R;Ohta N;Igarashi N;Shimuzu N
• 通讯作者: Shimuzu N