Functional signals and hologenomic selection for P utilisation and related efficiency traits in two contrasting high-yielding laying hen strains

两种对比高产蛋鸡品系磷利用和相关效率性状的功能信号和全基因组选择

• 批准号: 386031347
• 负责人: Professor Dr. Jörn Bennewitz
• 金额: --
• 依托单位: Fachgebiet Tierernährung (460a)
• 依托单位国家: 德国
• 项目类别: Research Units
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/386031347?language=en
• 关键词: Functional; signals; hologenomic; selection; utilisation

In a recent study, we conducted quantitative-genetic analyses of Phosphor (P) utilization in an F2 cross experimental design consisting of about 900 Japanese quails. We estimated a narrow sense heritability of 14 percent. The individuals were genotyped with 4K SNP markers and lumen samples from the ileum were collected for microbiota analyses. The overall aim of the present project is to obtain a better understanding of the P utilization observed in the study design considering the quail genetics, the quail ileum microbiota and the interplay between these two components. In particular, we aim to test the following hypotheses. (i) Variation of P utilization in quails is a heritable trait, which is controlled by few quail QTL with large effects. (ii) Variation of P utilization is partly driven by the microbial community in the ileum of the quail. (iii) The microbial community in the ileum is a heritable host trait that can be used for breeding individuals with improved P utilization. These hypotheses will be analyzed by using genomic and microbial mixed linear models, which include QTL linkage and association mapping, genomic and microbial variance component estimation, and microbial prediction of P-Utilization. The microbiota will be analyzed using 16S rRNA amplicon sequencing technology. The results will be interpreted with respect to improve P utilization in poultry by targeted breeding, which acts either on the level of the observed P utilization of the host, or on the host microbiome in order to select for an optimized microbiota with respect to P utilization, or on both. A wider interpretation, which uses bioinformatics tools and considers the results from other projects of this Research Unit into account, will be done in another Research Unit project.

在最近的一项研究中，我们在由约 900 只日本鹌鹑组成的 F2 杂交实验设计中对磷 (P) 的利用进行了定量遗传分析。我们估计狭义遗传率为 14%。使用 4K SNP 标记对个体进行基因分型，并收集回肠管腔样本进行微生物群分析。本项目的总体目标是更好地了解研究设计中观察到的磷利用，考虑鹌鹑遗传学、鹌鹑回肠微生物群以及这两个组成部分之间的相互作用。特别是，我们的目标是检验以下假设。 (i)鹌鹑磷利用变异是一种遗传性状，受少数鹌鹑QTL控制，影响较大。 (ii) 磷利用率的变化部分是由鹌鹑回肠中的微生物群落驱动的。 (iii) 回肠中的微生物群落是一种可遗传的宿主性状，可用于培育具有改善的磷利用率的个体。这些假设将通过使用基因组和微生物混合线性模型进行分析，其中包括 QTL 连锁和关联作图、基因组和微生物方差分量估计以及 P-利用率的微生物预测。将使用 16S rRNA 扩增子测序技术对微生物群进行分析。结果将被解释为通过有针对性的育种提高家禽的磷利用率，该育种要么作用于观察到的宿主磷利用率水平，要么作用于宿主微生物组，以便选择磷利用率方面优化的微生物群，或两者兼而有之。更广泛的解释将在另一个研究单位项目中进行，该解释使用生物信息学工具并考虑该研究单位其他项目的结果。